Introduction: Hepatitis B is a common infection worldwide. Hepatitis B Virus and HIV share common modes of transmission and infection risk groups. Many HIV-positive individuals have also been exposed to hepatitis virus (HBV). The present study was undertaken to estimate the prevalence of HBsAg in HIV positive individuals attending VIMS, a tertiary care centre in Bellary, Karnataka. Materials and methods: A total of 875 individuals comprising of 440 males and 435 females HIV positive individuals referred from Anti Retroviral Therapy (ART) centre were screened for HBsAg in the serology section of the Department of Microbiology over a period of six months from January to June 2009. Serum samples collected from the patients included under the study were tested using HBsAg Immunochromatography rapid kits (Beacon Diagnostics) as per the manufacturer’s guidelines. The kits show a control line and test line in positive cases and only a control line in negative cases. Results: 46 serum samples tested positive for HBsAg (5.26%), of whom 26 were males and 20 were females. 27 of the 46 positive cases (58.69%) were in the age group of 31-40, of whom 20 were males and 7 were females. Conclusion: The present study showed a high percentage of HIV positive individuals in Bellary having co-infection with HBV with a predilection to males of sexually active age group. We recommend that all HIV positive individuals must be screened for HBV co-infection.