International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - Vol 03 Issue 03, March, 2011

Pages: 52-58

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DEVELOPMENT OF FINGERPRINTS FOR SINGLE COMPONENT ANALYSIS OF AN AYURVEDIC
FORMULATION (PIPPLI CHURNA) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Author: Gupta Vishvnath, Jain U. K.

Category: Technology

Abstract:Pippli Churna (PC) is a most popular Ayurvedic formulations among the Ayurvedic medicines,
Piperine is one of the major constituent of Pippli Churna. The process of development of HPLC
fingerprints for Pippli Churna extract is introduced in detail. The three laboratory batches and
three marketed batches were taken in this study to estimate the % of piperine in this indigenous
formulation. The selection of a suitable chromatographic system, the screening for important
parameters, and gradient optimization to method validation, and an integrated and universal
HPLC fingerprint approach was performed. This improves the separation quality of the
fingerprint. The detection wavelength of piperine was 343 nm. Thethree laboratory batches and
three marketed batches were taken in this study to estimate the % of piperine in this indigenous
formulation. The results of the method validation, based on the relative standard deviation of
relative retention times and relative peak areas, were acceptable. Calibration curves showed good
linearregression (R2 > 0.999) within test range. The LODs and the LOQs for the piperine were
0.063 mg/ml and 0.071 mg/ml. This strategy is used for the estimation of piperine in Pippli
Churna(PC) formulation and identifies and assessedits quality. Results of statistical analysis show
present HPLC method for determination of Piperine is simple, precise, accurate and suitable for
routine analysis of Piperine in PC. The developed fingerprints can be used as a standard and
Piperine can be used as a possible marker compound for fingerprinting of PC.

Keywords: Fingerprints, Pippli churna (PC), Piper species, Piperine marker, HPLC.

Full Text:

INRODUCTION

India has a vast heritage of traditional system of medicine (Ayurveda, Siddha and Unani), due to the lack of precise quality control measures and finger printing methods, the benefits of these systems remains largely underutilized 1. Keeping this thing in mind, from the last two decades, efforts have been made in developing quality control parameters for Ayurvedic formulations by means of chromatographic finger printing methods2,12. World Health Organization (WHO) has emphasized the need to ensure the quality of medicinal plant products by using modern controlled technique and applying suitable standards8 . For standardization of natural product drugs,

single chemical entities, “marker compounds,” may be used as potency standards in high performance liquid chromatography (HPLC) analysis. These marker compounds may be used to help identify herbal materials, set specifications for raw materials, HPLC analysis for marker compounds may provide additional information in the form of chromatographic fingerprints. The present study is undertaken to develop certain fingerprints for an Ayurvedic formulation. Pippli churna used for cough and cold, spleen disorders, fever, diabetes, piles, tuberculosis, abdominal disease ,thrust warms, leprosy, pain ( colicspasmodic) and digestive impairments. Pippli churna possesses bioavailability enhancing properties.,ie. antitubercular drugs and antilaprotic drug. The essential oils of pipali churna have antibacterial and antifungal activities. Ayurvedic formulary of India has given the specification for the composition of PC, it should contain piper species as a major ingredient apart from different herbs3,4 .

AIMS AND OBJECTIVES

Pharmacopoeial standards for Ayurvedic formulations published by the Central Council for Research in Ayurveda and Siddha gives certain physical parameters as standards for churna, these standards are not based on modern analytical methods5 . It is therefore essential to develop definite and accurate analytical tools to as certain consistency and quality of Ayurvedic preparation from batch to batch in pharmaceuticals which may results in acceptability world wide. In present study we tried to develop a method that serves as fingerprinting method for Pippli churna(PC).

EXPERIMENTAL

All the solvents for HPLC analysis were HPLC grade and purchased from E. Merck and S. D. Fine Chemicals, Mumbai. All solvents used for extraction were primarily distilled before use. SHIMADZU – LC10AT HPLC was used for piperine analysis. All the results are obtained by repetition of the each experiment six times (n= 6).

Procurement of drug

Crude drugs were procured from local market and identified by macroscopic10,11 and microscopic characters6,7,8,9 .

Preparation of formulations

1. Three batches were prepared in laboratory (named as PC-I,PC-II and PC-III) according to strict methods of „Ayurvedic formulary of India? and Sarangadhara-samhita. 2. Commercially available brands PC-A, PC-B, and PC-C, of Pippli churna were procured from local market.

Sample preparation for estimation of Piperine content 1.5 gm Pippli churna was refluxed for 1 hour with 100 ml of methanol. The volume were reduced under pressure and filtered by 0.2 μm membrane filters. The filtrate was diluted up to 100 ml with methanol. To the 20 ml of resulting solution, 2 ml of 0.5- mg/ml solution of p-dimethyl amino benzaldehyde (internal standard) was added, and made the final volume 25 ml with methanol .7,12 [Fig. 1].

Preparation of standard solution Piperine was purchased from Lancaster, England. Standard solution was prepared by the addition of 2 ml of solution a (1mg/ml) of Piperine and 2 ml of internal standard solution (0.5 mg/ml of p-dimethylamino benzaldehyde) in a 25 ml volumetric flask made the final volume to 25 ml with methanol.

HPLC studies7 Estimation of Piperine was carried from different batches (three marketed and laboratory batch) of PC with following conditions:

Column : C 18 (25cm X 4.6 mm i.d.) 10 μ,

Mobile phase: methanol: water (69:31),

Detection: at 343 nm (reference wavelength: 343 nm),

Injection volume: 20 μl and

Flow rate: 1.5ml/min

Calibration The Piperine content of PC was determined using a calibration curve established with seven dilutions, at concentrations ranging from 0.5-20 μg/ml. Each concentration was measured in triplicate. The corresponding peak areas were plotted against the concentration of the Piperine injected. Peak identification was achieved by comparison of both the retention time and UV absorption spectrum with those obtained for standards.

 

Validation parameters

Selectivity and peak purity Selectivity was checked by using prepared solutions of PC and available standards optimizing separation and detection. The purity of the peaks was checked by multivariate analysis. The three spectra corresponding to up slope, apex and down slope of each peak were computer normalized and super imposed. Peaks were considered pure when there was a coincidence between the three spectra (match factor was =98%).(Table-I)

Linearity, limits of detection and quantification The linearity of the detector response for the prepared standards was assessed by means of linear regression regarding the amounts of each standard, measured in μg, and the area of the corresponding peak on the chromatogram. Linearity was also confirmed for PC prepared sample solutions. After chromatographic separation, the peak areas obtained were plotted against concentrations by linear regression. Limits of detection and quantification were determined by calculation of the signal-tonoise ratio. Signal-to-noise ratios 3:1 and 10:1 were used for estimating the detection limit and quantification limit, respectively, of the method.

 

Precision

The repeatability of the injection integration was determined for both standard piperine and the content of piperine in Pippli churna. A standard solution containing reference compounds and prepared sample solutions was injected. Pippli churna samples were also prepared 2 times to evaluate the repeatability of the process. The mean amount and R.S.D. values were calculated. The precision was calculated at two different concentrations high and low tested in the concentration range. For standardization the sample was injected at eight different concentrations and linearity was noted [Table-1].

Accuracy

The accuracy of the method was determined by analyzing the percentage of recovery of the piperine in the Pippli churna. The samples were spiked with two different amounts (100, 150 μg) of standard compounds before sample preparation. The spiked samples were extracted by triplicate and analyzed under the previously established optimal conditions. The obtained average contents of the target compounds were used as the “real values” to calculate the spike recoveries [Table-1].

Robustness For the determination of the method?s robustness a number of chromatographic parameters, such as column package and size, mobile phase composition and gradient ratio, flow rate and detection wavelength, were varied to determine their influence on the quantitative analysis. Interday and intraday variability was studied for the sample, by injecting the same concentration of the sample on three different days and the standard error mean was calculated.

RESULTS AND DISCUSSION In the present study, spectral and chromatographic studies were performed. Results of the The RP-HPLC analyses of PC were performed, samples were injected at seven different concentrations and the linearity was observed with in the concentration range of 0.5-20 μg/ml [Fig. 2].Both Piperine was well separated at retention time 8.020 respectively. The concentration of Piperine present in raw material was found to be 1.41±0.62 w/w in Piper longum fruits (pippli). The content of Piperine in laboratory formulations (PC-I, II, III) were found to be 0.16±0.002 ,0.19±0.006 ,and 0.18±0.004 respectively and in different marketed formulations of PC were, for PC-A (0.15 ±0.002 %), PC-B (0.16±0.004%), PC-C (0.17±0.008 %) w/w respectively [Table-2, Fig-1]. The HPLC method was validated by defining the linearity, peak purity, limit of quantification and detection, precision, accuracy, specificity and robustness. For the qualitative purposes, the method was evaluated by taking into account the precision in the retention time, peak purity, and selectivity of piperine elutes. A high repeatability in the retention time was obtained with (R.S.D.) value lower than 1.5% for both standard and samples even at higher concentration. (Table-3).The peak purity was studied in the major peaks. Linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision were evaluated for quantitative purposes [Table-1].Thus LOD and LOQ found to be 0.063, 0.071mg/ml respectively which suggest full capacity for quantification of piperine content in different laboratory and marketed batches of PC. R2 value for the regression equation of the Piperine was higher than 0.9988 thus confirm the linearity of the method. The recovery study was performed at two levels by adding known amount (100, 150 μg/ml) of piperine with reanalyzed sample of PC found to be close to 99.48(mean)% and a higher repeatability indicate a satisfactory accuracy in the proposed methods [Table- 3].Finally the robustness of the method was also assessed. Minor modification of the initial mobile phase gradient (from 25 to 30% solvent instead of 31%) had no effect on the peak resolution of the compound. Therefore, this HPLC method for fingerprinting of PC can be regarded as selective, accurate, precise, and robust. The method is very adaptable because of the precision and repeatability for the traditional Ayurvedic formulation like PC and suitable for routine analysis of Piperine in PC. There was not much variation in the interday and intraday injections performed with the R.S.D. value was found to be 0.080%with the mean standard error 0.03% respectively. Piperine estimation can be utilized as a possible analytical marker for fingerprinting of Pippli churna.

CONCLUSION

The developed high performance liquid chromatographic method for estimation of Piperine from Pippli churna could be used as a valuable analytical tool in the routine analysis, to check the batch to batch variation. Estimation of Piperine can be used as one of the appropriate analytical markers for the finger printing.

ACKNOWLEDGEMENT

The authors are grateful to Principal, BITSPharmacy college , Bhopal for their unforgetable support.

References:

1. Mukherjee PK. Quality Control of Herbal Drug. Ist edition, New Delhi: Eastern Publishers (Business Horizontal Ltd.) 2002;03-30,184-219.

2. Jain V, Saraf Swarnlata, Saraf S. Standardization of Triphala Churna: Spectrophotometric approach. Asian J of Chem 2007;19(2):1406-10.

3. Kokate CK, Purohit AP, Khandelwal KR. Pharmacognosy, 29th edition, New Delhi: Nirali Prakashan 2002;550-9.

4. The Ayurvedic Formulary of India, PartI, II nd edition, New Delhi: Govt. of India, Ministry of Health and Family 58 International Journal of Current Research and Review www.ijcrr.com Vol. 03 issue 03 Mar 2011 Planning, Department of Health 2003,103-19

. 5. Pharmacoepical standards for Ayurvedic Formulations. Central Council for Research in Ayurvedic and Siddha, New Delhi: Ministry of Health and Family Welfare 1987;112-23.

6. Quality Standards of Indian Medicinal Plants. Volume I and II, New Delhi: Indian Council of Medicinal Research 2003;95,168

7. Indian Herbal Pharmacopoeia, Volume II, Mumbai: Indian drug Manufacturing Association and Regional Research Laboratory Jammu 1999;90-3,126-62.

8. World Health Organization, Quality Control Methods For Medicinal Plants Materials, Geneva : 1998,1-15.

9. Khandelwal KR. Practical Pharmacognosy Techniques and Experiments. 9th edition, New Delhi, Nirali  Prakashan 2002;152.

10. Wagner H. Plant drug analysis, a thin layer chromatography atlas, II nd edition, Springer 2002 298. 10. Wagner H, plant drug analysis, a thin layer chromatography atlas, IInd edition, springer 2002, 298.

11. Kokate C.K. Practical Pharmacognosy, IVth edition , New Delhi : Vallabh Prakashan 1996; 143.

12. Jain V, Saraf Swarnlata, Saraf S. Spectrophotometric Determination of Piperine in Trikatu Churna: An Ayurvedic Formulation Asian J of Chem 2007;19(7):5331-5.

 

 

 

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Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

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This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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