Background: Literature reveals that Periodontal disease could be a risk factor for cardio vascular disease. Available meta-analyses find significant heterogeneity which lead to the need for further observational and interventional studies. Host response to periodontal disease include the production of number of enzymes that are released by stromal, epithelial or inflammatory cells. They are associated with either cell injury or cell death. Lactate dehydrogenase [LDH] is the one among those enzymes and it is an intracellular enzyme released by the inflammatory cells during tissue destructive phases. Metabolic changes in the periodontium could be regulated by these enzymatic changes. LDH enzyme activities are higher in chronic periodontitis and in myocardial infarction. Estimation of the iso-enzymes of lactate dehydrogenase in serum is a well-established laboratory procedure for helping diagnose acute myocardial infarction. Objective: The present study was designed to compare the levels of LDH in saliva of the patients with Acute Myocardial Infarction with Chronic Peridontitis, [G-A], Systemically healthy with Chronic Periodontitis [G-B], and Systemically and Periodontally healthy subjects [G-C]. Materials and methods: A total of 30 subiects 10 AMI with Chronic Periodontitis, and 10 Systemically healthy with Chronic Periodontitis and 10 Systemically and Periodontally healthy were recruited. Clinical Periodontal measurements were recorded and stimulated whole saliva was collected. The patients with AMI were examined after 3-4 days after admission to the coronary care unit. The activities of LDH enzyme levels were measured spectrometrically after centrifuging the collected salivary samples, by the optimized kinetic method of deutsche Gesselschaft Fur Klinische Cheime [DCKC]. RESULTS: The saliva of patients with AMI and Periodontitis had significantly higher LDH enzyme activities.Similarly the saliva of patients with Chronic Periodontitis also have higher levels of LDH enzyme activities. LDH enzyme activities correlated significantly in both test groups. LDH enzyme levels are significantly lower in saliva of Systemically and Periodontally healthy patients. CONCLUSION: The present study showed higher levels of LDH in both Acute myocardial infarction [AMI] patients and Chronic Periodontitis [CP] patients. This could prove strong association between the two diseases. Number of samples in this study was less which could be a limitation. Further longitudinal studies with larger number of samples could confirm the association. Hence this estimation of LDH levels in saliva could be an non invasive method to detect acute myocardial infarction [AMI] and chronic periodontitis [CP].