International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
logo
slider
slider
slider
slider
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, Google Scholar, Open J-Gate, ROAD, Indian Citation Index (ICI), ResearchGATE, Ulrich's Periodicals Directory, WorldCat (World's largest network of library content and services)

Search Articles

Track manuscript

Full Html

IJCRR - 13(4), February, 2021

Pages: 76-82

Date of Publication: 16-Feb-2021


Print Article   Download XML  Download PDF

Screening and Production of Lovastatin Producing Endophytic Fungus from Phyllanthus reticulatus using Oyster Mushroom Extract

Author: Senthamarai Manogaran, Kovilloo Packiam Kannan, Kowsalya Rathinasamy, Harish Ranganathan, Govardthni Gurusamy

Category: Healthcare

Abstract:Introduction: Endophytic fungi are most commonly found in plants. They are present inside the plants and supporting plants to survive and produce many bioactive metabolites. Lovastatin is one of the secondary metabolites produced by the endophytic fungi. It is used in the treatment of high blood cholesterol and reduces the risk of cardiovascular diseases. Objective: Screening and Production of Lovastatin Producing Endophytic Fungus from Phyllanthus reticulatus using Oyster Mushroom Extract. Methods: In our study, we have isolated endophytic fungi from the leaves and stem of phyllanthus reticulatus. Biodiversity of the endophytic fungi was calculated. Neurospora crassa bioassay was performed to identify the best producer of lovastatin. Their Lovastatin production potential was investigated with two different mediums such as potato dextrose and the mushroom extract. After completion of the fermentation process, the broth was extracted by a simple distillation method and then analyzed for the presence of Lovastatin using UV-Visible spectrophotometer and HPLC. Results: One of the positive and dominant endophytic fungi, Curvularia sp was isolated and chosen for this study. Its Lovastatin producing potential in PDA and mushroom extract medium was investigated and confirmed. Conclusion: Curvularia sp was identified as a better Lovastatin producer when compared to other endophytic fungi isolated from Phyllanthus reticulatus. From the results of the UV and HPLC, the presence of lovastatin is confirmed.

Keywords: Endophytic fungi, Lovastatin, Bioassay, UV Spectrometry, High-performance liquid chromatography

Full Text:

INTRODUCTION

            Endophytic fungi are the probable alternative sources for the development of novel biotechnological products like antibiotics, antimycotics, immunosuppressants and anti-cancer compounds. Such microorganisms live in the plant's internal tissue which supports the plant cells growth and metabolism. Fungal endophytes have been recognized as an excellent source for new compounds of enormous value in agriculture, industry and medicine.1,2  They were the potential sources for the discovery of new and useful compounds or new platforms for the organic synthesis of such compounds for human benefit. Unlike organic synthetics, there is a clear need for endophytic sources of antimicrobials for their biological and chemical safety effects due to health and environmental problems.1,3,4

           Lovastatin is one of such secondary metabolites having huge medicinal properties and also naturally produced by certain higher fungi, such as Pleurotus ostreatus (oyster mushroom) and closely related Pleurotus sp.5 Chemical synthesis of Lovastatin strictly involves synthetic methods. It was the first statin drug which was patented5  and approved by the United States Food and Drug Administration (FDA) in 1987.6-9 The cost of production is too high for commercialization purposes and other commercially viable chemical processes only yield poor quality production and involve complicated production steps.10-13 Hence, biotechnology has opened a new avenue through simple biotechnological methods to produce high quality and cost-effective Lovastatin and it is summarized in Table 1.

        Phyllanthus reticulatus belongs to the family of Euphorbiaceae, usually a dense deciduous shrub which contains lots of medicinal properties. The different parts of this plant such as leaves, dried bark, fruits, aerial parts are to treat various diseases. In the pharmacological view, it has various activities like antidiabetic, antispasmodic, hypocholesterolemic, antimicrobial, cytotoxic, hepatoprotective anti-hyperglycemic, etc.14-19

MATERIALS AND METHODOLOGY

Collection of Plant

   Healthy leaf, stem and root of Phyllanthus reticulates (Poir)  was collected  from the Theravada regions of Sathyamangalam Taluk in a sterile ziplock bag and processed within 24 hours at the Endophytic Fungal Metabolite Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology. 20,21

Isolation and Identification of endophytic fungi

          All the plant specimens were washed thoroughly with sterile water and endophytic fungi were isolated. 22,23  The surface sterile leaves, stem and petiole were dissected under sterilized conditions into small segments of 0.5 cm x 0.5 cm using a sterile scalpel and dried in blotting paper. They were placed equidistantly (10 segments per plate per specimen) on the freshly prepared Potato Dextrose Agar plates (PDA) amended with Chloramphenicol (50 μgmL-1, Sigma Aldrich) and streptomycin sulphate (250 μgmL-1 Sigma Aldrich) and incubated at 25°C±1°C.24,25

Identification of Endophytic fungi was carried out using microscopic and morphological characteristics. Isolated pure endophytic fungal cultures were stained using lactophenol cotton blue staining method and viewed under the microscope and were shown in Figures 2,3 and 4.

Among the group of endophytic fungi isolated from the plant, best fungal isolate (Table 2) was chosen based on the highest colonization frequency and zone of inhibition in a bioassay.

Biostatistics for Species Diversity

Colonization Frequency Percentage (CF %)

The density of colonization of single endophytes species was calculated  and was equal to the number of colonized segments divided by the total number of segments observed x 100.

Endophytic Infection Rate (EIR %)

Endophytic Infection Rate was determined as the number of infected plant segments divided by the total number of plant segments screened x 100.26-30

Screening by Neurospora crassa Bioassay

This screening was based upon the use of biological responses as a detection system for biologically active substances. Neurospora crassa (MTCC 790) was sensitive to β hydroxyl acid of Lovastatin 19 and hence, Lovastatin producing isolates create a zone of inhibition by suppressing the growth of this organism. Thus, using this principle, selected 10 endophytic fungal species were screened to identify their Lovastatin producing capabilities.26,27

Batch fermentation

Highly positive endophytic fungi obtained through screening was chosen for the further fermentation process. Hence, Fully grown mycelial culture (1cm x 1cm) of Curvularia sp from the agar plate was inoculated in 100 mL of two different media M1 and M2 (Table 3) at pH 6 in triplicates under sterile condition. All the flasks were incubated in a shaker incubator at 28°C and 180 rpm for 10 days.6,14,16

Extraction and confirmation of Lovastatin production

After 8 days of fermentation, the culture broth was separated by sterilized filter cloth. The culture filtrate was adjusted from pH 6 to pH 2 and was kept in a rotary shaker with an equal volume of ethyl acetate at 100 rpm for 2hrs at room temperature. After the extraction process, the broth was centrifuged at 1500 rpm for 20 min and filtered using Whatman filter paper No.1 to separate the biomass and filtrate. The filtrate was concentrated to 20 ml using rotary evaporator .23,24,25

The presence of Lovastatin in the fermentation broth was confirmed at the maximum absorbance by UV spectrophotometry at 238nm. Further, the sample was analyzed using HPLC with a C18 column as a stationary phase and acetonitrile and water (65:35 v/v) as mobile phase.2

RESULTS

Biostatistics for Species Diversity

The colonization frequency percentage of the endophytic fungal species were shown in table 4 and the endophytic fungal infection rate percentage of Phyllanthus reticulatus was shown in figure 5.

Screening by Neurospora crassa Bioassay

The zone of inhibition formed by the endophytic fungi against Neurospora crassa is shown in table 5. Among these, the Curvularia sp shows higher zones of inhibition up to 0.6cm when compared to the other organisms. Further studies were carried out using the Curvularia sp.

Batch fermentation

Fermentation of Curvularia was carried out in both PDB and mushroom extract medium. Every 2 days, biomass was collected, filtered using pre-weighed sterilized filter cloth and dried at 60 ?C. Maximum OD of 0.826 in mushroom medium and 0.878 in PDB medium were observed at 238nm under UV spectrophotometer. The HPLC results of both the medium show that the peaks are closer enough to the standard lovastatin. It confirms the lovastatin production by Curvularia sp. The fermented biomass samples were shown in figure 6 and the HPLC results were shown in figure 7.

DISCUSSION

This study revealed that the endophytic infection rate was high in the stem segment of the selected plant and the Curvularia sp were more abundant in the leaves of the selected plant in terms of colonization frequency compared to other endophytic fungi isolated from the same plant.28,29 This may be due to the environmental suitability and interaction of Curvularia sp within the plant. This fungus has also shown a good inhibitory effect against Neurospora crassa which is one of the confirmatory tests for the production of lovastatin. Thus, this dominant fungus was chosen for the investigation of lovastatin production in a shaker flask level. The production of lovastatin by Curvularia sp in the mushroom extract and PDB medium was confirmed by UV and the HPLC. The Rf value of lovastatin  in the mushroom extract was 2.135 which is close to the hydroxyl form of standard lovastatin.30-32

CONCLUSION

          The research findings of this study conclude that Curvularia sp may be used as one of the fungal isolates for the production of lovastatin in mushroom extract medium. This novel method of using these fungi for lovastatin was reported the first time and further studies may be done to enhance productivity.

ACKNOWLEDGEMENT

We wish to express sincere gratitude to Fungal Biodiversity and BioResources Research Laboratory at Bannari Amman Institute of Technology for providing facilities for the successful completion of research work.

Authors contribution

MS - Batch fermentation, recovery of the product, report writing and edition

KPK - Biostatistics for Species Diversity and report writing

KR,  HR and GG –Screening, report writing.

Conflict of Interest: Nil

Source of Funding: Nil

References:

  1. Abd Rahim MH, Hasan H, Montoya  A, Abbas A. Lovastatin and (+)-geodin Production by Aspergillus terreus from crude Glycerol. Eng Life Sci. 2015;15(2):220-228.

  2. Akpotu MO, Eze PM, Abba CC, Nwachukwu CU, Okoye FBS, Esimone CO. Metabolites of Endophytic Fungi Isolated from Euphorbia hirta Growing in Southern Nigeria. Chem Sci Rev Lett 2017;6(21):12-19.

  3. Atli B, Yamac M, Yildiz Z. Optimization of Submerged Fermentation Conditions for   Lovastatin Production by the Culinary-Medicinal Oyster Mushroom, Pleurotusostreatus (Higher Basidiomycetes). Int J Med Mush 2013;15(5):487-495.

  4. Belwal C, Goyal PK, Balte A, Kolhe S, Chauhan K, Rawat AS, Vardhan A . Isolation, Identification, and Characterization of an Unknown Impurity in Lovastatin EP. Sci Pharma 2013;82(1):43-52.

  5. Bobek P, Ozdín L, Galbavý S.  Dose- and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats. Nutrition 1998;14 (3):282–286.

  6. Lopez C, Sanchez Perez JA, Fernández Sevilla JM, Acien Fernandez FG, Molina Grima E & Chisti  Y.Production of Lovastatin by Aspergillus terreus: effects of the C: N ratio and the Principal Nutrients on Growth and Metabolite Production. Enzy Microb Tech 2003;33(2-3):270-277.

  7. Chang YN, Huang JC, Lee CC, Shih IL, Tzeng YM. Use of response surface methodology to optimized medium for the production of lovastatin by Monascusruber. J Enzy Microbio 2002;30(7): 889-894.

  8. Chi SM, Wang Y, Zhaw Y, Pu JX, Du, X, Liu JP, et al. A New cyclopentanone derivative from Euphorbia hirta. Chem Nat Compd 2012;48(4):577-579.

  9. Dobranic JK, Johnson IA, Alikhan QR. Isolation of Endophytic Fungi from Eastern Larch (Larix Laricina) Leaves from New Brunswick, Canada. Can J Microbiol 1995; 41(2):194-198.

  10.  Eyob CC, Raju KC. Endophytic Mycoflora and Their Bioactive Compounds from  

 Azadirachta indica: A Comprehensive Review. J Mycol 2018;4(2):1-12.

  1.  Fischer J, Robin G. Analogue-based Drug Discovery. John Wiley & Sons. 2006;4:472.

  2.  Fisher PJ, Petrini O. A comparative study of fungal endophytes in xylem and  

             bark of Alnus Species in England and Switzerland. Mycol Res 1990;94(3):313-349.

  1.  Gangadevi V, Muthumary J. Taxol an anticancer drug produced by an endophytic fungus Bartaliniarobillardoides Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb. World J Microbio Biotech 2008;24(5):717-724.

  2.  Hajjaj H, Niederberger P, Duboc P. Lovastatin Biosynthesis by Aspergillus terreus in a Chemically Defined Medium. Appl Envt Microbio 2001;67(6):2596-2602.

  3.  Hirama M, Iwashita M. Total synthesis of (+)-monacolin K (mevinolin). Tetrahedron Lett 1983; 24(17):1811-1812.

  4. Karthika C, Sharmila G, Muthukumaran C, Krishnan M. Utilization of Whey Powder as an Alternate Carbon Source for Production of Hypocholesterolemic Drug by Aspergillus terreus MTCC 1281. J Food Sci Biotechnol 2013;22(5):1-7.

  5. Kumar DSS, Hyde KD. Biodiversity and Tissue-Recurrence of Endophytic Fungi in Tripterygium wilfordii. Fungal Divers 2004;17(1):69-90.

  6. Kumar DSS, Lau CS, Wan JMF, Yang D, Hyde KD. Immunomodulatory compounds from Pestalotiopsis leucothes (HKUCC 10197), an Endophytic Fungus of Tripterygium wilfordii. Life Sci 2005;78(1):147-156.

  7. Kumar MS, Jana SK, Senthil V, Shashanka V, Kumar SV, Sadhukhan AK. Repeated Fed-Batch Process for Improving Lovastatin Production. Proc Biochem 2000; 36(1):363-368.

  8. Kumar MS, Kumar PM, Sarnaik HM, Sadhukhan AK. A rapid technique for screening of lovastatin-producing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay. J Microbiol Meth 2000;40(1):99-104.

  9. Lai LS, Hung CS, Lo CC. Effects of lactose and glucose on the production of itaconic acid and lovastatin by Aspergillus terreus ATCC 20542. J Biosci Bioeng 2007;104(1):9-13.

  10. Lai, LST, Pan CC, Tzeng  BK. The influence of medium design on lovastatin production and pellet formation with a high producing mutant of Aspergillus terreus in submerged cultures.Proc Biochem 2003;38(9):1317-1326.

  11. Lee CL, Wang JJ,  Pan TM.Synchronous Analysis Method for Detection of Citrinin and the Lactone and Acid forms of Monacolin K in Red Mold Rice. J Biochem 2006;89(89):669-677.

  12. Luthra U, Singh N, Tripathi A, Vora S, Bhosle V. Media Optimization for Lovastatin    Production by Statistical Approach using Aspergillus terreus by Submerged Fermentation. Int J  Res Med Sci 2015;3(2): 4520-4528.

  13. Parija SC, Shivaprakash MR, Jayakeerthi SR.Evaluation of Lacto-phenol cotton blue (LPCB) for detection of Cryptosporidium, Cyclospora and Isospora in the wet mount preparation of stool. Acta Trop 2003;85(3):349-354.

  14. Senthamarai M, Kannan KP.Optimization of lovastatin production by Fusarium nectrioides (MH173849) using response surface methodology and fuzzy logic system. J Environ Biol 2019;40(5):1036-1044.

  15. Samiee SM, Moazami N, Haghighi S, Mohseni FA, Mirdamadi S, Bakhtiari MR. Screening of Lovastatin Production by Filamentous Fungi. Iran Biomed J 2003;7(1):29-33.

  16. Sharma S, Kumar S. Phyllanthus reticulatus Poir. – An important medicinal plant: A review of its Phytochemistry, Traditional uses and Pharmacological properties. Int J Pharm Sci Res 2013:4(7);2528-2534.

  17. Su YC, Wang JJ, Lin TT, Pan TM. Production of the secondary metabolites γ-aminobutyric acid and monacolin K by Monascus. J. Ind Microbiol Biotech 2003;30(1):41-46.

  18. Suryanarayanan TS, Kumaresa V and  Johnson JA. Foliar fungal endophytes from two species of the mangrove Rhizophora. Can J Microbiol 1998;44(10):1003-1006.

  19. Vagelos PR, Galambol L. Medicine, Science and Merck. Cambridge University Press, Cambridge, United Kingdom.2004;211-301.

  20. Wang  F W., Jiao R H., Cheng A B, Tan S H, Song Y C. Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp. World J Microbiol Biotech 2007;23:79–83.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A study by Dorothy Ebere Adimora et al. entitled \"Remediation for Effects of Domestic Violence on Psychological well-being, Depression and Suicide among Women During COVID-19 Pandemic: A Cross-cultural Study of Nigeria and Spain\" is awarded Best Article of Vol 14 issue 23
A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22
A study by Saurabh Suvidha entitled \"A Case of Mucoid Degeneration of Uterine Fibroid with Hydrosalphinx and Ovarian Cyst\" is awarded Best article of Vol 14 issue 21
A study by Alice Alice entitled \"Strengthening of Human Milk Banking across South Asian Countries: A Next Step Forward\" is awarded Best article of Vol 14 issue 20
A study by Sathyanarayanan AR et al. entitled \"The on-task Attention of Individuals with Autism Spectrum Disorder-An Eye Tracker Study Using Auticare\" is awarded Best article of Vol 14 issue 19
A study by Gupta P. et al. entitled \"A Short Review on \"A Novel Approach in Fast Dissolving Film & their Evaluation Studies\" is awarded Best Article of Vol 14 issue 18.
A study by Shafaque M. et al. entitled \"A Case-Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and CoAmoxicillin in Patients with Chronic Suppurative Otitis Media\" is awarded Best Article of Vol 14 issue 17
A study by Ali Nawaz et al. entitled \"A Comparative Study of Tubeless versus Standard Percutaneous Nephrolithotomy (PCNL) \? A Randomized Controlled Study\" is awarded Best Article for Vol 14 issue 16.
A study by Singh R. et al. entitled \"A Prospective Study to Find the Association of Astigmatism in Patients of Vernal Keratoconjunctivitis (VKC) in a Tertiary Health Care Centre in India (Vindhya Region MP)\" is awarded Best Article for Vol 14 issue 15
A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: To achieve a high standard of publication, we adopt Good Publishing Practices (updated in 2022) which are inspired by guidelines provided by Committee on Publication Ethics (COPE), Open Access Scholarly Publishers Association (OASPA) and International Committee of Medical Journal Editors (ICMJE)

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



ABOUT US

International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2024 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions