Objective: Resistance to antipseudomonal antibiotics is increasing worldwide. Area-wise studies on antimicrobial susceptibility patterns are essential to guide policy on the appropriate use of antibiotics. The present study was conducted to find out the current antimicrobial susceptibility pattern of P.aeruginosa isolates obtained from various clinical samples at our hospital. Methods and Material: The present study was conducted in a tertiary care hospital in Rajahmundry, Andhra Pradesh , India , during Jan 2011 __ampersandsignndash; Jun 2012. 107 clinical isolates of P.aeruginosa obtained from various clinical samples were studied. They were identified by Routine standard operative procedures. Antimicrobial susceptibility testing was done by Kirby-Bauer disk diffusion method and the results were interpreted according to the CLSI guidelines.Data obtained was analysed and presented in counts and percentages. 95 % confidence interval and p values were also calculated as applicable. Results: Imipenem was the most active antibiotic with 95.3 % susceptibility rate followed by Piperacillin-Tazobactem [89.7% ] and Levofloxacin [ 84.1% ] . Amikacin showed better susceptibility rate [74.7%] when compared to that of Gentamicin [65.4%]. The susceptibility rates to Cephalosporins and Aztreonam were relatively low. Most of the P.aeruginosa strains were isolated from clinical samples like pus [32.7% ],urine [ 26.1% ], and respiratory secretions [ 22.4% ]. 29 [27.1%] out of 107 clinical isolates of P.aeruginosa were found to be multidrug resistant. Most of the MDR P.aeruginosa strains were isolated from pus, urine, and respiratory secretions. Conclusions: The overall resistance demonstrated by clinical isolates of P.aeruginosa is high. This can be attributed to the inappropriate use of antibiotics. Guidelines on appropriate use of antibiotics not only reduces the morbidity and mortality in the patients infected with P.aeruginosa but also controls the emergence and spread of resistance among these pathogens.