Foramen Spinosum is a foramen in the greater wing of Sphenoid bone transmitting Middle meningeal vessels and Nervous spinosus. In the present study, Foramen Spinosum was studied in Fifty dried human skulls of which 25 were male and 25 female. In each skull, the location and shape of foramen spinosum, it__ampersandsignrsquo;s relation to spine of sphenoid and foramen ovale were noted. Dimensions of the foramen were taken and data was analysed. Absence of foramen was noticed in 1%, confluence of foramen ovale and spinosum in 2%, asymmetry in shape and size were noted and in two skulls, foramen was present lateral to the spine of sphenoid. This knowledge will be useful to Neurosurgeons to identify and preserve the neurovascular structures while approaching middle cranial fossa.