Introduction: The brachial artery is the principal artery of the arm. Variations in course and branching patterns of brachial artery are important for physicians, plastic and vascular surgeons and radiologists. Case Report: In the present case, unilateral trifurcation of brachial artery into radial, ulnar and superior ulnar collateral artery was observed in the right upper limb of an adult female cadaver. Conclusion: Our case appears to be unique in that the brachial artery was trifurcating at a higher level into ulnar, radial and superior ulnar collateral arteries. Health specialists must be aware of the possible variations of the brachial artery as it has several clinical applications.