International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - vol 08 issue 08, April, 2016

Pages: 04-07

Date of Publication: 20-Apr-2016


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DETERMINATION OF BACTERIOCIDAL ACTIVITY OF BACTERIOCIN PRODUCED BY A CLINICAL ISOLATE OF E.COLI AGAINST HUMAN PATHOGENS

Author: Joseph Pushpa Innocent D., Margaret Theresa J., Premkumar D.

Category: Healthcare

Abstract:Aim: The aim of this study is to estimate the bactericidal activity of bacteriocin against the common human pathogens. A total of 62 specimens were collected from patients attending the department of surgery, Karpaga Vinayaga Institute of Medical sciences and Research Centre, during a period of six months. Methodology: Specimens collected were superficial pus swab from the wound, deep tissue biopsy and scraping from the base of the ulcers. Samples were cultured on Blood agar (BA) and MacConkey agar (MA). The plates were incubated at 37C over night. The aerobic organisms were identified by standard microbiological procedures as described in Bailey and scott's Daignostic Microbiology. (Finegold et al.1986).(1) The E coli which was isolated and identified was used in the preparation of crude bacteriocin.( 2) It was purified by Ammonium sulphate precipitation(3)and concentrated by centrifugation. Desalting of protein was done by dialysis method.(4) The protein concentrations of these purified bacteriocin was determined by Lowry's method.(5) Bacteriocin prepared from E coli was subjected for its bactericidal activity against some human pathogens by minimum inhibitory concentration method. Optical density (OD) of the culture was measured at 660 nm using spectrophotometer. Bactericidal activity of bacteriocin against human pathogens was estimated. Results: The results were analyzed, most of the pathogens need a very low concentration of the bacteriocin for the minimum inhibitory concentration (MIC).We observed that there is a slight variation in MIC among the different bacterial pathogens. Conclusion: In General when the concentration of the bacteriocin increased then the OD values of the culture tubes were decreased. In conclusion Bacteriocin not only have the bacteriocidal activity but also dose dependent in different bacteria.

Keywords: Bacteriocin, Bactericidal activity, Microbial virulence

Full Text:

INTRODUCTION Pathogenic microbes produce disease in man is due to certain enzymes and toxic substances released by bacteria called virulence factors. Some chemicals released by the bacteria may cause self destruction are known as autolytic enzymes. Similarly some other biologically active compounds, produced by the bacteria may active against strains of other species or closely related species. This type of bactericidal agents produced by Gram negative bacteria are called bacteriocins.(6) Bacteriocins are ribosomally synthesized antimicrobial peptides.(7) Classification of bacteriocins based on molecular size, producer organisms, chemical structure and the mode of action. Various names are given to these agents such as colicin, pyocin and or marcescins etc. Bacteriocin affect the essential function of livings cells such as transcription, replication and cell wall biosynthesis.(8) They also act by forming membrane channels or pores that destroy the energy potential of sensitive cells.(9) However bacteriocin producing strains are resistant to their own product. It has been evaluated for intended use as mouth wash since it kills the bacteria which cause dental plaque. Hence it is safe to use as a natural alternative to antibiotics and chemical germicides. Keeping this in mind, it was planned to study the bactericidal activity of bacteriocin on some of the human pathogens. Informed consent was obtained from those who underwent the test procedure in this study. Ethics committee of Dr. MGR University approved this study.

MATERIALS AND METHODS A total of 62 specimens were collected from gulutial, thigh, knee, leg, foot, breast and umbilical areas which included the wound swabs, pus, tissue biopsy and scraping from ulcers. Samples were collected aseptically from patients attended the surgery department of Karpaga Vinayaga Institute of Medical science and Research Centre, Chinnakolambakkam. Samples collected were inoculated on to culture media and the organisms grown after incubation were identified by standard microbiological methods. Among the organisms isolated, the E. coli was the most commonly observed (10) The clinically isolated E.coli was used in the preparation of crude bacteriocin, because it is the common source of bacteriocin.(11) E. coli isolates had been grown on nutrient broth with 5% inoculums by batch fermentation at a pH of 6.2. The broth culture was incubated at 37c for 12 hours. For the separation of bacteriocin the culture was centrifuged at 8000 rpm for 20 minutes. Cell free supernatant was subjected to solid ammonium sulphate at pH 4 with gradual increase of 20 to 90% saturation and held for 3hours at 4c. The mixtures were centrifuged at 12000 rpm for 20 minutes. The precipitate was recovered and it was then suspended in 5ml of 50m.mol of sodium phosphate buffer at pH 7 followed by desalting of the protein by dialysis. Protein concentration was determined by lowry’s method and compared with the standard bovine serum albumin. Estimation of bactriocin activity The purified bacteriocin compound from E.coli was subjected for its bacteriocidal activity against commom human pathogens. Organisms used for the assay were Klebsiella spp, Salmonella typhi, staphylococcus aureus and corynebacterium. Estimation of MIC for the above culture of organisms, 0.5 ml of each log phase cultures were transferred in to test tubes contain nutrient broth of appropriate concentration of bacteriocin. 100µl/mL to 500µl/ml were prepared and added to 8ml of broth culture and were inoculated at 37c for 24hours. Minimum inhibitory concentration was estimated by measuring the optical density of the culture tubes at 660nm using spectrophotometer.

RESULTS Antibacterial sensitivity of bacteriocin against human pathogens were estimated and the results were tabulated .Table: 1 showed the turbidity measurements of klebsiella spp 

DISSCUSSION Bacteriocins are the subject of increasing attention as a major replacement for the conventional antibiotics and as natural food preservatives.(12) There are multiple bacteriocins present in the population of E.coli (13) and (14). Colicin production and the effect of growth inhibition was tested by solid methods.(15) In the present study turbidometry was used to assay the growth inhibition. In medicine the bacteriocins are important as they are produced by some non pathogenic bacteria act as commmonsal and loss of these bacteria following the use of antibiotics may cause the invasion of opportunistic pathogens. Hence it is decided to demonstrate the effect of bacteriocin on human pathogens. Further most of the mechanisms of bacteriocin in killing the bacteria are similar to that of antibiotics.(16)(17) Therefore it may replace the use of the antibiotics in the treatment. Hence this study was conducted as a step to determine the effect of bacteriocin on human pathogens.

CONCLUSION Though MIC studies of bacteriocin on human pathogens may not showed any consolidated report, but the results were encouraging. Present study results revealed that some strains of bacteria were sensitive to bacteriocin and are showed a response to its concentration. Each of the pathogen responded differently. Further studies are essential with still highly purified form of bacteriocin and the pathogens to be studied with wide range of bacteriocin concentration.

ACKNOWLEDGEMENT Authors acknowledge the immense help received from the scholars whose articles are cited and included in references of this manuscript. The authors are also grateful to authors/ editors/ publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed.

References:

1. Finegold, S.M., Baron E.J. 1986. In. Bailey and Scott’s Diagnostic Microbiology. VII. Edn. Masby Company. St Louis. P. 106-125.

2. Yildirim. Z and Johnson M.G; ( 1998) Detection and characterization of a bacteriocin produced by Lactococcus lactis subsp. cremoris R isolated from radish,Latters in Applied Microbiology,26, 297-304.

3. Klaenhammer,T.R;(1998) Biochim, 70, 337-349.

4. Rajaram. G,Manivasagan.P,Thilagavathy .B, Saravanakumar .A.(2010) Purification and charactrization of a bacteriocin producied by Lactobacillus lactis isolated from marine environment. Advan J. food scie, and technology 2 (2) 138 – 144.

5. Lowry, O.H, Rosebrough .N.J, Farr A.L and Randall.R.J. (1951) Protein measurement with the folin –phenol reagent. J. Biol. chem.,193:265-275.

6. Gillis, R.R.,(1957) Colicin production as on epidemiological marker of Shigella sonnei,J.Hyg , 62, 1-9

7. Klienkauf ,H.V on Dohren, H.,(1990) Non ribosomal biosynthesis of peptide antibiotic, Eur ,J. bio chem. 192, 1-15.

8. Lakey,J.H. Gonzalezmanas,J.M.et al ., (1992) The membrane insertion of colicins, FEDS Lett, 307, 26-29.

9. Riley,M.A.,(1993) Positive secretion for colicin diversity in bacteria, Mol; Boil vol

10, 1048-1058. 10. Saleem M;Joseph Pushpa Innocent D.(2014) Comparison of microbial flora by deep tissue biopsy and superficial swab culture of specimen collected from various anatomical sites in early wound infections Int J Cur Res Rev, Vol 6.Issue 18, 24-28.

11. Shih-Chun yang, Chih- Hung Lin, Calvin T. Sung, and Jia-You Fang (20140) Antibacterial activities of bacteriocins: application in foods and pharmaceuticals Front Microbiol vol 5;241

12. Gillor O, Kirkup BC, Riley MA (2004) Colicins and Microcins the next generation antimicrobials. In Laskin BennettJW, M. Gadd G.Adv Appl. Microbiol Academic Press P 129-146

13. Margaret A. Riley, John E, Wertz (2002) Bacteriocin diversity: ecological and evolutionary prespecitves Biochimic 84-357- 364.

14. Maria Papagianni, Nicholaos Avramidis, George Filioussis, Despina Dasiou and loannis Ambrosiadis (2006) Determination of bacteriocin activity with bioassays carried out on solid and liquid substractes assessing the factor indicator Microorganism Microbial cell Factories 5: 30

15. David Smajs, Lenka Mioenkova, Jan Smarda, MartinVrba a(2010) Alena Sevcikova Zuzana valisova and Commensal Escherichia coli: colicin E1is a potential virulence factor BMC. Microbiology 10: 288.

16. Renee’ Pieterse; Svetoslav D.;Todorov (2010) Bacteriocins-exploring alternatives to antibiotics in mastitis treatment Braz J. Microbiol. Vol 41 no 3 Sao Paulo Oct 2010.

17. Cotter, P.D; Hill, C; Ross, R.P. (2005) Bacteriocins: Developing innate immunity for food. Nature Rev. Microbiology; 3;777- 788.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

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Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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