International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - Vol 13 Issue 07, April, 2021

Pages: 112-117

Date of Publication: 12-Apr-2021


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Rp-Hplc Method for Simultaneous Estimation of Vildagliptin and Metformin in Bulk and Pharmaceutical Formulations

Author: V. Nagalakshmi, G. Srinivas Rao, N. Gayathri Devi, S. Mohan

Category: Healthcare

Abstract:Background: Combination therapy of vildagliptin and metformin provides a comprehensive effect on cardiovascular risk factors in the form of preventing hyperinsulinemia and reducing insulin resistance. Objective: To develop a simple, fast, precise, accurate, sensitive RP-HPLC method for simultaneous estimation of metformin and vildagliptin in pure and tablet dosage forms. Method: The mobile phase, a mixture of acetonitrile, methanol and water (15:60:25v/v) pumped at a flow rate of 1.0 ml/min through the column (C18; 5\?, 4.6 X 250 mm, Hypersil) at 35o C temperature. The mobile phase was degassed before use under vacuum by filtration through a 0.2\? nylon membrane. Concentrations were measured at 278 nm by a UV detector at a sensitivity of 20 µl. Results: The linearity lies between 1-5 \?g/ml for metformin and 1-5 \?g/ml for vildagliptin in the method. The correlation coefficient (r2 ) was found to be 0.982 and 0.998 for Metformin and vildagliptin, the limit of detection and limit of quantification were found to be 0.617and 1.87 \?g/ml for metformin and 0.154 and 0.468 \?g/ml for vildagliptin, respectively. The results of the analysis have been validated statistically by recovery studies as per International Conference on Harmonization guidelines. Conclusion: The method showed good reproducibility and recovery with %RSD < 2. Hence, the method was found to be rapid, specific, precise, and accurate and can be successfully applied for the routine analysis of metformin and vildagliptin in the pure and combined dosage form.

Keywords: Vildagliptin, Metformin hydrochloride, Antidiabetic drugs, RP-HPLC method, Simultaneous Estimation, Method development and validation

Full Text:

INTRODUCTION

Chemically known as 3-[diaminomethylidene]-1, 1-dimethylguanidine HCl, metformin HCl is an oral pharmaceutical product used to treat type 2 diabetes mellitus. Metformin is considered an antihyperglycemic drug because, without inducing hypoglycaemia, it reduces blood glucose concentrations in type II diabetes. Metformin is widely known as an insulin sensitizer that leads to a decrease in insulin resistance and a clinically important decrease in insulin levels from plasma fasting. Modest weight loss is another well-known advantage of this drug. For obese type II diabetes patients, Metformin is the medication of choice.1,2

Chemically, Vildagliptin is known as pyrrolidine-2-carbonitrile, (2S)-1-{2-[(3-hydroxyadamantan-1-yl) amino] acetyl}. Vildagliptin, previously known as LAF237, is a novel oral antidiabetic drug in the drug class of the new dipeptidyl peptidase-4 (DPP-4) inhibitor. Vildagliptin subsequently works by inhibiting glucagon-like peptide-1 (GLP-1) and gastric polypeptide inhibitor (GIP) inactivation by DPP-4.3,4

Literature review reveals that for estimation of metformin and vildagliptin in combination and individual dosage form5-9 and validated with parameters,10-16 various analytical methods such as UV-Vis spectroscopy, HPLC and LCMS / MS methods are available. Without using any buffers, the procedure with new composition was developed in the estimation of these compounds using RP-HPLC methods and also the development and validation of a simple, precise, fast and specific method for the determination of metformin and vildagliptin in pure form and its pharmaceutical dosage form were considered of interest.

MATERIALS AND METHODS

Chromatographic conditions

Column: ODS (4.6×250mm, 5µm, Hypersil)

Mobile phase: ACN: Methanol: Water (15:60:25)

Flow rate: 1ml/min

Column temperature: 35oC

The volume of injection: 20µl

λmax: 278nm

Degradation studies

The forced degradation was carried under acidic, basic, oxidative conditions. Both the drugs were separately exposed to stress conditions. After exposing stress conditions, drugs were diluted to the standard concentration of Metformin (30µg/ml & 160µg/ml) and Vildagliptin (30µg/ml & 160µg/ml). Equal volume of both drugs were mixed and analyzed in the chromatographic conditions. The acid stress condition was carried out using 1N HCl, when mixed with the drug solution and kept for 48hours and then injected into the chromatographic column, degradants evaluated using chromatograms. The alkali hydrolysis was carried out using 1N NaOH, when mixed with the drug solution and kept for 48hours and then injected into the chromatographic column to evaluate the degradedness. Hydrogen peroxide is a strong oxidant when 3% H2O2 was added to the 1000µg/ml drug and kept for 48hours and injected into the chromatographic column to check the degradation.

RESULTS

Validation by Method

The method defined has been validated, including parameters such as suitability of the system, linearity, accuracy, precision, robustness, LOD and LOQ.

System suitability

Results of the system suitability study are summarized in the above table 1. Six consecutive injections of the standard solution showed uniform retention time, theoretical plate count, tailing factor and resolution for both the drugs which indicate a good system for analysis.

Specificity

The analytical peak was evaluated as per the methodology and observed the interference of blank, placebo with the analyte peak was there or not. Metformin and vildagliptin peaks were observed at their respective retention times of analyte peaks in figure 3 and 4.When the blank solution was injected, no peak was found in figure 2. The forced degradation study showed the method was highly specific; the chromatographic peak does not interfere with any other impurities. This proves that excipients do not affect the analytical method. On the other hand, the blank peak did not overlap the drug peak. So the method is highly selective.

Accuracy

Validated the accuracy in this method, accurately quantify metformin and vildagliptin tablets content at 50%, 100%, 150% and performed assay in triplicate. The mean per cent recovery of metformin and vildagliptin at each spike level should be not less than 98% and not more than 102%. Results of the accuracy study are presented in table 2. The measured value was obtained by the recovery test. The spiked amount of both the drugs were compared against the recovery amount.

Precision

The precision of the test method by preparing six test preparations using the product blend by mixing the active ingredient with excipients as per the manufacturing formula was determined. Repeatability of Standard metformin and vildagliptin solution was injected six times and peak areas were measured and metformin &vildagliptin per cent RSD was found to be 0.566 & 1.903. Results are given in table 3.

Linearity

A linear relationship between peak areas versus concentrations was observed for metformin and vildagliptin in the range of 50% to 150% of normal concentration. The correlation coefficient was 0.982 for metformin and0.988 for vildagliptin. This proves that the method is linear in the range of 50% to 150 %. Results are given in Table 4.

Robustness

The results of the robustness of the present method had shown by changes in the flow rate and wavelength did not produce significant changes in analytical results which were presented in table 5. As the changes are not significant we can say that the method is robust.

The Detection Limit

LOD for Hydrochloride of metformin = 0.6177

LOD = 0.1544 for vildagliptin

Limit of Quantification

LOQ for Hydrochloride of metformin = 1.8711

LOQ = 0.4688 for vildagliptin

The LOD and LOQ values indicate that the method developed was sensitive, precise and reliable.

Studies of stability

Comparative studies were performed on drugs called metformin and vildagliptin before and after degradation, and acid, base and oxidative degradation results were reported in Table 6.

Research on Stress Degradation

The properties of stress degradation were analysed using a validated chromatographic method for metformin and vildagliptin. Studies of forced degradation are listed in the table. Reports show that the validated approach effectively isolated and separately classified the degradation products. From the reports, it is very clear that drugs were responsive to acidic environments where there was less degradation.

DISCUSSION

The proposed method for the simultaneous determination of metformin and vildagliptin in pharmaceutical dosage form was found to be precise, selective, rapid and economical.5 The interaction study between the two drugs in the standard solution was carried out by comparing peaks of each drug individually with peaks obtained in drug mixture indicating that the analytes did not interact with each other and data were within the acceptance level of ±2.0%.1 The linearity for detector response was observed in the concentration range of 50 to 150% of test concentration and the correlation coefficient (r) for the calibration curve was found to be 1.0. Per cent recovery was found to be within the range of 98.0 % to 102.0% indicating the accuracy of the method.6 The per cent RSD for the tablet analysis and recovery studied is less than 2 which is indicating a high degree of precision.5 The results of recovery studies were found to be linear in 50 % to 150 % of the final assay concentration range indicating linearity and range of the proposed method. The results of the robustness study indicate that the method is robust and is unaffected by small variations in the wavelength and flow rate trails.7 Hence, it can be concluded that the developed RP-HPLC method is accurate, precise, rapid and selective and can be employed successfully for the estimation of metformin and vildagliptin in bulk and pharmaceutical dosage forms.8 Degradation was not observed in metformin and vildagliptin stressed samples that were subjected to acid hydrolysis and oxidative conditions. However, degradation was observed under base hydrolysis. This indicates that the method is specific and stability-indicating.1

CONCLUSION

In pharmaceutical formulations, the author sheds light on the enhancement of HPLC methods for estimating metformin hydrochloride and vildagliptin. It is usually important to design methods in which a very large number of samples are to be analysed with appropriate precision and accuracy in a very short period. It is possible to obtain qualitative results via the HPLC process and can thus be used in analytical analysis. This is an effective technique that provides good results for validation parameters. This approach explicitly performs well on Metformin hydrochloride and Vildagliptin.

ACKNOWLEDGMENT: The authors thanks to the Department of Chemistry, Ch.S.D.St.Theresa’s College for Women (A), Eluru for providing workspace and the authors are grateful to Icon Pharmaceutical Lab for providing instrumental support.

CONFLICT OF INTEREST: The authors declare that no conflict of interest for this research. No funding support for this research

 

References:

  1. Alaa SA, Soha F, Mohamad MM, Abo T. Simultaneous for the Estimation of Metformin and Empagliflozin in Pharmaceutical Dosage Form by HPLC Method. IOSR J Pharm Bio Sci 2019;14(1):75-80.

  2. Thornberry NA Gallwitz B. Mechanism of action of inhibitors of dipeptidyl-peptidase-4 (DPP-4). Best Pract Res Clin Endocrinol Metab 2009;23(4):479-486.

  3. Ahren B, Landin Olsson, Jansson M. Inhibition of dipeptidyl peptidase-4 reduces glycemia, sustains insulin levels, and reduces glucagon levels in type 2 diabetes. J Clin Endocrinol Metab 2004; 89(5):2078-2084.

  4. Mentlein R, Gallwitz B,Schmidt. Dipeptidyl-peptidase IV hydrolyses gastric inhibitory polypeptide, glucagon-like peptide-1(7-36) amide, peptide histidine methionine and is responsible for their degradation in human serum. Eur J Biochem 1993;214(3):829–835.

  5. Shirode1 AR, Maduskar PD, Deodhar MS, Kadam VJ. RP-HPLC and HPTLC methods for simultaneous estimation of Metformin Hydrochloride and Vildagliptin from bulk and their marketed formulation: Development and Validation. Br J Pharm Sci 2014;4(20):2370-2386.

  6. Abu DW, Hamad M, Mallah E, Abu DA, Awad R, Zakaria Z,  et.al. Method Development and Validation of Vildagliptin and Metformin HCl in pharmaceutical dosage form by Reverse phase-High performance liquid chromatography (RP-HPLC). Int J Pharm Sci Res 2018; 9(7):2965-2972.

  7. Subhakar N, Krishna Reddy V, Ravindranadh Reddy T. Development and Validation of RP-HPLC method for simultaneous determination of Vildagliptin and Metformin in bulk and formulation dosage. Int Res J Pharm Appl Sci 2012;2(3):44-50.

  8. Srinivas Ch, AnilkumarCh, Nagaraju B, Nagajyothi J.  Method development and Validation by RP-HPLC for the simultaneous estimation of Metformin and Vildagliptin in pharmaceutical dosage form. Eur J Pharma Med Res 2017;4(6):01-16.

  9. Priyanka DP, Saurabh D, Pandya S. The aim of this review to focus on a comprehensive update of different analytical methods for determination of oral anti-diabetic drugs for the treatment of type 2 diabetes mellitus (T2DM). World J Pharm Sci 2018;6(1):29-39.

  10. International Conference on Harmonization (ICH); Q2 (R1), Validation of analytical procedures: Text and methodology, Geneva, Switzerland, (2005). 

  11. United state Pharmacopoeia, USP 32, NF27, Page No. 2906, 2007.

  12. Mohammad A, Azim Md, Elkady EF, Fouad A. Development and validation of a reversed phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin. Eur J Chem 2012;3(2):152-155.

  13. Shaikh KA, Patil SD, Devkhile AB. Development and validation of a reversed-phase HPLC method for simultaneous estimation of ambroxol hydrochloride and azithromycin in tablet dosage form. J Pharm Biomed Anal 2008;48(5):1481–1484.

  14. Santhosha B, Ravindranath A, Sundari Ch. Validated method for the simultaneous estimation of Metformin Hydrochloride and VIDA by RP-HPLC in bulk and the pharmaceutical dosage form. Int Res J Pharm App Sci 2012;2(3):22-28.

  15. De AK, Ashokkumar B, Pal B. Development and Validation of Same RP-HPLC Method for Separate Estimation of Theophylline and Doxofylline in Tablet Dosage Forms. J Curr Pharm Res 2012;9(1):55-58.

  16. Devika GS, Sudhakar M.  RP HPLC method for simultaneous estimation of metolazone and ramipril in oral solid dosage form. Int J Pharm Bio Sci 2012;3(4):193–200.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

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SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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