The present investigation was designed to investigate associated fungi and deterioration of chemical constituents in drug Solanum indicum Linn. roots under influence of different relative humidities. Associated fungi isolated and identified according standard references. In the present study, total 20 fungi were associated with roots. F. semitectum, F.__ampersandsignnbsp; xysporum and F. solani showed high percentage incidence while minimum percentage incidence recorded in case of Sordaria fimicola, Neurospora sitophila, Thraustotheca sp., A. flavus, Aphanomyces sp., Chaetomium globosum and Rhizopus oryzae. The drug stored under the influence of different relative humidities viz. 30, 50, 75, 96 and 100% showed variation in percentage incidences of fungi as well as biodeterioration of chemical constituents such as sugars, proteins, phenols and glycosides. The drug stored under high relative humidity 96 and 100% and with the length of incubation days 45 and 60 days showed maximum deterioration of chemical constituents.