Objective: The study was conducted to evaluate the accuracy, cost and turnaround time of wet mount, gram stain and acridine orange stain microscopy of quantitative unspun urine for screening of urine samples. The combination of wet mount and gram stain and wet mount and acridine orange stain was also evaluated. Materials and Methods: A total of 618 urine samples, which comprised of first ten consecutive samples that were received in the microbiology laboratory daily from patients with suspected urinary tract infection, were included in the study. All uncentrifuged urine specimens were subjected to wet mount, gram__ampersandsignrsquo;s stain, and acridine orange stain microscopy and semi quantitative urine culture tests. Time taken and expenditure for each test per sample was calculated and compared. Result: Sensitivity, specificity, PPV and NPV of all the screening tests were compared singly and in combination. Acridine orange and gram stain had the highest sensitivity while wet mount, wet mount+gram stain and wet mount+acridine orange stain showed similar sensitivities. Specificity was highest for wet mount+acridine orange followed by acridine orange, wet mount+gram stain, gram stain and wet mount. PPV was highest for wet mount+acridine orange followed by acridine orange, gram stain, wet mount+gram stain and wet mount. NPV was highest for acridine orange followed by wet mount+acridine orange, gram stain, wet mount+gram stain and wet mount. All the microscopic tests were significantly rapid and cost effective as compared to culture test. Conclusion: Acridine orange stain is recommended as a single screening test as it is an accurate, rapid and inexpensive method to rule out UTI.