IJCRRI Journ Cur Res ReInternational Journal of Current Research and ReviewI Journ Cur Res Re2231-21960975-5241Radiance Research Academy1092General SciencesBIODEGRADATION OF REACTIVE RED 2 AZO DYE BY BACILLUS LICHENIFORMIS ISOLATED FROM TEXTILE EFFLUENT CONTAMINATED SITE SudhaD.BalagurunathanR.25092013)19This article is copyright of Popeye Publishing, 20092009This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.

A bacterial isolate Bacillus licheniformis was able to degrade reactive red 2 dye, optimally at pH 9, temperature at 37__ampersandsigndeg;C, dye concentration of 50 mg/l at 20% inoculums size. Glucose, NH4NO3 were found to be the best additional carbon and nitrogen sources. The extracellular enzyme from Bacillus licheniformis was studied for dye decolourization potential. Biodegradation was confirmed by analyzing the product using thin layer chromatography (TLC) and gas chromotograpy- mass spectrometry (GC-MS). GC-MS analysis indicated the formation of 2, 4-dichloro-6-[(1H-indazol-5-ylimino)-methyl]-phenol, benzene sulfonamide, 1H indole and urea as final metabolites formed by Bacillus licheniformis. These results indicate the high potential of Bacillus licheniformis to serve as an excellent biomass for the use in reactive red 2 dye removal.

Reactive red 2 Bacillus licheniformis lignin peroxidase Biodegradation and Gas chromotograpy- Mass spectrometry.