The present investigation was undertaken to study the effect of different concentrations of BAP, Kin, Ads on shoot induction in Asparagus Racemosus. There was shoots induction after 3 weeks when Nodal explants segments were cultured on basal medium supplemented with BAP, Kin and Ads. Shoots induction on MS basal medium supplemented with BAP (1 mg/l) + kinetin (1 mg/1) were transferred to MS basal medium containing BAP (1.5mg/1) + Ads (100mg/1) and BAP (1.5mg/1), Kin (l mg/1) respectively to the Proliferation shoots. Stem segments cultured on MS basal medium supplemented with various concentrations of BAP (0.75, 1.5,1.0 mg/1), AdS (50,100 mg/l) and Kin (0.75,1.0,1.5 mg/l) induce shoot, But a combination of l mg/1 BAP + 1 mg/l Kin and 1.5 mg/1 BAP + 100.mg/1 Ads showed highest percentage of shoot proliferation after 7-14 days.