Background: Accurate knowledge of coronary ostia is not only important for anatomists but also for radiologists and intervention cardiologists performing angiographies and shunt surgeries. The high prevalence, morbidity, mortality and enormous socioeconomic burden of coronary artery disease, has necessitated us to take up this study. We have made a sincere attempt to describe the normal and variant anatomy of the coronary ostia in 64 cadaveric hearts of Indian origin. The origin of coronary arteries, the number of coronary ostia, their location in relation to sinotubular junction and the diameter of coronary ostia were observed and their clinical implications are discussed. Aim: To study the variations in number, location of coronary ostia in aortic sinuses and measuring their diameter. Methodology: Sixty four cadaveric hearts with great vessels attached were analyzed in this study. The ascending aorta cut longitudinally at the posterior aortic sinus to visualize right and left posterior aortic sinus for analysis. Results: In all 64 heart specimens right coronary ostia was present in anterior aortic sinus and left coronary ostia in left posterior aortic sinus. Multiple coronary ostia were noted in 18.7% of cases. Conclusion: The great importance of coronary catheterization for diagnostic and therapeutic purposes has currently motivated the present study.