Radiance Research AcademyInternational Journal of Current Research and Review2231-21960975-52411417EnglishN2022September6Healthcare Formulation and Evaluation of Euterpeoleracea In-Situ Gel for Better Treatment of Conjunctivitis       English0106Kanuri SpandanaEnglish Ramineni Shireesh KiranEnglish Ganti Subrahmanya SharmaEnglish Tadikonda Rama RaoEnglish Introduction: This study is to formulate the Euterpe Oleracea in-situ gel for better treatment of conjunctivitis. The Euterpe Oleracea ophthalmic drug solution can treat the ocular infections effectively. Aim: Formulation and evaluation of Eutepe Oleracea in-situ gel for better treatment of conjunctivitis Methodology: Euterpe Oleracea drug containing in-situ gel prepared by using different polymers like carbopol-940, HPMC K15, HPMC K100, HPMC K4M, as a pH triggered gelling system, these polymers are used to controlled release to increase bioavailability. Result: After the formulation prepared it will undergo evaluation i.e. visual appearance, pH, clarity checking, gel capacity, drug content and rheological studies, in-vitro drug release studies, sterility testing were determined. The prepared in situ gel was clear and transparent, it is having good gelling capacity. Conclusion: The drug content of these formulations was determined to be 98%. Developed formulation was stable, and non-irritant, effective therapeutically it can be release in the form of sustained release. EnglishEuterpe Oleracea, HPMC, Citric acid, Disodium hydrogen phosphate, Tween-80, Benzalkonium chloridehttp://ijcrr.com/abstract.php?article_id=4591http://ijcrr.com/article_html.php?did=4591 1. Saini R. In-situ gels- A New Trends in ophthalmic drug delivery system. IJPSR,2015:6(05);886-890. 2. Dol H. Formulation and evaluation of In-situ Ophthalmic gels of Moxifloxacin Hydrochloride. The Pharma Innov J. 2014;3(5):60-66. 3. Agarwal K. In-situ gel formation for ocular drug delivery system, Asian J Bio Pharm sci. 2011;1(4):1-7. 4. Rathore K.S. ‘’In situ gelling ophthalmic drug delivery system: An overview’’, Int J Pharm sci;2010,Vol,2, supl 4,30-34. 5. Mali N.M, Hajare A.A., In-situ gel forming system for sustained ocular drug delivery. Eur industrial pharmacy. 2010;5, 17-20. 6. Wagh VD. Polymers used in ocular dosage form and drug delivery system. Asian J Pharm 2008;2(1) 7. Jain SP. In-situ ophthalmic gel for Ciprofloxacin Hydrochloride for once a day sustained delivery, drug development Ind Pharm, 2008, 34, 445-452. 8. Basavaraj K. In-situ -forming hydrogels for sustained ophthalmic drug delivery 2007;122:119. 9. Doijad RC. Sustained ophthalmic delivery of gatifloxacin from in situ gelling system, Indian J Pharma sci, 2006, 68,814-818. 10. Gaudana R. Recent perspectives in ocular drug delivery, Pharmaceutical Research, 2009; vol.26, no.5, 1197-1216. 11. Chrysanthi L, Skevaki. Treatment of viral conjunctivitis with antiviral drugs. Drugs,2011; 71(3): 331-347. 12. Pacheco-palencia LA, Talcott ST. Chemical stability of acai fruit anthocyanins as influenced by naturally occurring and externally added polyphenolic cofactors in model systems, food chemistry, 2010; 118(1):17-25. 13. Kang J. Flavonoids from acai pulp and their antioxidant and anti- inflammatory activities. Food chem, 2011; 128(1): 152-157. 14. Mahesh N, Hajare A, Ion activated in situ gel system for ophthalmic delivery of Moxifloxacin hydrochloride. Lat. Am. J. Pharm 2010; 29(6), 876-882. 15. Mudgil M. Nanotechnology: a new approach for ocular drug delivery system, IJPPS 2012; vol.4, no.2,pp. 105-112. 16. Tangri P, Khurana S, Basics of ocular drug delivery system, Int J Res Pharm Biomed Sci , 2011; vol.2, no.4, pp.1541-1552. 17. Venkatesh N.P, Liladhar, In situ gel based drug delivery system. 2011; Current drug therapy,6(3),213-222. 18. Jain SP. In situ ophthalmic gel of ciprofloxacin hydrochloride for once a day sustained delivery. Dug development and industrial pharmacy,2008; vol.34. 19. Brittain HG. Analytical profiles of drug substances and excipients. Elsevier 23: 321-361. 20. Rathi V.M, Murthy S.I. Allergic conjunctivitis. Community eye Health,2017. 30(99),7-10. 21. Shen J, Gan L, Zhu C. Novel NSAIDs ophthalmic formulation: Flubiprofen axetil emulsion with low irritancy and improved anti-inflammation effect, Int. J. Pharm, 2008; vol.361, no.1-2, pp. 222-229. 22. Amir A. Azari MD, Arabi A, MD, Conjunctivitis: A systemic review, Vision Research is J Ophthalmic. 2000; 15(3):372-395. 23. Vini R. Ocular Camouflage adoption for an Anophthalmic Anthrophobic Patient, IJCRR - Vol 13 Issue 10, May, 2021; page no. 11-15. 24. Sahane NK, Banarjee SK. Ocular Inserts: A Review, IJCRR – Vol 02 Issue 01, January, 2010, page no. 03-16.

Radiance Research AcademyInternational Journal of Current Research and Review2231-21960975-52411417EnglishN2022September6Healthcare A Case Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and Co-Amoxicillin in Patients with Chronic Suppurative Otitis Media       English0710Shafaque MehboobEnglish Darakhshan M. SaleemEnglish Hurtamina KhanEnglish Humaira SiddiqueEnglish Naveed AhmedEnglish Muslim BashirEnglish Introduction: Chronic suppurative otitis media (CSOM) is middle ear infection, perforation and inflammation usually with purulent middle ear discharge. Different immune markers such as IgE and interferon were proved to be elevated in CSOM. Aim/Objective: The aim of the study is to evaluate of these inflammatory biomarkers (IL6, IL8 and TNF gamma) in patient of CSOM to suggest future therapy of chronic infections with inflammations. Methodology: This is a case-control study conducted in ENT ward in a tertiary care hospital in Jinnah Post Medical centre, Karachi during November 2017 to May 2018. Patients or healthy volunteers were divided into two groups as G1 (without any disease) and G2 (CSOM patients without treatment), G3 (CSOM patients treated with co-amoxicillin) and G4 (CSOM patients treated with ciprofloxacin). The serum was subjected for the quantitative measurement of IL6, IL8 and TNF Gamma by ELISA Kit method via antibody-antigen-enzyme complex formation and measured at 450 nm. Result: Study shows that IL6, IL8 and TNF gamma concentration was significantly high in G2 (positive control), G3 (ciprofloxacin) and G4 (co-amoxicillin) in contrast with G1 (negative control). Moreover, IL6 concentration was significantly low in G3 (ciprofloxacin) and G4 (co-amoxicillin) as compare to G2 (positive control). Conclusion: Elevated levels of IL6, IL8 and TNF gamma in CSOM showed alteration of immune response in chronic suppurative otitis media. Moreover, anti-inflammatory role of ciprofloxacin and co-amoxicillin in CSOM may provide additional benefit beside attenuation of infection. EnglishChronic suppurative otitis media, Ciprofloxacin, Co-amoxicillin, Immune markers, ELISA Kit method, Anti-inflammatory rolehttp://ijcrr.com/abstract.php?article_id=4592http://ijcrr.com/article_html.php?did=4592 1. Haynes DS, Rutka J, Hawke M, Roland PS. Ototoxicity of ototopical drops--an update. Otolaryngol Clin North Am. 2007; 40:669. 2. Mehboob S, Rafi ST, Mehjabeen HK. Serum Immunoglobulin E and Serotonin levels in Chronic Supporative Otitis Media Patients with and without treatment. Pak J of Med Sci. 2021 Sep;37(5):1414. 3. Del Valle DM, Kim-Schulze S, Huang HH, Beckmann ND, Nirenberg S, Wang B, et al. An inflammatory cytokine signature predicts COVID-19 severity and survival. Nature medicine. 2020 Oct;26(10):1636-43. 4. Jafarzadeh A, Nemati M, Jafarzadeh S. Contribution of STAT3 to the pathogenesis of COVID-19. Microbial Pathogenesis. 2021 Mar 7:104836. 5. Brat DJ, Bellail AC, Van Meir EG. “The role of interleukin-8 and its receptors in glioma genesis and tumoral angiogenesis”. Neuro-oncology. 2005;7 (2): 122–133. doi:10.1215/ s1152851704001061. PMC 1871893. 6. Spahn TW, Eugster HP, Fontana A, Domschke W, Kucharzik T. Role of lymphotoxin in experimental models of infectious diseases: potential benefits and risks of a therapeutic inhibition of the lymphotoxin-β receptor pathway. Infection and immunity. 2005; 1:73(11):7077-88. 7. Acuin J. Geneva: World Health Organization; Global burden of disease due to chronic suppurative otitis media. Disease, deafness, deaths and DALYs Chronic Suppurative Otitis Media– Burden of Illness and Management Options. 2004; 9–23. 8. Mehboob S, Rafi SMT, Ahmed N, Mehjabeen M. Association of hearing loss with depression, anxiety and stress in patients suffering from Chronic Suppurative Otitis Media. Pak J Med Sci. 2019;35(2):510-514. doi: https://doi.org/10.12669/ pjms.35.2.152. 9. Gogos CA, Skoutelis, A, Lekkou, A, Drosou E, Starakis I, Marangos MN, et al. Comparative effects of ciprofloxacin and ceftazidime on cytokine production in patients with severe sepsis caused by gram-negative bacteria. Antimicrobial agents and chemotherapy. 2004; 48(8),2793-2798. 10. Demartini G, Esposti D, Marthyn P, Lapidari A, Fraschini F, Scaglione F. Effect of multiple doses of clarithromycin and amoxicillin on IL-6, IFNγ and IL-10 plasma levels in patients with community acquired pneumonia. Journal of chemotherapy. 2004; 1:16(1):82-5.

Radiance Research AcademyInternational Journal of Current Research and Review2231-21960975-52411417EnglishN2022September6Healthcare Association of Hyperlipidemia in Second Trimester of Pregnancy with Maternal and Neonatal Outcomes     English1114Shivani AgarwalEnglish Tanu SharmaEnglish Introduction: Pregnancy is a state of metabolic stress associated with high lipid levels; the highest concentration observed during third trimester. The imbalance in the amount of lipidshas been related to maternal-perinatal pathologies such as preeclampsia (PE), gestational diabetes mellitus (GDM) and preterm birth. Aim/Objectives: Our study was aimed at studying the association of serum lipid profile during second trimester with the various adverse pregnancy outcomes. Methods: A cohort of 150 antenatal mothers were recruited and were evaluated for serum lipid profile during the second trimester. They were followed up till delivery and maternal and neonatal outcome, were recorded. Conclusion: It was concluded that there were direct implications of dyslipidemia on maternal and perinatal outcomes. Increase in maternal TG, TC, LDL and VLDL concentrations were the most important changes affecting maternal outcomes and had significant association with GDM, PE and PTB. HDL did not have any association with any adverse maternal or neonatal outcome. EnglishPerinatal, Pre-eclampsia (PE), Gestational diabetes mellitus (GDM), Preterm birth (PTB), Adverse pregnancy outcomes, Perinatal pathologies, Dyslipidemiahttp://ijcrr.com/abstract.php?article_id=4593http://ijcrr.com/article_html.php?did=4593 1. Monika M. Dyslipidemia in Pregnancy. Am college of Card. 2014. 2. Grimes SB, Wild R. Effect of Pregnancy on Lipid Metabolism and Lipoprotein Levels. 2018 Feb 20. 3. Wild R, Weedin EA, GillE. A women health consideration for lipid management, EndocrineMetab Clin North Am.2016;45(1):65-85 4. Mc Gladdery SH,Frolich JJ.Lipoprotein lipase and ApoEpolymorphisms: relationships to hypertriglyceridemia during pregnancy. J Lipid Res 2001;42(11):1905-12. 5. Jin W-Y, Lin S-L, Hou R-L, Chen X-Y, Han T, Jin H et al. Association between maternal lipid profile and pregnancy complications and perinatal outcomes: a population-based study from China; BMC pregnancy and childbirth (2016)16;60. 6. Ghodke B, Puskuru R, Mehta V. Association of lipid profile in pregnancy with Preeclampsia, Gestational diabetes mellitusand preterm delivery.2017; Cureus 9 (7): e 1420. 7. Singh A, Kujur A, Jain P.Fetomaternal impact of altered lipid profile in pregnancy. Int J Reprod Contracept Obstet Gynecol. 2018 Jan ;7(1):132-136. 8. Sharami S, Ranjbar ZA, Alizadeh F, Kazemnejad E. The relationship of hyperlipidemia with maternal and neonatal outcomes in pregnancy: A cross sectional study. Int J Reprod Biomed 2019; 17:739-748. 9. Yuan li, Xiaoqian W, Fengjuan J, Winqing C, Jie L, Chen X. Serum lipid levels in relation to clinical outcomes in pregnant women with gestational diabetes mellitus: An observational cohort study. BMC(2021) 20:125.