Aims and objectives- Though, there is clear-cut right dominance in upper extremity; reports on lower extremity are ambiguous. Some authors observed preponderance of stronger thigh bones on right side others reported longer and heavier femurs on left side. Therefore, it was considered worthwhile to report our observations on bilateral variability of thigh bones from Marathwada region and to derive the percent directional asymmetry allowing for direct comparison of asymmetry in various dimensions. Materials and methods- Present study was carried out on 168 known sex femurs from bone bank of the Department of Anatomy, Government medical college, Aurangabad. All the femurs were dry, free of damage and deformity and are fully ossified. 11 bilateral dimensions were taken. Dimensions were converted into percent directional asymmetry. Results and Conclusion- All the dimensions were higher on left side; but for the diaphyseal dimensions difference was significant. Percent directional asymmetry reveals that males have more bilateral asymmetry than females. Antero-posterior and transverse diameters of mid-shaft showed higher percent directional asymmetry. The probable reason for bilateral variation is asymmetrical mechanical loading and different behaviour patterns.