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IJCRR - 3(5), May, 2011

Pages: 73-77

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CALIBRATION CURVE METHOD DEVELOPMENT FOR ANALYSIS OF TENATOPRAZOLE

Author: Sumit M. Deshmukh, Hemlata M. Nimje, Rajesh J. Oswal, Rishikesh V. Antre

Category: Healthcare

Abstract:A simple and sensitive UV spectrophotometric method has developed for quantitative estimation
of Tenatoprazole of pure drug. Tenatoprazole is antacid. Chemically it is (RS)-3-Methoxy-8-[(4-
methoxy-3,5-dimethyl-pyridin-2-yl)methylsulfinyl]-2,7,9 triazabicyclo[4.3.0]nona-2,4,8,10-
tetraene. Spectral absorbance measurements were made on Systronics double beam UV-VIS
Spectrophotometer with 1 cm matched quartz cells. Tenatoprazole was dissolved in distilled
water and absorbance was measured at 314.0 nm. Beer Lambert's law was obeyed in the
concentration range of 0-50\?g/ml. The method was statistically evaluated for accuracy and
precision.

Keywords: UV visible spectroscopy, Tenatoprazole, Calibration curve method

Full Text:

INTRODUCTION

UV light is defined as electromagnetic radiation having a wavelength less than that of visible light (400 nm) and greater than that of X-Rays (100 nm). The unit of wavelength used is a nanometer (nm) equal to 10-9 meters. Ultraviolet (UV) astronomy concerns celestial observations made in the region of the electromagnetic spectrum1,2 that extends from the near-visible to the Xray regime: the near UV, with wavelengths of 3500 to 2000 A0 the far UV (2000-912 A 0 ) and the extreme UV (912-100 A0 ). The main types of instruments in used for measuring the emission or absorptions of radiant energy from substance are called by various names such as Photometers, is the instrument which measures the ratio, or some function of the two, of radiant power of electromagnetic beam. Tenatoprazole is a prodrug of the proton pump inhibitor (PPI) class, which was converted to the active sulfonamide or sulfenic acid by acid in the secretory canaliculus of the stimulated parietal cell of the stomach. This active species binds to luminally accessible cysteines of the gastric H + , K+ -ATPase resulting in disulfide formation and acid secretion inhibition3,4,5.

Structure of Tenatoprazole Drugs which are in solid dosage forms are widely used for the treatment of various diseases & hence analyst needs to develop suitable identification method for analysis. A certain quantity dose of drug was just introduced in the market as tablet dosage for inhibit excess acid secretion. The efficacy of drug is more the same category so to inhibit the adulteration of drug or to increase the efficacy of drug the standard analysis of drug should be known by Pharmacist. Hence in the present research project attempt has been taken to develop simple, accurate, precise, sensitive as well as less time consuming identification method for simultaneous estimation of the drug Tenatoprazole in tablet dosage form in future6 .

Experimental Method7 Calibration curve can be done by Simultaneous equation methods or Vierodt‘s method, Multi-component method of analysis, Derivative Spectrophotometery, Geometric correction method, Absorption factor method, Difference Spectrophotometry, Orthogonal polynomial functional method, Absorbance ratio method & two wavelength/three wavelength method.

Calibration Curve Method

1) Selection of solvent: Distilled water is used as solvent.

2) Study of spectra, selection of maximum wavelength for Tenatoprazole:

I. Preparation of standard stock solution: An accurately weighed quantity, 100mg, of Tenatoprazole was placed in a 100 ml volumetric flask and dissolved in Distilled water. The volume was made up to mark with Distilled water to get concentration of 1000 µg/ml.

II. Study of spectra and selection of maximum wavelength (λ max) The aliquots of standard stock solution of Tenatoprazole was diluted with 100 ml Distilled water to obtain concentration 10µg/ml Solution was taken in 1cm cell and scanned in range 200nm to 400nm.

III. Study. of Beer-Lambert’s law Accurately measured aliquots of standard stock solution ranging from 0.1ml to 4ml were taken in series of 10ml volumetric flask and diluted up to Distilled water the mark with to get Concentration in range of 1-40 µg/ml. The absorbance of each solution was measured at 314.0nm.

RESULTS

DISCUSSION AND CONCLUSION

In distilled water, Tenatoprazole exhibits absorption at 314.0 nm. The linearity was observed in concentration range of 0-40 µg/ml. The amount of Tenatoprazole estimated by proposed method was in good agreement with the label claim. The low % RSD value indicates that method is accurate. The proposed method is simple, accurate, economical and be used in routine analysis of Tenatoprazole from tablet formulation.

ACKNOWLEDGMENT

The authors are thankful to Emcure Pharmaceutical Industry, Bhosari, Pune for providing the pure drug samples and Mr. Pravin Chavan for kind cooperation. Authors are also thankful to Prof. T. J. Sawant, founder secretary Jaywant Shikshan Prasarak Mandal`s for providing laboratory facilities.

References:

1. The United State‘s Pharmacopoeia 29/National formulary 24, The united state pharmacopoeial convention, Rockville, 2006. Page no. 3050-3052.

2. Friedel, Robert, and Paul Israel. 1987. Edison's electric light: biography of an invention. New Brunswick, New Jersey: 77 International Journal of Current Research and Review www.ijcrr.com Vol. 03 issue 05 May 2011 Rutgers University Press. Page no. 115- 117

3. Joshi M, Nikalje AP, Shahed M, Dehghan M, HPTLC method for the simultaneous estimation of Tenatoprazole, Indian Journal of Pharmaceutical Sciences, 71, 1, 2009, 95- 97.

4. Ashithosh Kar Pharmaceutical Drug Analysis New Age International Publishers,Delhi,2nd ed2005,Page no:303-306

5. Gurdeep R.Chatwal and Aham K.Anand Instrumental mehods of chemical analysis Himalaya publishing house, Mumbai,5thed. 2008,Page No-2.118-2.29

6. Conners K. A., Textbook of Pharmaceutical Analysis, 3 rd Edition, A Wiley-Intersciences Publication, 1999,Page no: 616-622.

7. Beckette A.H. Stenlake J.K. Practical Pharmaceutical Chemistry, Fourth edition, CBS publisher‘s and Distributor‘s, New Delhi, 1997, Page no.275-336.

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