IJCRR

IJCRR - 2(2), February, 2010

Pages: 33-42

Print Article   Download XML  Download PDF

QUANTITATIVE ANALYSIS OF FLUTAMIDE IN BULK DRUG AND ITS PHARMACEUTICAL DOSAGE
FORM BY USING HPLC

Author: S.Sudharshini1, Kumar Choudhury, Bhupendra shrestha

Category:

Abstract:A simple, rapid and reproducible high performance reverse phase liquid chromatographic method has been developed for quantitative estimation of Flutamide in tablets using a C-8 column and UV detection at 294nm. The isocratic elution was used to quantify the analyte. The samples were chromatographed on C-8 column and the mobile phase was Acetonitrile: Water (50:50, v/v) was pumped at 1 mL/min. The method was linear between 10- 125µg mL-1 , statistically validated for its linearity, precision and accuracy. The intra-and - inter day variation was found to be less than 1% showing high precision of the assay method. It was found that the excipients in the commercial tablets did not interfere with the method.

Keywords: Flutamide, High performance liquid chromatography (HPLC), Acetonitrile

Full Text:

Key words:

Flutamide, High performance liquid chromatography (HPLC), Acetonitrile

Introduction

Flutamide1-3 is an acetanilide, nonsteroidal compound with orally active antiandrogenic properties. It exerts its antiandrogenic action by inhibiting androgen uptake and/ or nuclear binding of androgen in target tissues. It is used, usually with gonadorelin analogues; in the palliative treatment of prostatic carcinoma. It is freely soluble in Acetone, Ethyl acetate, Methyl alcohol.