Background: Racial differences in serum lipid and lipoprotein levels exist and assumed to be important determinants of cardiovascular disease. The purpose of this study is to investigate if there are any differences in serum lipid and lipoprotein level in the population of two different races of Bangladesh. Materials and Methods: Ninty one Chakma subjects (43 male and 48 female) and equal number of Bangalee subjects (39 male and 52 female) were randomly selected. Serum Total Cholesterol (TC), Triglyceride (TG), High Density Lipoprotein Cholesterol (HDL-C) of the study subjects were measured using commercial kits and Low Density Lipoprotein Cholesterol (LDL-C) was calculated. Result: The Bangalee subjects had higher levels of TC, TG, LDL-C and lower level of HDL-C than the Chakma population. The Bangalee males had higher TC, TG and LDL-C than the Chakma male. No significant difference was observed for HDL-C. Bangalee female had higher TC and TG and lower HDL-C than the Chakma female. No significant difference was found for LDL-C. Conclusion: This study clearly showed that racial variations exist in serum lipid and lipoproteins levels between the Bangalee and Chakma population of Bangladesh. Keywords: Racial variation, Lipid, Lipoprotein, Bangalee, Chakma.