International Journal of Current Research and Review
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IJCRR - 13(16), August, 2021

Pages: 30-37

Date of Publication: 30-Aug-2021


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Antimicrobial Properties of Three Different Bioactive Compounds of Cassia Species Against S. mutans Serotype C (ATCC 25175). An Invitro Study

Author: Ann Polachirakal Tharakan, Madhura Pawar, Sonal Kale, Noreen Qazi, Rahul Deshpande, Suchita Abhay Gaikwad

Category: Healthcare

Abstract:Introduction: Cassia species (Caesalpinaceae) is a medicinal plant used in traditional Indian medicine for various ailments. In this study, three different bioactive compounds of two medicinal plant species Cassia tora and Cassia fistula were obtained and their antimicrobial properties were compared and evaluated against S.mutans serotype C (ATCC25175) and were conducted as a triple-blind study to identify their effectiveness. Aim: The study aims to evaluate and compare the antimicrobial properties of three different Bioactive Compounds of Cassia species and 0.2% chlorhexidine against S. mutans serotype C (ATCC 25175). Results: All the Bioactive Compounds had good antimicrobial activities based on their zones of inhibition; the highest zone of inhibition in mm was formed by Extract 8 which was Cassia Flower extract having a mean inhibitory zone of 9.93mm +0.76. Stastatistical analysis of the results with Kruskal Wallis and Mann Whitney Post Hoc test proved that at any concentration of Extract 8 the inhibition results are comparable to that of 0.2% chlorhexidine with p=0.05. The results confirmed the antimicrobial potential of the Bioactive Compound of Cassia Flower and hence it can be used as a preventive means for dental caries. Conclusion: The Bioactive Compound of Cassia Flower extract derived from cassia plants are only required in minute quantities as compared to their crude extracts. The study confirmed the antimicrobial potential of the plant at different concentrations can be used as preventive and therapeutic measures in preventive dentistry and due to its reduced potency can be used in children effectively.

Keywords: Bioactive compounds, Chlorhexidine, Cassia, Dental caries, Herbal medicines

Full Text:

INTRODUCTION:

The Global Burden of Disease Study in 2016 estimated that 2.4 billion people suffer due to dental caries of permanent teeth worldwide and an additional 486 million children suffer from caries of primary teeth. 1  Thus, prevention is important as it not only detrimentally impacts the quality of life but also becomes a financial drain due to the need for extensive treatments. Prevention can be achieved by maintaining the ecological balance of the naturally existing cell structures. Zaura et al. in 2014 reported that maintaining the stability of the oral biome is important to prevent  ‘dysbiosis’ and according to the author acute infections of the oral mucosa occur but are rare.

The oral microbiota survives the daily chemo-mechanical insults from either food or oral hygiene practices which results in variations in temperature, pH or symbiotic microbes.  By maintaining the state of dynamic equilibrium within a community of organisms subject to gradual changes an ecological homeostasis can be achieved.

According to He et al., 2011 and Schlafer et al.,   plaque microflorae have a symbiotic relationship with the host, acting as a barrier to opportunistic pathogens and carrying out metabolic processes that benefit the host.3,4  The emerging need is to target pathogenic microorganism thus shifts the focus towards maintaining the ‘holobiont’. 5

            Bowden in 1996 reported that Mutans streptococci, particularly Streptococcus mutans as significant odontopathogens that are  implicated to be highly associated with caries in humans.6  Streptococcus mutans being acidogenic and aciduric drives the microbial ecological shift that leads to dental caries and thus appears in primary tooth of children even under six years of age. 7, 8

Chemotherapeutic antimicrobial agents are known to be lethal  for the normal commensals and cellular structures.  Chlorhexidine is the commonly used potent agent with a proven efficiency which also targets the natural microflora. 9,10  It has a  risk of developing skin injuries, such as skin erythema, burns, blisters discoloration of teeth and xerostomia in the mouth. 11,12 Naturally occurring organic substances have fewer disadvantages compared to synthetically obtained chemical plaque or caries inhibiting agent and are milder for use especially in children and infants.13

Cassia species (Caesalpinaceae) is a medicinal plant used in traditional Indian medicine for various ailments and grows annually in all tropical areas.14  Over 5000 species of cassia flowering plants exist and possesses significant anti-inflammatory, antimicrobial antifungal properties and antioxidant properties. 15,16

Bioactive Compounds are the main active ingredients or a chemical substance which may be found in all parts of the plant and have biologically beneficial effects.17 Thus, the aim of the study was to compare and evaluate the antimicrobial properties of three different Bioactive Compounds of Cassia species and 0.2% chlorhexidine against S. mutans serotype C (ATCC 25175).

METHODS:

This study was approved by the Institutional ethics committee of Dr DY Patil Vidyapeeth Pune, Maharashtra, India (ref: DPU/R&R(D)/32(21)/19) and was conducted as a triple blind study. The aim was to evaluate and compare the antimicrobial properties 3 different bioactive compounds at three concentrations (200mcg, 400mcg, 600mcg) and 0.2% chlorhexidine gluconate against S. mutans serotype C (ATCC 25175).

Extract Preparation: 18

The extracts of cassia species were obtained from the scientist which were from the two medicinal plant species Cassia tora and Cassia fistula from Western Pune Maharashtra, India, shade dried authentication was done by comparing with herbarium specimens preserved in Botanical Survey of India, Pune (Maharashtra). Authentication no of Cassia tora  BSI/WC/Cert/2015/SG01,  Cassia fistula is BSI/WC/Cert/2015/SG02.18  The extracts were then finely pulverized and exactly weighed plant material was utilized to prepare extracts with measured volumes of solvents like ethyl acetate, acetone, ethanol, methanol and distilled water then removed under pressure. Weighed amounts of the extracts marked at Extract 6, Extract 7 and Extract 8 were then collected from the scientist and utilized for the study.

Microbiological assay laboratory processing: 19 

Well diffusion method was used to determine zone of inhibition of 3 bioactive extracts in the concentration of (200µg , 400µg, 600µg) against mutans serotype C (ATCC 25175). All the tests were performed under sterile conditions in triplicate by standard norms and protocols . 0.2% chlorhexidine against S. mutans serotype C (ATCC 25175) was used as positive control. Adequate amount of Mueller Hinton Agar was evenly distributed over the surface of 15 cm diameter petri-dish to a thickness of 5 mm and allowed to solidify under aseptic conditions. Streptococcus mutans serotype C (ATCC 25175) was inoculated with a sterile spreader on the agar medium. Standard wells were made with a cupborer (9.0mm)

            Then 0.2% chlorhexidine gluconate,  bioactive Compounds of cassia species with concentration of 200µg , 400µg, 600µg in 0.5ml DMSO solvent was inserted in separate wells of agar plates inoculated with Streptococcus mutans serotype C (ATCC 25175). The plates were incubated at 37 ± 0.1? C for 24 hours. The same procedure was followed for different concentrations of bioactive compounds. After incubation, the plates were observed for zone of inhibition and measured in millimeters.

RESULTS:

The zones of inhibition in mm was obtained. The results were compared statistically with chlorhexidine in the form of mean and standard deviation. The p values were obtained.  Kruskal Wallis and Mann Whitney Post Hoc test was conducted to analyze the data.

The below tables and graphs projects the mean and standard deviation of inhibition zones at 200µg, 400 µg, 600µg for the three Bioactive compound extracts- Extract 6, Extract 7, Extract 8 and 0.2%chlorhexidine gluconate against S. mutans serotype C (ATCC 25175)  and their comparison.

The Table no.1, Graph no.1 showed the extracts with inhibition zones at 200µg, 400 µg, 600 µg  for the three Bioactive compound extracts- Extract 6, Extract 7, and Extract 8 were compared and evaluated against S. mutans serotype C (ATCC 25175). At 200µg concentration the highest zone inhibition in mm was formed by Extract 8 concentration having mean inhibitory zone of 2.03mm ±0.86. At 400µg concentration the highest zone inhibition in mm was formed by Extract 8 concentration having mean inhibitory zone of 6.57mm±0.81. At 600µg concentration the highest zone inhibition in mm was formed by Extract 8 concentration having mean inhibitory zone of 9.93mm +0.76. At all concentrations extract 8 showed the highest zone of inhibition. For all  concentrations of Extracts 6, 7,and 8  at 200µg, 400µg and 600µg there was a significance of p= 0.027.The mean inhibition zone against S. mutans serotype C (ATCC 25175) formed by 0.2% chlorhexidine which was 16.07±0.30 (Graph 2).

The Table no.2 depicts the Post hoc test of Kruskal Wallis and Mann Whitney presenting differences between the extracts 6, 7 and 8 at concentration of 200µg, 400 µg, 600 µg revealed that a significant difference was present between 200µg and 600µg concentrations with p=0.021. A significantly large inhibition zone was formed against S. mutans serotype C by 600 µg concentration as compared to 200 µg concentration. No significant difference was found between 200 µg and 400 µg concentration as well as 400 µg and 600 µg concentration.

For Extract 7 at 200 µg Post hoc test revealed that a significant difference was present in inhibition zone when compared to 600µg extract the significance was p = 0.022. No significant difference was found between 200 µg and 400 µg concentration as well as 400 µg and 600 ug concentration.

For Extract 8 at 200µg extract, 400µg extract and 600µg Post hoc test revealed that a significant difference was present between 200µg and 600µg with p= 0.022. A significantly large inhibition zone was formed against S. mutans serotype C by 600 µg concentration. No significant difference was found between 200 µg and 400 µg concentration as well as 400ug and 600 ug concentration.

The Table no.3 showed the difference between the inhibition zones of  Extract 6, 7 and 8 at different concentrations 200µg ,400 µg, 600µg and 0.2% chlorhexidine. The mean inhibition zone against S. mutans serotype C (ATCC 25175) formed by 0.2% chlorhexidine which was 16.07±0.30 (Graph 2).

For Extract 6 a significant difference was found between 200µg extract concentration and 0.2%chorhexidine with p=0.046. No significant difference was found between 400ug and chlorhexidine, 600ug and chlorhexidine indicating that the higher concentration of bioactive compounds showed a comparable action as that of chlorhexidine. The difference between 600 µg of Extract 6 and 0.2 % chlorhexidine was 9.53+0.050 with p=0.05.

For Extract 7 at 200µg, 400µg, 600µg and 0.2% chlorhexidine no significant difference was present between any of the extracts and chlorhexidine indicating that at any concentration of Extract 7 the inhibition results are comparable to that of 0.2% chlorhexidine with p=0.05. The difference between 600 µg of Extract 7 and 0.2 % chlorhexidine was 9.43+0.050.

For Extract 8 at 200 µg, 400 µg 600µg and 0.2% chlorhexidine no significant difference was present between any of the extracts and chlorhexidine indicating that at any concentration of Extract 8 the inhibition results are comparable to that of 0.2% chlorhexidine with p=0.05. The difference between 600 µg of Extract 8 and 0.2 % chlorhexidine was only 6.13+0.050.

DISCUSSION:

Current evidence suggests that the cariogenic factors that drive streptococcus mutans to be virulent is its potential to thrive in acidic environment’s, its ability to lower the pH, ferment simple sugars, synthesize sucrose, promote its adhesion and buildup the plaque-ecology especially for those having a low socioeconomic status with limited access to healthcare.20   Milsom et al. described that children with an already existing caries lesion have a 5–6 times higher incidence of developing new caries lesions compared to previously caries-free children. 20

Peterson et al (2014) concluded with  next-generation sequencing (NGS)  that Streptococcus-species was found to be the most abundant genus (>50% of the microorganisms)in the microbial composition of the dental plaque.21 Damle et al (2016) concluded that the number of s mutans colonies increases with increasing age and it is more in children with lesions that can be seen clinically and the amount of S. mutans in the saliva is directly proportional to the colonized surfaces. 22  

In this study chlorhexidine(CHX) was used as the control as it is the most extensively used anti-plaque and anti-bacterial agent and it is highly active against the s. mutans strain. Among the available chemotherapeutic agents, chlorhexidine mouthwash is considered as the “gold-standard” due to its proven efficiency, but it exhibits cytotoxic activity on ectodermal cells causing altered taste sensation and extended used causes xerostomia, brown-staining of teeth and fillings. 23

Therefore, the nature-based alternatives with less side effects would be beneficial than the chemically prepared agents. They also have a two-fold advantage of minimal side-effects and being alcohol-sugar free. Nature-based plant products like Aloe vera, Bloodroot Garlic and Propolis have several advantageous properties anti-microbial, wound healing, anti-helminthic, anti-inflammatory, anti-fungal properties of medicinal plants. 24

In this study Cassia plant species was selected for their anti-inflammatory, hypoglycaemic, antiplasmodial properties. 25 Thus can be used as a  broad-spectrum antibacterial agent that is nature based . It is easily obtainable, in Asia, South Africa, Mexico, China, East Africa and Brazil thus making it cost effective. 26

Bioactive compounds are substances are present within all part of the plant such as flavonoids which includes a huge group of naturally occurring organic compounds. It is found in a large variety of plants including fruits, seeds, grains, tea vegetables, nuts, and wine. 26

            The Microbiological assay of well diffusion method was used to determine zone of inhibition and after incubation at 37ºC for 24 hours as per standard protocols. The diameters of inhibition zones produced by the plant extract were measured in mm.

Statistical analysis of the results with Kruskal Wallis and PostHoc Mann Whitney Test  and concluded that the mean and standard deviation of inhibition zones at 600µg for the three Bioactive compounds extracts- against S. mutans serotype C (ATCC 25175) identified that the highest zone inhibition in mm was formed by Extract 8 concentration having mean inhibitory zone of 9.93mm +0.76 followed by Extract 7 was 6.63 mm+0.30 inhibition zone. The least effective concentration of Bioactive compound of cassia species was found with Extract 6 which formed a small zone of 6.53 mm+0.55.

The three bioactive compounds provided by the scientist were Quercetin (Extract 6), Gallic acid (Extract 7), and Cassia flower (Extract 8). All three bioactive compounds have antimicrobial properties based on their zones of inhibition, but the extract labelled as number 8 which is Cassia flower (Tora and Fistula) showed the highest antimicrobial zone when compared to the three extracts.                               

         Quercetin (Extract 6) was isolated and found from both Cassia tora L as well as Cassia Fistula. 26   Quercetin is the main bioflavonoids known for its anti-inflammatory, anti-hypertensive, vasodilator effects, anti-obesity, anti-hypercholesterolemic and anti-atherosclerotic activities.26 It is a naturally occurring flavonoid pigment present in many fruits and vegetables. It is antioxidant rich and plays an important role in helping the body. It is not only an antioxidant but also has anti-inflammatory, and anticancer activities.27 Quercetin induces apoptosis by inducing the dissociation of Bax from Bcl-xL, activating caspases, and inhibiting phosphorylation. 27 It  is metabolized immediately by enzymes in the epithelial cells and further metabolized by the liver. Thus quercetin has proven effects that benefit the oral health. 27 This study proved that Quercetin has an antibacterial effect with a zone of inhibition of 6.53 mm+0.55. This was compared to the zone of inhibition of 0.2% chlorhexidine which was 16.07mm. The zone of inhibition was also compared to the other extracts and was found to be the least.

Gallic acid (GA) is in the group of hydroxybenzoic acids. 28 Many plant extracts have been found to inhibit the growth of oral bacteria , particularly s.mutans and thus may prevent the formation of dental caries. It is the most popular of trihydroxybenzoic acids.  An important source of gallic acid is also tea containing up to 4.5 g/kg fresh weight in tea leaves it also occurs mostly in certain red fruits, black radish, and onions. Apart from its phytochemical role, gallic acid is also used in tanning, ink dyes, and the manufacture of paper. 28 Effects include antihyperglycemic, antilipid peroxidative, antioxidant antimicrobial, anti-inflammatory, anticancer, cardioprotective, gastroprotective, and neuroprotective effect.29  Gallic acid can inhibit motility, adherence and biofilm formation of  Pseudomonas aeruginosaStaphylococcus aureusStreptococcus mutansChromobacterium violaceum, and Listeria monocytogene. 29 In this study the zone of inhibition of Gallic acid on streptococcus mutans serotype C (ATCC 25175)was evaluated at the 600µg concentration the zone was 6.63 and compared with 0.2% chlorhexidine which was 16.07. The zone of inhibition in mm for Extract 7 (Gallic Acid) was more when compared to  bioactive compound of cassia species Extract 6 (Quercetin) .

Cassia flower also known as the ‘Golden Shower’ is widely grown as a decorative tree for its beautiful bunches of yellow flowers. Cassia plants have bright yellow flowers of characteristic- bell shape drop. 30   Cassia provides dyes and is widely used for its medicinal properties. The plant has antipyretic and analgesic effect.31  It was found that extracts of Cassia fistula flowers contained tannins, flavonoids, and anthraquinones compounds in higher amount.32 Cassia flowers has known antibacterial and antifungal activity. In the present work, the extracts are obtained from Cassia fistula flowers. All the Bioactive Compounds had good antimicrobial  activities based on their zones of inhibition but of all the three;  the extract of  cassia flower showed the highest zone inhibition in mm was formed by Extract 8 (Cassia Flower) concentration having mean inhibitory zone of 9.93mm +0.76. Mann Whitney test revealed that at any concentration of Extract 8 the inhibition results are comparable to that of 0.2% chlorhexidine with p=0.05. The difference between 600 µg of Extract 8 and 0.2 % chlorhexidine was only 6.13+0.050.

 The Cassia flower extract can be an excellent substitute for chlorhexidine which can be can prepared in various forms like mouthwashes, gels, sustained release forms, intracanal medicaments, lollipops etc. The bioactive compound extract of cassia flower has a possibility to be used as new antimicrobials for children and adults as well as for prevention of dental caries. Nowadays, the patients are more aware of the harmful effects of synthetic formulations and prefer a natural herbal alternative that is well tolerated by the body. Thus, these natural herbal formulations with their bioactive compound extract can offer a non- potent resolution with minimal adverse effects to improve the oral health of children.

CONCLUSION:

Nature-based cassia plant species is available in all seasons and easily obtainable in many countries and hence provides a novel therapeutic future for combating oral diseases like caries in especially in children. Based on the findings of the study, it was concluded that: (1)The bioactive components of cassia flower extracts appears promising against Streptococcus mutans serotype c (ATCC 25175) and can be used effectively as a novel compound especially in children due to the nature based non synthetic properties for combating dental caries. (2) Future studies are needed to determine the antimicrobial activity of Cassia flower plant extract against Streptococcus mutans on whole saliva to understand its efficacy against polymicrobial or mixed culture. (3) Lastly toxicological investigations of Cassia plant extract also need to be found out to confirm its safety[A1]  for human trials.

ACKNOWLEDGEMENTS:

We acknowledge the immense help received from the scholars whose articles are cited and included in references of this manuscript. We are also grateful to authors/editors/publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed.

There was No Funding or Financial support and No Conflict of interest.

*Significance at p<0.05

A significant difference is present between 200 and 600µg, with Extract 6

A significant difference is present between 200 and 600µg with Extract 7

A significant difference is present between 200 and 600µg with Extract 8

*Significance at p<0.05

For Extract 6- A significant difference is present in inhibition zone between 200µg and 0.2% Chlorhexidine

For Extract 7- No significant difference is present in inhibition zone between 200µg and 0.2% Chlorhexidine, 400µg and 0.2% Chlorhexidine, 600µg and 0.2% Chlorhexidine

No significant difference is present in inhibition zone between 200µg and 0.2% Chlorhexidine, 400µg and 0.2% Chlorhexidine, 600µg and 0.2% Chlorhexidine.

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A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

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