International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - Vol 09 Issue 22, November, 2017

Pages: 01-04

Date of Publication: 30-Nov--0001


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Variation of Andrographolide Content in Andrographis paniculata from Different Sites of Balaghat Region of Jabalpur (M.P.)

Author: Anil Kushram, S. K. Masih, Sajad Ahmad Mir

Category: General Sciences

Abstract:Aim: The main objective of this investigation was to show the variation of Andrographolide content in Andrographis paniculata from thirty seven different sites of Balaghat (Jabalpur MP) region.
Methodology: In order to study the variation in andrographolide content, plant material was collected from different sites and were analyzed through quick and accurate HPLC chromatographic method using C-18 column (4.6 x 250 mm, 5\? particle size).
Result and Discussion: The average andrographolide content varied from 0.01 to 1.2%. Highest percent of andrographolide in Andrographis paniculata were estimated from site Kera of south Balaghat 1.2%. Lowest content of andrographolide in Andrographis paniculata were estimated from the site of Salhen north Balaghat region that is 0.02%.
Conclusion: The content of this active ingredient in this plant varies with in the plant parts and with the geographical distribution.

Keywords: Andrographis paniculata, Andrographolide, Balaghat region, HPLC instrumentation

DOI: 10.7324/IJCRR.2017.9221

Full Text:

INTRODUCTION

 Andrographis paniculata is an important medicinal plant of family acanthaceae. It is also called king of bitters. It is an annual herb widely used in different parts of Asia. Used mainly for treating fever, liver disease, common cold, chickengunia fever, snake bite etc. It is to be used extensively for medicinal purpose in India, Thailand, China and Mauritius (Niranjan, et. al., 2010). The leaves and aerial parts of the plant are used in Indian traditional medicine for the treatment of fever, malaria, some throat infection (Shanker, et. al., 2012), hepatoprotective, antioxidant (Lin, et. al., 2009). Whole plant has variety of therapeutic uses. Phytochemical data revealed that this plant can be used for the treatment of common cold pharyngotonsillitis and diarrhea (Mishra et al 1992 and Najib et al 1999). Antioxidant properties of Andrographis paniculata were recorded by (Trivedi and Raval, 2001). This plant is used for blood purifier, liver diseases, dermatological diseases (Prathanturarug et al., 2007). Aqueous extract of this plant are having antimicrobial activity due to presence of arabinogalactan proteins and andrographolide (Singh et al., 2003). Leaf part was found to content highest amount of andrographolide and the seeds were estimated to content lowest amount of andrographolide (Sharma et al., 1991). Andrographolide is bitter crystalline compound, colorless having antithrombotic, hyphoglycemic and antipyretic properties. A recent study conducted by USA University that this plant is having anti HIV activity (Calabrese, 2000). This plant ranks seventeenth position among their thirty two prioritized medicinal plants and annual growth of this plant is 3.1 percent (Anonymous, 2007).

MATERIALS AND METHODS

Plant material: Andrographis paniculata was collected from different sites of Balaghat region. Areal plant parts were collected for comparative study of andrographolide content. Leaves/ plant material at different sites were collected and dried in shade for 15 days followed by grinding to form powder of it. The fine powdered samples were used in methanol solvent to estimate andrographolide content by HPLC method.

HPLC Instrument: HPLC instrument that was used for the estimation of andrographolide were of the following features, HPLC- grade waters, Pump - 515 Isocratic pump, Injector - Rheodyne injector with a 20-microlitre loop, Detector - UV Vis detector, Software - Data ace software, Column - Thermo C-18 column (4.6 x 250mm, 5μ particle size), sample size (20µl). Isocratic elution was carried out with methanol at a flow rate (1ml/min). The detection was performed with wavelength (230 nm) and column temperature was ambient (300C). Class VP software was used for integration and calibration. Evaluation was via peak areas with linear regression.

Preparation of herbal extract: Fresh aerial part of the plant sample collected from 37 different sites of Balaghat region of Jabalpur (M.P). Reflux 1 gm dried powder along with 50 ml of methanol was kept in soxhlet for one hour. After one hour the refluxing load was subjected to Rota-vapor at 60 RPM and heated at 600C. Filter & subject the marc for another two cycles of refluxes (1 hrs. each) with methanol (50 ml) combine with the filtrate. Evaporate under vacuum to dryness Dissolve the residue 10 mg in methanol (10ml). Filter, Inject the solution in HPLC with the help of 20 µl fixed loop injector and percent content of andrographolide were estimated by counting the area of andrographolide peak in HPLC chromatogram in all sample.

RESULTS

Table 1: Variations of Andrographolide content from Andrographis paniculata

References:

  1. Anonymous, (2007). National Medicinal Plant Board, Asia-Pacific Forestry Sector Outlook Study II. Country report, Ministry of Environment & Forests, New Delhi.
  2. Calabrese, C. et al., (2000). A phase I trial of andrographolide in HIV positive patients and normal volunteers. Phytotherapy Research, 14: 333-338.
  3.  Lin, F.L., Wu, S.J., Lee, S.C. and Ng, L.T., (2009). Antioxidant, antioedema and analgesic activities of Andrographis paniculata extracts and their active constituent andrographolide.  Phytotherapy Research, 23 (7): 958 - 964.
  4. Mishra, P., N.L. Pal, P.Y. Guru, J.C. Katiyar, Srivastava, V. and Tandon, J.C., (1992). Antimalarial activity of Andrographis paniculta (Kalmegh) against Plasmodium berghi NK 65 in Mastomys natalensis. International Journal Pharmacology, 30: 263-274.
  5. Najib, Nik A., N. Rahman, T. Furuta, S. Kojima, Takane, K. and Ali Mohd, M., (1999). Antimalarial activity of extracts of Malaysian medicinal plants. Journal of Ethnopharmacology, 64(3): 249-54.
  6. Niranjan, A., Tewari, S.K. and Lehri, A., (2010). Biological activities of Kalmegh (Andrographis paniculata Nees.) and its active principles-a review. Indian Journal Of Natural Product and Resources, 1:125- 135.
  7. Prathanturarug, S., N. Soonthornchareonnon, Chuakul, W. and Saralamp, P., (2007). Variation in growth and diterpene lactones among field-cultivated Andrographis paniculata. Journal Natural Medicine, 61: 159-163.
  8.  Shankar, R.S., Deb and Sharma, B.K., (2012). Antimalarial plants of northeast India: An overview. Journal of Ayurveda Integrative Medicine, 3(1): 10-16.
  9. Sharma, A.K., Singh, B.B. and Singh, S.P., (1991). Relationship among net assimilation rate LAI and yield of soybean of genotypes. Photosynthetica 16: 115-122.
  10. Singh, P.K., Roy S. and Dey, S., (2003). Antimicrobial activity of Andrographis paniculata. Fitoterapia 74 (7-8): 692-694.
  11. Trivedi, N.P. and Rawal, U., (2001). Hepatoprotective and antioxidant property of Andrographis paniculata (Nees) in BHC induced liver damage in mice. Indian Journal of Experimental Biology, 39(1): 41-46.

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Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

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Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by situs slot and Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh et al. situs slot "Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma situs slot entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by situs slot et al entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09
Late to bed everyday? You may die early, get depression situs slot
Egg a day tied to lower risk of heart disease situs slot
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List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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