The concentrations of Cadmium (Cd), Chromium (Cr), Lead (Pb), Manganese (Mn) and Aluminium (Al) in water, sediments , gills and flesh of both Labio rohita and Edaphodon kewai from River Yamuna in Allahabad, U.P., India, were determined by Atomic Absorption Spectrophotometer. The pattern of distribution of the heavy metals showed a prevalence of Cr and Mn over other metals in the organs of the two fish species as well as in the water and sediment samples. The highest concentration of Mn was in the sediment and low in water. The unexpectedly high concentration value obtained for Cr and Mn calls for medical alertness since it exceeded the WHO recommended acceptable limits for consumption. Al was not detected in the organs of fish species, water and sediment. Cd and Pb distribution in all the samples was lower than the WHO recommended acceptable limits for consumption.