Objectives: The objectives of this study are to assess the knowledge, attitude and practice regarding blood donation among various categories of health professionals and comparing them with that of the general population. Material and method: A cross sectional descriptive study was carried out at Pondicherry. Total 150 individuals were interviewed including doctors (n=25), nurses (n=25), technicians (n=25) and general population of Pondicherry (n=75). All were interviewed with the help of a pretested and semi-structured questionnaire. Results: There was a significant difference in knowledge pertaining to blood donation between health professionals and the general population. On the other hand there was no difference of attitude of the two groups. There was no difference in knowledge and attitudes according to gender but most of the donors were males. Conclusion: There is a need to increase the awareness and attitude among the paramedical staff and in general population. There is also need to motivate females for blood donation. There is also need to create awareness about blood safety in paramedical staff and general population.