Industrialization, globalization __ampersandsignamp; green revolution in agriculture have increased the demand for the petroleum products. Petroleum fuels release more emissions like CO, NOX, CH4 __ampersandsignamp; CFC which are responsible for global warming __ampersandsignamp; ozone layer depletion. Biodiesel is a less pollutant, biodegradable and renewable alternative fuel that can be used with little modifications. The objective of this study was to investigate the effect of the biodiesel produced from linseed oil on single cylinder diesel engine. Both performance and emission characteristics were studied in the range of one fourth load to full load at fixed compression ratio 16 . In the current research work biodiesel is prepared from linseed oil. The Experimentation was conducted on four stroke single cylinder diesel engine using linseed biodiesel and their performance and emission characteristics were studied. Results of performance __ampersandsignamp; emission using linseed biodiesel were compared with that of diesel fuel.