Chemical control for pests has been a serious concern for human and animal health; therefore this study was undertaken to screening of entemopathogenic fungi that are effective against against Greater wax moth Galleria mellonella. entemopathogenic fungi were isolated from different agricultural soil samples by plate dilution and baiting techniques. The crude extract of isolated fungi were screened against G. mellonella. Applied different fungal crude extracts of iolates achieved variable results on both larval and adult mortality percentages. The effect of crude extracts of Beauveria bassiana (Siwa isolate), Aspergillus tamarii, Fusarium solani, F. oxysporum, F. chlamydosporum and B. bassiana (El-Farafra isolate) giving total death percentages (82.48, 45.36, 37.12, 49.48, 53.6 and 74.22, respectively) to G. mellonella larvae comparing with the control treatment 0%. The results exhibited significant effects of B. bassiana (El-Farafra and Siwa Oasis isolates) against G. mellonella (larval and pupal weights). The % of larvae death increased with increasing the concentration of crude extract of B. bassiana (El-Farafra and Siwa isolates). Also pupal death percentage of G. mellonella increased while adult emergence decreased with increasing concentration of crude extract of El-Farafra and Siwa isolates of B. bassiana. On the other hand, crude extract of B. bassiana (El-Farafra isolate) was more active than B. bassiana (Siwa isolate) at high concentration (50 mg/ 0.5ml) while at other concentration crude extract of B. bassiana (Siwa isolate) was more active than B. bassiana (El-Farafra isolate). At the same time, different fractions of B. bassiana (Siwa isolate) extract were tested to determine the active metabolite. The bioactive compound was separated and identified as Beauvericin with using Thin layer chromatography, Fourier Transform Infrared (FT-IR) and analytical X ray.