Background: Anemia is a disorder characterized by reduction in hemoglobin concentration of blood below the normal level. It is a global problem, mainly affecting poor people in developing countries. Methods: to determine trace metals fifty intravenous blood samples anemic patients and normal subjects were collected and immediately centrifuged to obtain the supernatant liquid, serum of both the groups for analysis. Results: The mean serum levels of magnesium, zinc, copper, and iron and antioxidant activity in anemic patients were 16.05__ampersandsignplusmn;1.14ppm, 4.33__ampersandsignplusmn;0.28 ppm, 2.5__ampersandsignplusmn;0.2ppm, 1.50__ampersandsignplusmn;0.28ppm and 1.27__ampersandsignplusmn;0.05ppm respectively. Serum magnesium, zinc, iron and antioxidant levels were significantly lower whereas, the higher level of Copper was observed in anemic patients compared to the controls. __ampersandsignnbsp;Conclusion: Supplementation with deficient trace elements may reduce the severity of symptoms and complications associated with anemia.