Background of study: Oral candidiasis is the most common opportunistic infection in HIV seropositive patients, also predictive of immunosuppression. Though Candida albicans is the predominant isolate in oral candidiasis, there is rise in non albicans Candida infection coupled with high levels of antifungal resistance. There is urgent need for rapid, simple and reliable method to identify yeast isolates. Hicrome agar- Candida is a selective and differential medium for identification of yeasts directly from clinical samples. This medium allows selective isolation of yeasts and simultaneously identifies certain species of Candida. Aim / Objective: To evaluate the utility of Hicrome agar-Candida in identification of C. albicans and non albicans Candida. Research Methodology: Two oral swabs obtained from 100 HIV seropositive patients having oral candidiasis were subjected to identification and characterization by standard conventional methods. Simultaneously direct inoculation was done on Hicrome agar- Candida plate. Conclusion: Of the total 100 samples 103 species were obtained. C. tropicalis was the most common species isolated followed by C. guilliermondi, C. parapsilosis, C. kefyr, C. albicans, C. krusei, C. glabrata, C. fomata and C. pelliculosa. Hicrome agar showed selective growth of all Candida species with distinguishing color for each species. C. tropicalis showed blue color with sensitivity (68%) and specificity (98.72%). C. albicans showed green colored colonies with 100% sensitivity and specificity respectively. C. kefyr showed pink color with sensitivity (61%) and specificity (92%). C. guilliermondi showed 95% sensitivity and 59% specificity. Hicrome agar differentiated mixed culture with all samples.