In the field of forensic medicine, normally the available materials after sufficiently long period of death will be utilized to determine various body characteristics such as age sex etc for identification of individual.Identification of corpses is a difficult forensic procedure and it is mandated by laws and social rules. Comparison of ante mortem and post mortem medical records, such as dental documents, plays an important role in the identification of corpses. Gender has long been determined from the skull, the pelvis and the long bones with an epiphysis and metaphysic in unknown skeletonsaccording to krogmann. The methods such as 3D CT scan have been utilized to determine the gender. Objective: The aim of the present study is to evaluate size and volume of maxillary sinus to determine gender by 3D CT Scan method. This work is of National importance in identifying the sex of a person in the forensic medicine and also for criminal investigations. Method: The skulls of known sex were obtained from recently buried bodies. Initially skulls were scanned by 3D Multiaxial CT scan and dimensions and volume of maxillary sinuses were observed by using dedicated software. Results: The preliminary analysis of data discriminative by CT method has been tabulated. The volume of the maxillary sinuses of both sides was significantly greater in males compared to female skulls. The p value of left width and right sided volume of maxillary sinuses 0.015 and 0.021 respectively were considered statistically significant. Computerized tomography measurements of maxillary sinuses may be useful to support gender determination in forensic medicine.