Background: Oxidative stress has been implicated as a mediator of cartilage damage in patient with Osteoarthritis (OA). Aim: To assess the antioxidant markers like superoxide dismutase (SOD) , glutathione peroxidase (GPx) and uric acid (UA) in the blood and cartilage metabolic markers like hyaluronic acid (HA) and keratan sulphate (KS) in the synovial fluid of osteoarthritic patients and to find the correlation between these parameters in the blood and synovial fluid. Methods: This was an observational study conducted in the department of Biochemistry, Sikkim Manipal Institute of Medical Sciences (SMIMS), Gangtok. 75 osteoarthritic patients and healthy volunteers were taken for the study. Blood levels of antioxidants (SOD, GPx and UA) were assayed in both osteoarthritic and healthy volunteers. Cartilage metabolic markers like HA and KS were assayed in the synovial fluid. Result: Osteoarthritic patients showed statistically significant increase in KS and decrease in HA level indicating cartilage damage. Decrease in SOD and GPx activities indicates increase in oxidative stress. The difference in UA levels in the two groups was not statistically significant. This study did not establish any significant positive/negative correlation between the antioxidants in the blood and cartilage metabolic markers in the synovial fluid of osteoarthritic patients.