Introduction: The advancements and applications of redundant tissue sources such as human subcutaneous adipose tissue (SF) and human umbilical cord matrix (HUCM) tissue are gaining importance in recent years. Despite these advancements, certain stumbling blocks accounts for lack of functional improvement of the diseased. One major bottleneck for the prevailing failures relies on understanding the migratory and homing potential of stem cells. Cell adhesion molecules have been identified to play a vital role in vascular adhesion, migration, extravasations and ultimately homing. However, significance of cell adhesion molecules in therapeutic implications has not much concentrated upon in recent years unlike mesenchymal stem cells. Objective: The present study showcases the significance of cell adhesion molecules and addresses the major issue on identity of an ideal stem cell source with maximum benefits. Research methodology: To this end, the cells obtained from the human SF and HUCM were cultured until P3 and cultured cells were characterized for comparative expression profile analysis of certain cell adhesion molecules. Outcome of the study: Cultured MSCs derived from both these aforesaid sources exhibited a significant percentage of cell adhesion molecules, thereby substantiating its efficacy on tissue homing and migration. Thus, both the sources were found superior with regards to the expression of CAM and can be clinically exploited. However, umbilical cord matrix serves a better therapeutic option for allogenic transplantation, which is evident from the sparse expression of CD 34 at primary culture itself, thereby opening a gateway to circumvent the surgical complications in clinical transplantations.