Seasonality in reproductive cycles as well as testicular and ovarian biochemical changes is very common among teleost. Seasonal gonadal biochemical changes have potent effect on gonadal maturation and period of spawning. Determination of seasonal variation in percentage value of lipids, protein and water in gonadal tissue and establish the possible correlation with gonadal maturity was the objective of the study. Our study of Gonado-somatic index (GSI) reveals that Monsoon was the spawning season of fish. Gonadal tissue biochemical study showed that the lipid percentage was highest during resting phase of testis and spawning phase of ovary, while lowest during resting phase of ovary and spawning phase of testis. We also found that the monthly variation of lipid concentration in ovary was 1.79 times higher than that of the testis. Protein percentage was highest during spawning phase and lowest during resting phase in both gonads. Monthly variation of protein content in the testis and the ovary was almost equal in percentage. Water percentage was lowest during resting phase of testis and spawning phase of ovary, while highest during resting phase of ovary and spawning phase of testis, water concentration in ovary was 1.10 times higher than testis. Finally Gonado-somatic index correlate with gonadal lipid, protein and water percentage, this study show that the water content (R2 = 0.664) is more responsible for maturation of testis and lipid content (R2 = 0.601) for ovary, as compare to other factors.