Aim: Tannery industry is one of the important industry that pose environmental pollution through the disposal of its effluent. Hence this study was carried out to analyze physico chemical parameters of the effluent already treated by the industry (industry treated effluent) to degrade the pollutants of the effluent using fungi and finally to reuse the biotreated sample for the agriculture purpose. (ie) growth of vegetable plant Lycopersicum esculentum and growth parameters were studied. Biochemical characterization (ie) proteins and DNA present in the roots of Lycopersicum esculentum were also studied using electrophoresis. Methodology: 5 litres of industry treated tannery effluent was collected in polythene containers from the tannery treatment plant situated in Chennai, Tamil Nadu India. The samples were collected for a period of 3 months from July 2015 to September 2015. The physico-chemical parameters were determined. Biotreatment of industry treated tannery effluent was carried out using native fungi Aspergillus niger and Rhizopus sp. Biotreated tannery effluent was used for growth of vegetable-tomato plant Lycopersicum esculentum for a period of 60 days and biochemical characterization such as protein and DNA of plant were studied by using electrophoresis. Result: The results of physico-chemical characteristics of the industry treated tannery effluent has revealed that the effluent was brownish in colour with offensive odour, pH was alkaline in nature and has higher concentration of EC, BOD, COD, TSS and TDS though the effluent was treated by the industry which surpassed the permissible limits prescribed by CPCB (1995). The results of microbial analysis of the effluent revealed the occurence of 4 species of fungi in the industry treated tannery effluent namely, Aspergillus niger, Mucor sp, Pencilluim sp and Rhizopus sp. The results of analysis of degradation of the effluent showed that colour and odour of 100% treated sample has changed to colourless nature and odorless condition and also other pollutants were reduced using native fungi, Aspergillus niger and Rhizopus sp. The results of the plant growth showed that Lycopersicum esculentum was increased in bio-treated and control compared to industry treated sample. Conclusion: The study revealed that native fungi, Aspergillus niger and Rhizopus sp plays a key role in the degradation of industry treated tannery effluent and the treated water can be utilized for agricultural purposes.