Screening and isolation of siderophore producing bacterial strains were carried out from soil samples collected from plant rhizosphere region. The isolate showed positive for siderophore on Chrome azurol sulfonate agar medium was selected for further studies. The organism was subjected to various biochemical tests and 16S rRNA analysis, which lead to its identification as Azotobacter sp. The Azotobacter sp. holds optimal growth temperature and pH at 30__ampersandsignordm;C and 8.5 respectively. It was also optimized for carbon and nitrogen with optimal growth in Malic acid and peptone respectively. The presence of magnesium and zinc with iron concentration of 4m concentration also favored the siderophore production. Siderophore production was carried out using optimized media and the harvested siderophore was partially purified by ion exchange chromatography. The antagonist activity of the siderophore producing Azotobacter sp. and partially purified siderophore were tested against fungal pathogens such as Fusariurm sp., Alternaria sp., Phytophthora sp., Rhizoctonia sp., Colletotrichum sp. and Curvularia sp. This result showed that the Azotobacter sp. under study is a good producer of siderophore, which can be beneficial for its antagonistic activity towards fungal pathogens.