Lawsonia inermis (Lythraceae) is commonly called as Heena. Lawsonia inermis L. is a medicinal as well as commercially used plant. It has been found that conventional methods of propagation of L. inermis, sexual as well as vegetative, are beset with many problems that restrict their multiplication on a large scale. Propagation through seed is unreliable because of disease and pest problems, short viability and heavy rains during the seeding season in the natural habitat. Therefore, it is necessary to use in vitro propagation for rapid plantproduction. For shoot formation both apical shoot buds and meristems were used. This was achieved on MS medium containing different concentration of BAP alone or in combination with Kinetin was tested. Best shoot initiation response was achieved on MS Medium supplemented with 0.2 mg/l BAP + 0.2 mg/l kn on both apical shoot and axillary meristem explants.