Background: The importance of blood safety in public health was recognized long ago and data are essential to plan strategies to improve its status. Ability to ensure an adequate blood supply depends on knowing and understanding donor demographics and characteristics and their impact on collections. Objectives: To explore and compare the demographic profile and seropositivity among donors donating whole blood at fixed and mobile donation sites. Methods: The information of all the donors registered and donated blood was collected from the donor registration forms. Serological results of the donors were obtained from the Transfusion Transmitted Disease Screening division of the department. Data were entered into Microsoft excel sheet and results analyzed on SPSS software version 10. Results: In fixed site female donors contributed for about 1% of donations while at the mobile site their participation attributed to 18% of donations. Seropositivity was slightly higher among donors at fixed site (HBV-2.5%, HIV-1.1%) when compared to donors at the mobile site (HBV-1.8%, HIV-0.6%). The vasovagal reactions among donors were around 4% in fixed site and around 3% at mobile sites.