Objectives: Anatomical brain asymmetries in cadaveric human brains are not well defined in the literature. Therefore, the present study was undertaken to observe the variations in right and left cerebral hemispheres using various parameters. Methods: Twenty eight adult human brains irrespective of sex were taken for the present study. Various measurements of cerebral hemisphere including fronto-occipital length, cerebral width, Sylvian fissure length and distance between anterior Sylvian point to inferior Rolandic point were taken. These measurements were taken by Vernier calipers. Different configuration of the anterior ascending and anterior horizontal ramus of the Sylvian fissure were also examined as U-shaped, V-shaped and Y-shaped. The presence or absence of triangular sulcus located within pars triangularis was also observed. Results: The fronto-occipital length was longer on right side while the cerebral width and Sylvian fissure length were more on left side. These values were significant statistically. The configuration of the anterior ascending and anterior horizontal ramus of the Sylvian fissure was V-shaped in maximum number of hemispheres followed by U-shaped. The triangular sulcus was noted in thirty hemispheres i.e. 16 right and 14 left. Conclusions: Hemispheres of the brain are not identical and functional asymmetries have an underlying anatomical basis.