IJCRR - Vol 06 Issue 12, June, 2014
Date of Publication: 23-Jun-2014
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LIQUID BASED CYTOLOGY- IS IT A GOOD ALTERNATIVE?
Author: Sonti Sulochana, Divya Gopalan, Chitra Srinivasan
Abstract:The objectives of the study were to evaluate Liquid Based Cytology (LBC) over conventional Pap smear with respect to adequacy of smear, preservation of morphological features, clarity of background, detection of infective organisms and dysplastic cells. Methodology: the samples were collected at the OBG clinic using the cytobrush, and Pap staining and analysis was done in the Department of Pathology. The sample size was 114. Under speculum examination, the cytobrush was introduced into the cervical os and scraped. The material was smeared onto a slide to prepare a conventional Pap smear. The brush was then dropped into a vial containing the preservative. This sample was then subjected to processing which dispersed the sample to separate the representative cells and unwanted cell debris. A smear was then prepared using the required cells. Both smears were stained by the routine Pap stain and examined under a microscope. Their findings were recorded and the differences were analyzed. Results: There was not much difference in the sensitivity between the conventional Pap smear and LBC in detecting infective organisms. However dysplastic changes were detected in two smears using LBC whereas this was not possible using the conventional smear. Conclusion: Using LBC it was possible to detect infective organisms even when their load was low. Since the cells are in a monolayer, and the smear is uniformly prepared, the quality of the smear is improved thereby decreasing the screening time and easier to read. Therefore LBC can be considered superior to conventional smear with respect to adequacy of smear, preservation of morphological features, clarity of background, detection of infective organisms like bacterial vaginosis, trichomonas vaginalis, Candida etc and dysplastic cells.
Keywords: LBC, Pap smear, bacterial vaginosis, trichomonas vaginalis, Candida
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