Introduction: Ovarian cancer is the fifth most lethal malignancy in women in the United States and second most common of all genital cancers in developing countries including India .Ovarian cancer is curable in early stages, and seldom curable in advanced stages. Objective: Study was conducted to determine (1) whether pre operative serum level of CA-125 could differentiate between Benign and Malignant neoplasm (2) to evaluate the significance of serum salivary amylase in ovarian neoplasm. Material and Methods: This was a cross sectional descriptive observational study conducted in a tertiary care hospital in north Kolkata, West Bengal, India. Detailed information regarding associated diseases and investigation reports were recorded in a case record form. Results: Serum CA-125 was analysed with pre-operative serum sample in 112 cases of ovarian neoplasm (Benign ???? 73, Malignant ???? 39). Serum level of CA-125 was significantly (p=0.0001) raised in malignant cases compared to benign neoplasm. The sensitivity and specificity of serum CA-125 was 92.3% and 87.6% respectively. In our study 92.3% of malignant tumour cases had raised level of CA-125.Serum salivary amylase was significantly (p=0.0001) more among malignant tumours (refer table-5). Conclusion: To conclude, in the present study there was significant increase of both serum CA-125 and salivary amylase level in malignant tumour cases than that of benign tumour cases.