Introduction: India has an overwhelming diabetic population and the importance of HbA1c testing is felt. However the need of an additional EDTA sample and due to lack of standardised laboratories in most part of India, samples are stored and transported for testing. The study aims to check the variation in HbA1c measurement in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA anticoagulant vials and check for its stability when sample are stored at 2 __ampersandsignndash; 8__ampersandsigndeg;C for 7 days. Methods and materials: HbA1c was measured by cation exchange HPLC based BioRad D - 10 analyser after collection of sample in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA from 25 diabetic and 25 non - diabetic subjects on day 1 and on day 3, day 5 and day 7 of sample collection, after storage at 2 __ampersandsignndash; 8__ampersandsigndeg;C. Result: There were no variation in the measured value of HbA1c in these anticoagulant vials (CV = 0.0 __ampersandsignndash; 0.8%). There were no significant differences in the mean values of the HbA1c measured on day 1 and day 7 (8.2 __ampersandsignplusmn; 1.16 vs. 8.2 __ampersandsignplusmn; 1.18, P = 0.962 in diabetic and 5.3 __ampersandsignplusmn; 0.25 vs. 5.3 __ampersandsignplusmn; 0.24, P = 0.955 in non - diabetic). There were significant correlations between the HbA1c values measured on day 1 and after 7 days storage (r = 0.991 in diabetic and r = 0.957 in non __ampersandsignndash;diabetic). Conclusion: HbA1c values in fresh and stored whole blood sample does not change when analysed in K3EDTA, Na-citrate, lithium-heparin and Na-fluoride/Na2 EDTA anticoagulant vials.