Objectives: Osteomyelitis has been continuing as the most important cause of morbidity among patients with bone infections. Even though early detection of cases and advanced treatments are in process, osteomyelitis is still continued as a major problem due to treatment failures and multidrug resistance. This study was conducted to determine the bacteriological profile of osteomyelitis and their susceptibility pattern to various antimicrobial drugs. The information would guide clinicians in treating osteomyelitis at the initial level so that chronic osteomyelitis can be prevented. Materials and Methods: A total of 100 clinically diagnosed cases of osteomyelitis were included in the study. Clinical specimens like pus, pus swabs, sequestrum of bone and synovial fluid were taken and cultured aerobically. The organisms isolated were identified by routine standard operative procedures. Antimicrobial susceptibility testing was done by Modified Kirby-Bauer__ampersandsignrsquo;s disc diffusion method and the results were interpreted following CLSI guidelines. Methicillin resistance was screened by using Oxacillin disks (1 mcg). Statistical analysis used: Data obtained was presented in counts and percentages and analysed with Fisher__ampersandsignrsquo;s Exact Probability test as applicable. Results: The predominant organisms isolated were Staphylococcus aureus (53.48%) followed by Staphylococcus epidermidis (13.95%), Pseudomonas areuginosa (10.46%), Proteus mirabilis (9.30%), Acinetobacter anitratus (6.97%), Klebsiella pneumoniae (5.82%). Cultures were sterile in 14 % of the cases. Among the isolates of Staphylococcus aureus, 30.33%. were methicillin resistant (MRSA). Most of the Gram positive bacteria were susceptible to Vancomycin, Levofloxacin, Piperacillin/ Tazobactam and Imipenem whereas Gram negative bacteria were susceptible to Piperacillin / Tazobactam, Imipenem, Levofloxacin, Amikacin and Tobramycin. All Methicillin resistant staphylococcus aureus (MRSA) strains were sensitive to Vancomycin. Conclusion: Emerging multidrug resistant strains is a major concern to treat Osteomyelitis. Appropriate selection of antibiotic would help to treat the disease successfully and limit the emergence of drug resistant strains to prevent morbidity and mortality.