Aim: Dentinal hypersensitivity is an unpleasant symptom which occurs because of dentin exposure as a result of attrition, erosion, abfraction, and gingival recession. There are various treatment modalities to treat dentinal hypersensitivity. Iontophoresis is one of the popular technique which allows a concentrated application of drugs into the desired localized region of exposed dentine there by reducing the dentinal hypersensitivity. Case Report: This article describes about treatment of patient with dentinal hypersensitivity using Iontophoresis technique. Discussion: Immediate relief from dentinal hypersensitivity was achieved by clogging the dentinal tubules with usage of APF (Acidulated phosphate fluoride) gel by this iontophoresis technique. Conclusion: Six months follow up study of this patient showed the long lasting effects of this technique, achieved by deeper penetration of fluoride ions. So it can be used as a first line of treatment in dentinal hypersensitivity.