Aim: The aim of this study is to determine the gender of an individual by handwriting Methodology: In this study total 130 handwriting samples were collected and analyzed. In whichF__ampersandsignnbsp;65 males and 65 female volunteers of age group 18 to 30 years were selected. Handwriting samples are taken on A - 4 Size paper of London Letter. Exclusive factors in this study are only literate, young and healthy persons are included. Result: Macro features and micro features were examined in all the 130 handwriting samples in both male and females by using feature extraction method and z- tests. In the second method which is Hypothesis testing done by z-test, Null hypothesis was taken that there is no significant difference in male and female handwriting and on the basis of value of z in table - 2 at 5% level of significance with critical value of 1.96, out of 27 features for which z-test was performed, Seven features could said to be gender identifying features and may help in identifying the gender of the writer. Conclusion: On the basis of above result, feature extraction and z- test may said to be the two methods which may explain about the utility of these tests for examination of hand writing for gender identifying feature. It can be said that there is a significant differences between hand writing of male and female and hence a handwriting sample can also be examined for gender identification purpose.