The study was undertaken to identify suitable antibiotic and their required concentrations for enhancement of callus production and for further transformation studies. The effect of antibiotics such as carbenicillin, cefotaxime and streptomycin were studied on the callus growth of Terminalia chebula. Different antibiotics were added at different concentrations to the callus culture media and maintained up to 45 days. The experiments exhibited varied growth stimulation over controls. Cefotaxime and carbenicillin exhibited higher growth index when compared to control at different concentrations whereas streptomycin showed insignificant effect on growth index of Terminalia chebula callus culture. The results could be attributed to the plant hormone like chemical nature of cefotaxime and carbenicillin. However streptomycin did not exhibited higher callus production. Hence, it is concluded that the addition of antibiotics at optimum concentrations to the culture media could increase the growth of callus and the higher yield of callus.