Objective: Primary open angle glaucoma is the most commonest form of glaucoma which leads to vision loss if untreated. It has been hypothesized that oxidative damage may be involved in the pathogenesis of glaucoma and homocysteine may also have a role in development of glaucoma. The aim of our present study is to evaluate the role of oxidative stress and Homocysteine in Primary open angle glaucoma patients. Methods: We tried to assess Serum Homocysteine levels and the role of oxidative stress in patients by estimating the levels of lipid peroxidation assessing plasma Malondialdehyde (MDA) levels and antioxidant status by reduced glutathione (GSH), Vitamin-E ,Vitamin-C in blood. For this, we have taken 50 cases of primary open angle glaucoma patients compared with 75 age matched controls . Results: There were significant increase in the levels of MDA and significant decreases in the levels of antioxidants like GSH whereas there are significant increases in the levels of vitamin-E and Vitamin-C in breast cancer patients when compared with controls. No statistical significant difference was observed with Serum Homocysteine levels in cases when compared with controls. Conclusion: Our results indicate that oxidative stress is associated with the development of Primary open angle glaucoma which needs further studies.