Objective __ampersandsignmdash; To assess the value of c-reactive protein as a diagnostic tool in neonatal septicemia, To find prognostic value of quantitative assay of serial CRP in neonatal septicemia and usefulness of serial CRP for guiding duration of antibiotic therapy in neonatal septicemia. Research Design and Methods MATERIALS AND METHODS A total of 35 full-term neonates of birth weight __ampersandsigngt;2.5 kg admitted in Nursery Balchikitsalaya RNT Medical College, Udaipur (Lodger and intramural) were included and undertook relevant routine investigation to assess the neonatal septicemia i.e. blood was taken for blood culture, blood cell count with differential and quantitative CRP and micro ESR. Results__ampersandsignmdash; C-reactive protein is having __ampersandsigngt;90% sensitivity in diagnosis of neonatal septicemia as compared with other parameters of sepsis screening. A serial decline in CRP value has a strong positive correlation with duration of antibiotic therapy. (P __ampersandsignlt; 0.001) Conclusions- C-reactive protein is highly sensitive test in diagnosis of neonatal septicemia.CRP estimation should also be done on day-4 i.e. after 72 hours of starting antibiotic therapy in a septic newborn. Persistently high values have prognostic implications and antibiotics should be changed if clinically justified. Serial CRP estimation have greater value as compared to single CRP estimation in judging the course and outcome of neonatal septicemia.