International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, Google Scholar, Open J-Gate, ROAD, Indian Citation Index (ICI), ResearchGATE, Ulrich's Periodicals Directory, WorldCat (World's largest network of library content and services)

Search Articles

Track manuscript

Full Html

IJCRR - 13(19), October, 2021

Pages: 58-64

Date of Publication: 11-Oct-2021

Print Article   Download XML  Download PDF

Tolerance and Remediation Potential of Water Microbes against Nitrate

Author: Thakur Preeti, Gauba Pammi

Category: Healthcare

Abstract:Introduction: Nitrate is the major concern of groundwater. High concentration arises due to the massive application of chemical fertilizers. It causes various issues Blue Baby Syndrome, respiratory problems etc. Conventional methods are available to remove nitrate, but they can sequester the nitrate and not treat it. Bioremediation is a low-cost technique and is efficient in nitrate removal. Objective: Isolation and identification of nitrate-reducing microbes from the polluted site of Yamuna river. Methods: This paper focuses on microbe's isolationusingBioremediation technique and their identification through Molecular and Biochemical tests. Result: 4 microbes were found to have the potential for remediating nitrate. Microbes were inoculated in nitrate broth and incubated (37?C) under both aerobic and anaerobic conditions. Phenotypical and molecular characterization was done. The consensus sequence was submitted in GenBank and accession numbers were obtained.These were Enterobacteraerogenes (MN252552), Lelliotiaamnigena (MN647560), E. coli K12 (MN754025) and Klebsiella oxytoca (MT457847) respectively. After incubation, these strains showed 48.4%, 34.1%, 42.90% and 33.66% of nitrate remediation under anaerobic conditions while almost negligible under aerobic. Further, these microbes were inoculated in broth containing 1500mg/l nitrate equivalent to the maximum reported nitrate in Yamuna River. E. coli and Enterobacter aerogenes remediated 44% and 46% of 1500mg/l nitrate respectively. Conclusion: These two microbes were found to have moderate potential to remediate nitrate (1500mg/l) which brings novelty to this study. A further detailed study on using microbial consortium to enhance the capability of nitrate remediation will be useful.

Keywords: Nitrate, Fertilizers, Blue Baby Syndrome, Mutation, Bioremediation, Consortium

Full Text:


Nitrogen is one of the essential nutrients required by plants for their physiological activity, but its availability is limited.1 Plants uptake nitrogen from the soil in the form of nitrate (NO3-) and ammonium ion (NH4+).2 Therefore, chemical fertilizers (nitrogenous fertilizers) are required to boost the fertility of soil 3 and its sufficient amount needed to grow food for the increasing human population.4The use of chemical fertilizers has increased by 80% from 1960 to 2000.5 This overuse of nitrogenous fertilizers has a hazardous effect on the nitrogen cycle, and this also has degraded water quality around the world.6 Ammonium nitrate, ammonium sulfate and urea are the commonly used nitrogenous fertilizers. Ammonium (NH4+) is a positively charged ion that is quickly absorbed by soil and with the help of soil microflora, converts ammonia into nitrate (nitrification).7 Nitrate (NO3-) is a negatively charged ion and its highly soluble property makes it mobile as it easily mixes with groundwater.8

                                There are various detrimental effects of nitrate load in drinking water like- Methemoglobinemia in infants commonly known as Blue Baby Syndrome7, Non-Hodgkin Lymphoma,9 respiratory problems, birth defects, tumour in children10, genetic mutation etc. Besides these, the presence of nitrogenous compounds in water leads to eutrophication which is an anaerobic condition responsible for the death of aquatic animals and plants. In wake of these problems, World Health Organization (WHO) set a maximum limit of nitrate in public drinking water as 50mg/l nitrate or 11.3mg/l as nitrate-nitrogen.11The permissible limit of nitrate is 50mg/l for drinking water and no relaxation for the maximum limit. The maximum reported concentration of nitrate in groundwater, Delhi is 1500mg/l.12 Various conventional technologies available to remove nitrate are ion exchange resins, electrodialysis, distillation and reverse osmosis.13 These can remove nitrate, but they produce secondary wastes. Thus, the worthwhile alternative for the denitrification process lies in bioremediation as it is cost-effective and safe.14

         Several denitrifying microbes have been reported to remediate nitrates such as E. coli, Enterobacter aerogenes, Shigella sonnei, Klebsiella pneumonia and Enterobacter amnigenusetc. These all belong to the Enterobacteriaceae family which are Gram-negative and rod-shaped(bacilli). According to the reported data mostly nitrate-reducing microbes are bacilli in nature.15E.coli and Enterobacter amnigenus16have the potential to remediate nitrate. The current study aims to isolate and identify such microbes from the Okhla barrage which is one of the most polluted sites of the Yamuna river.17

Materials and Methods

Water quality assessment

The physical parameters were visually analysed while chemical parameters were analysed by using a water testing kit (CPCB water testing kit) to examine the water quality of the Yamuna river. Physical parameters checked were colour, odour, turbidity, and pH, and chemical parameters evaluated were Alkalinity, Hardness, TDS, Chloride, nitrates, phosphates, ammonia, etc.

Chemicals:  Potassium Nitrate (96% used for nitrate standard) and sulphuric acid (95%) from Qualigens. Salicylic acid (99%) and Nitrate broth from CDH and Himedia respectively. Nutrient broth from CDH. Pure water used was from the Milli Q Biocel unit (Millipore SAS, Malsheim, France).

 Nitrate broth containing Peptone-5g, Beef extract-3g, Potassium nitrate-1g/l, pH(7.0). Nutrient broth containing the following: Peptic digest of animal tissue-5g, Beef extract-1.50g, Yeast extract-1.50g, NaCl-5g/l, pH(7.4).

Collection and isolation of bacterial species

Water samples were collected from the polluted site of Okhla barrage reported as one of the most polluted sites of Yamuna River (CPCB 2017)18 (Table I). Water samples were serially diluted and transferred to nutrient agar containing 500mg/l nitrate and incubated at 37°C for 24hrs. After incubation, bacterial colonies were picked up and then sub-cultured for pure culture isolation.

Standard graph for nitrate

 3.6g of KNO3 dried at 105°C for 24hrs. It was then weighed and dissolved in 500ml of deionized water and labelled as a 1000mg/L nitrate-nitrogen solution. Nitrate-nitrogen into nitrate and nitrate into nitrate-nitrogen vice versa calculations were done using the formula:

Nitrate = Nitrate-nitrogen ×4.43

                                   Nitrate-nitrogen= Nitrate ×0.226

Isolation of bacteria and estimating its nitrate remediation potential

Four different isolates showed tolerance up to 500mg/l nitrate concentration. These isolates were further checked for their nitrate remediation potential.19 Each isolate was inoculated (4% inoculum) in Nitrate broth and incubated for 72hrs (3 days) at 120rpm under both aerobic and anaerobic conditions. The culture was centrifuged at 8609RCF (Relative Centrifugal Force) for 10mins.20 40µl supernatants were taken and 200µl salicylic acid (5% salicylic acid in sulphuric acid) was added to it. The mixture was vortexed and incubated in dark for 10mins. The reaction was stopped by the addition of 2ml NaOH (4N NaOH). The absorbance was taken at 420nm after 20 mins of incubation.21 The degree of remediation of nitrate was calculated by using the formula:

Where X0 is the initial amount of nitrate,

  X1 is the amount of nitrate after remediation(72hrs). The final results are calculated with the respect to control samples. (nitrate broth without microorganisms).

                                          To check the remediation rate of isolated microbes whether they are capable to remediate or not 1500mg/l nitrate concentration which is equivalent up to the reported nitrate concentration of Yamuna river.

Phenotypic characterization

These isolates were phenotypically characterized by biochemical tests like Gram stain, colony morphology, nitrate reduction, Indole production, Citrate test, Methyl red test, Voges Proskauer reaction and utilization of carbohydrates (Rita B et al 2009). IMVIC test and eight carbohydrate tests(Glucose, Adonitol, Arabinose, Lactose, Sorbitol, Mannitol, Rhamnose and Sucrose) were analysed using Hi-Media kit (KB001). Metabolic profiling of the microbes helps in identification.

Identification of microbes using 16S rRNA gene sequence

Isolation of Genomic DNA from Bacteria

Each isolated colony was inoculated in 5ml Nitrate Broth and incubated at 37?C at 200rpm in a shaker incubator. 1.5ml bacterial culture was taken in a microcentrifuge tube and centrifuged at 4?C for 10mins at 4000rpm. After centrifugation, the supernatant was removed. Pellet was resuspended in 180µl of lysozyme solution (pH 8.0) followed by incubation at 37?C for 30mins till lysis was observed. Cells were kept at -80?C for 10mins. After that,30µl lysis solution (pH 8.0) was added and incubated in ice for 10mins. An equal volume of Phenol-Chloroform was added (1:1) to separate the DNA from the protein. The tube was centrifuged at 8000rpm for 5mins at 4?C to collect the upper layer.22 Upper layer was taken in another tube and chloroform (500 µl) was added and then again centrifuged. After centrifuging, the upper layer was collected in a new centrifuge tube and 1/10th part of 1M NaCl, 2-2.5 ethanol (absolute ethanol) was added. The tube was kept at -80?C for 30mins and further centrifuged at 10,000rpm for 30mins at 4?C. The supernatant was completely decanted and the DNA pellet was washed with 70% chilled ethanol. Pellet was centrifuged at 8000rpm for 5mins at 4?C then dry pellet and dissolved in 20µl TE buffer.23

Polymerase Chain Reaction

The polymerase chain reaction was used to identify the microbes by isolating bacterial Genomic DNA using Sambrook protocol23,22Universal primer 27F(5`-AGAGTTTGATCCTGGCTCAG-3`) and 1492r (5`- CGGTTACCTTGTTACGACTT-3`) were used in amplification reactions, which were prepared in a total volume of 20µl with the following conditions: -10µl PCR master mix (Genei),0.4µl Primer F, 0.4µl Primer R, 2µl DNA template(50ng/µl) and 7.2µl Nuclease free water (Hyclone) was added. According to thermal profile 95?C 5mins, 95?C 1mins, 58?C 1mins, 72?C 2mins for 30 cycles and followed by 72?C 10mins in Simple Amplifier thermal cycler (Applied Biosystems) and analysed by 1% agarose gel electrophoresis and stained with ethidium bromide under UV light.


Water Quality Assessment

The physical parameters analysed were pH, colour, odour and turbidity (Table II) and the chemical parameters evaluated were Alkalinity, Hardness, chloride, TDS, Fluoride, Iron, Ammonia, Nitrites, Nitrates, Phosphates, Chlorine, BOD and COD. (Table III)

Collection and isolation of bacterial species

The observed concentration of nitrate in water samples collected from the Okhla barrage was found to be between 75-100mg/l.

          Eleven different bacterial colonies (PP1-PP11)were identified initially from water samples collected from the Okhla Barrage site on a low concentration of nitrate (range start from 50mg/l). Four, out of these were tolerant to high concentrations of nitrate (up to 500mg/l). They were PP3, 5,7 & 10. The remediation potential of these strains was then checked.

Remediation potential of Bacterial strains

The four bacterial strains which showed tolerance against nitrate were selected to further check their nitrate remediation potential. They were marked as PP3, PP5, PP7 & PP10. 4% inoculum was inoculated in Nitrate broth and incubated at 37°C for 72hrs. After incubation, the optical density of each bacterial strain was measured at 420nm along with control. The nitrate remediation rate of each bacterial strain was checked. PP3, PP5, PP7 and PP10 showed negligible nitrate remediation under aerobic conditions. PP3,PP5,PP7 and PP10 showed remediation of 34.1%, 42.9%, 33.6% and 48.4% respectively under anaerobic conditions (Graph I). Each microbe was then phenotypically characterized.

                                           Further, these four bacterial strains were inoculated in nutrient broth containing 1500mg/l nitrate and then incubated under the same above conditions to check their remediation rate. It was seen that PP5 and PP10 showed 44% and 46% nitrate remediation rates while the rest of the two PP3 and PP7 did show slight remediation potential.

Phenotypic Characterization                                                                                                                                                                      

All microbes were found to be Gram-negative and rod-shaped bacteria. They belong to the Enterobacteriaceae family based on the results of biochemical tests which are shown in Table IV.

Genotypic characterization of microbes

These isolates were chosen further to identify the genus and species of microbes and also check similarity percentage with strains using BLAST. Identification of isolates was done using phylogenetic analysis of 16S rRNA sequences. Genomic DNA and PCR band of isolates (PP3, PP5, PP7 and PP10) are shown in figure I.

Phylogenetic analysis

The partial 16S rRNA gene sequence of 1399 base pairs of strain PP10 sequence showed 99% similarity with Enterobacter aerogenes (KP764198). 1476 base pair of strain PP3 had 99% similarity with Lelliotiaamnigena and 1409 base pair of PP5 had similarity with E.coli K12. 1398 base pair of PP7 strain had similarity with Klebsiella oxytoca. A Phylogenetic tree was constructed using Clustal W (multiple sequence alignment) and MEGA software. The phylogenetic tree was inferred from 16S rRNA sequences data using neighbour-joining tree are shown in figure II.

Nucleotide Sequence Accession Number

The nucleotide sequence of isolates PP3, PP5, PP7and PP10 were deposited in the GenBank nucleotide sequence database under accession number MN647560, MN754025, MT457847, and MN252552 respectively.

Statistical Analysis

One-way analysis of variance(ANOVA) was used to compare among different groups. Microsoft Excel was used for data presentation. Each experiment was repeated three times in replicates. Mean and standard deviation were calculated and associated probability (P) <0.05 was statistically significant.


Isolated microbes belonged to the Enterobacteriaceae family and were found to have the potential to remediate nitrate under anaerobic conditions. Though, they revealed negligible nitrate remediation under aerobic conditions. In the primary screening, eleven strains were isolated from water samples that have tolerance against nitrate. Out of these, four strains showed moderate nitrate remediation potential. On molecular characterization, these strains were identified as E.coliK12, Enterobacter aerogenes, Lelliotiaamnigena and Klebsiella oxytoca. The novelty in this study is that the isolated strains (i.e., E.coli K12 and Enterobacter aerogenes) can remediate 44% and 46% of 1500mg/l (339mg/l nitrate-nitrogen) nitrate concentration. So, the remediation capability on this nitrate concentration showed by isolates have not been reported until now. Further detailed study of these microbes is required to enhance the nitrate remediation potential by addition of some carbon sources and consortiums like bacterial-bacterial association.


This study was a successful trial in isolating and identifying adequate nitrate-eliminating bacteria from polluted sites. E.coli and Enterobacter have shown ~45-50% remediation rate under anaerobic conditions against maximum reported nitrate concentration of Yamuna river (i.e., 1500mg/l). No microbes have been found effective remediation at this concentration, till now. Hence, they can be used in diminishing the nitrate load from groundwater and surface water. Moreover, focus on optimizing the remediation conditions of isolates might lead to great results. Even the association of two or more microbes may impact a lot.

Author’s contribution:-All work regarding the development of methods, optimization, the framework of research preparation, review and editing etc are carried out by the authors.


Both authors contributed to the research work and would like to acknowledge the Jaypee Institute of Information Technology for providing financial support. The authors are grateful to the journal’s authors from where the literature of this paper has been reviewed and discussed. The authors are also thankful to IJCRR editorial board members and IJCRR team reviewers who have helped to bring quality to this manuscript.

Source of Funding

Jaypee Institute of Information Technology, Noida

Conflict of Interest

There are no conflicts of interest.

Figure II: -Neighbor-joining trees showing phylogenetic relationships of 16S rRNA gene sequences to closely related GenBank sequences. These trees were prepared using MEGA software. Green Highlight showing the isolated microbes. (A) Phylogenetic tree of Enterobacteraerogenes (B) Phylogenetic tree of Lelliottiaamnigena (C) Phylogenetic tree of Klebsiella oxytoca (D) Phylogenetic tree of E.coli K12


1. Sharma S, Jha PK, Ranjan MR, Singh UK, Jindal T, Sakram G, et al. Nitrate removal efficiency of bacterial consortium (Pseudomonas sp. KW1 and Bacillus sp. YW4) in synthetic nitrate-rich water. Bioresour. Technol. [Internet]. 2017;157(2):553–63. Available from:

2. Chadwick DR, John F, Pain BF, Chambers BJ, Williams J. Plant uptake of nitrogen from the organic nitrogen fraction of animal manures: A laboratory experiment. J Agric. Sci. 2000;134(2):159–68.

3. Savci S. An Agricultural Pollutant?: Chemical Fertilizer. Int. J. Environ. Sci. Technol 2012;3(1):11–4.

4. Galloway JN, Townsend AR, Erisman JW, Bekunda M, Cai Z, Freney JR, et al. Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions. Science (80- ). 2008;320(5878):889–92.

5. Brender JD, Weyer PJ, Romitti PA, Mohanty BP, Shinde MU, Vuong AM, et al. Prenatal nitrate intake from drinking water and selected birth defects in offspring of participants in the national birth defects prevention study. EnvironHealth Perspect. 2013;121(9):1083–9.

6. Kim HR, Yu S, Oh J, Kim KH, Lee JH, Moniruzzaman M, et al. Nitrate contamination and subsequent hydrogeochemical processes of shallow groundwater in agro-livestock farming districts in South Korea. Agric. Ecosyst Environ [Internet]. 2019;2720:50–61.Available from:

7. The D, Parameters C, Quality OF, Drinking OF, In ACS. OF QUALITY OF DRINKING WATER?; A CASE STUDY in Int. J Curr Res Rev. 2011;03(09):33–6.

8. Lin B Le, Sakoda A, Shibasaki R, Suzuki M. A modelling approach to global nitrate leaching caused by anthropogenic fertilisation. Water Res. 2001;35(8):1961–8.

9. Majumdar D, Gupta N. Nitrate pollution of groundwater and associated human health disorders. Indian J Environ. Health. 2000;42(1):28–39.

10. Knekt, P, Järvinen R, Dich J, Hakulinen T. Risk of colorectal and other gastro?intestinal cancers after exposure to nitrate, nitrite and N?nitroso compounds: a follow?up study. Int  J Cancer. 1999;80(6):852–6.

11. Isibor RA, Aderogbin JA. Assessment of Nitrate and Escherichia Coli Contamination of Shallow Groundwater of Ajakanga and Environs, Ibadan, Southwestern Nigeria. Iconic Res Engg. 2020;3(11):108–18.

12. Kumar L. A study of nitrate contamination in groundwater of Delhi, India. Vol. 10, Asian J Water Envt Poll. 2013;91–4.

13. Ward MH, Jones RR, Brender JD, de Kok TM, Weyer PJ, Nolan BT, et al. Drinking water nitrate and human health: An updated review. Int. J. Environ. Res. Public Health. 2018;15(7):1–31.

14. DebRoy S, Das S, Ghosh S, Banerjee S, Chatterjee D, Bhattacharjee A, et al. Isolation of nitrate and phosphate removing bacteria from various environmental sites. Online J. Biol. Sci. 2012;12(2):62–71.

15. Lin JT, Stewart V. Nitrate assimilation by bacteria. Adv Microb Physiol. 1997;39:1–30.

16. Fazzolari E, Mariotti A, Germon JC. Nitrate reduction to ammonia: A dissimilatory process in Enterobacter amnigenus. Can J Microbiol. 1990;36(11):779–85.

17. Sarkar A, Ali S, Kumar S, Shekhar S, Rao SVN. Groundwater Environment in Delhi, India. Groundwater Environment in Asian Cities: Concepts, Methods and Case Studies. Elsevier Inc.; 2016. 77–108 p.

18.  Sharma S, Jha PK, Ranjan MR, Singh UK, Jindal T. Water Quality Monitoring of Yamuna River by Using GIS-Based Water Quality Index in Delhi, India. Int J Curr Microbiol Appl Sci. 2017;6(2):1249–63.

19. F.A.J. Armstrong. Determination of nitrate in water ultraviolet spectrophotometry. Anal Chem. 1963;35:1292–4.

20. Strong PJ, Burgess JE. Treatment methods for wine-related and distillery wastewaters: A review. Bio Remediat J. 2008;12(2):70–87.

21. Chouhan S, Tuteja U, Flora SJ. Isolation, identification and characterization of fluoride resistant bacteria: possible role in bioremediation. Appl Biochem. Microbiol. 2012;48(1):51–8.

22. Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol. 1989;8(4):151–6.

23. Code C. A Practical Approach to Molecular Biology Course Code?: 07B41BT708.


Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021

Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to for more details.

Best Article Award

A study by Dorothy Ebere Adimora et al. entitled \"Remediation for Effects of Domestic Violence on Psychological well-being, Depression and Suicide among Women During COVID-19 Pandemic: A Cross-cultural Study of Nigeria and Spain\" is awarded Best Article of Vol 14 issue 23
A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22
A study by Saurabh Suvidha entitled \"A Case of Mucoid Degeneration of Uterine Fibroid with Hydrosalphinx and Ovarian Cyst\" is awarded Best article of Vol 14 issue 21
A study by Alice Alice entitled \"Strengthening of Human Milk Banking across South Asian Countries: A Next Step Forward\" is awarded Best article of Vol 14 issue 20
A study by Sathyanarayanan AR et al. entitled \"The on-task Attention of Individuals with Autism Spectrum Disorder-An Eye Tracker Study Using Auticare\" is awarded Best article of Vol 14 issue 19
A study by Gupta P. et al. entitled \"A Short Review on \"A Novel Approach in Fast Dissolving Film & their Evaluation Studies\" is awarded Best Article of Vol 14 issue 18.
A study by Shafaque M. et al. entitled \"A Case-Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and CoAmoxicillin in Patients with Chronic Suppurative Otitis Media\" is awarded Best Article of Vol 14 issue 17
A study by Ali Nawaz et al. entitled \"A Comparative Study of Tubeless versus Standard Percutaneous Nephrolithotomy (PCNL) \? A Randomized Controlled Study\" is awarded Best Article for Vol 14 issue 16.
A study by Singh R. et al. entitled \"A Prospective Study to Find the Association of Astigmatism in Patients of Vernal Keratoconjunctivitis (VKC) in a Tertiary Health Care Centre in India (Vindhya Region MP)\" is awarded Best Article for Vol 14 issue 15
A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award

A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award

RSS feed

Indexed and Abstracted in

Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: To achieve a high standard of publication, we adopt Good Publishing Practices (updated in 2022) which are inspired by guidelines provided by Committee on Publication Ethics (COPE), Open Access Scholarly Publishers Association (OASPA) and International Committee of Medical Journal Editors (ICMJE)

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.


International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal


148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

Copyright © 2023 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions