Background: Chronic Suppurative Otitis Media (CSOM) is a common infectious chronic ear disease in India.The present study was aimed to identify bacterial isolates associated with CSOM and their Antibiogram in patients attending ENT OPD of Mahatma Gandhi Medical College __ampersandsignamp; Hospital, Jaipur. Materials and Methods: Samples were taken from 190 patients (both male and female) in all age groups during the period of Jan 2012 to June 2012 suffering from CSOM and having active ear discharge. Their Gram staining, Direct microscopy with KOH, Culture sensitivity, and Biochemical tests were carried out to identify the organisms and to know their sensitivity pattern. Drug susceptibility testing was conducted using a modified Kirby Bauer disk diffusion method. Results: The most common causal organisms isolated were Pseudomonas aeruginosa 80 (45.9%) followed by Staphylococcus aureus 46 (26.4%) amongst the 167 (87.9%) bacterial isolates (including 10 isolates of MRSA). Fungi accounted for 7 (3.7%) of the isolates while 16 (8.4%) were culture negative isolates. The antimicrobial profile of the major isolates i.e. Pseudomonas and Staph. Aureus revealed maximum sensitivity to Piperacillin / Tazobactum against 90% isolates. Conclusion: Pseudomonas aeruginosa is the most common isolate followed by Staphylococcus aureus. Both of these are sensitive to Piperacillin / Tazobactum. The study of microbial pattern and their antibiotic sensitivity determines the prevalent bacterial organisms causing CSOM in local area and to start empirical and more targeted treatment of otitis media and its complications for successful outcome, thus to prevent the emergence of resistant strains.