International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - Vol 03 Issue 05, May, 2011

Pages: 64-72

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Author: Mary Josephine Rani A, John Milton MC, Uthiralingam M , Azhaguraj R

Category: General Sciences

Abstract:In the present study Clarias batrachus was exposed to sublethal concentrations (~10% of 96 h-
LC50) of mercury (0.080ppm) and chromium (10.2ppm) were investigated for 28 days under
laboratory conditions. The concentrations of these metals in the various organs were detected
through Inductively Coupled Plasma\?Atomic Emission Spectrometer (ICP-AES). The
concentration of mercury was high in brain (656.82ppb) followed by gills (287.63ppb), liver
(229.64ppb), kidney (176.44ppb) and muscles (49.21ppb) where as in the fishes exposed to
chromium, the concentration was high in gills (315.21ppb) followed by liver (241.4ppb), kidney
(187.96ppb), brain (128.29ppb) and muscles (51.3ppb).The ability of mercury and chromium as
toxicants was proved in the present study as the concentrations was found to be high in brain and
gills respectively.

Keywords: Clarias batrachus, heavy metals, ICP-AES

Full Text:


Over the last few decades, there has been growing interest in determining heavy metal levels in the aquatic environment and attention was drawn to the measurement of contamination levels in public food supplies, particularly fish. Metals are nonbiodegradable and are considered as major environmental pollutants causing cytotoxic, mutagenic and carcinogenic effects in animals (Kamaruzzaman, et al., 2010). Heavy metal contamination may have devastating effects on the ecological balance of the recipient environment and a diversity of aquatic organisms (Farombi, et al., 2007). Mercury, the black listed element by environmentalists, is released into the environment by several sources, such as mining and fossil fuel combustion, thermal power project, by the use of fungicides, bactericides and pharmaceuticals (Khangarot, 2003). A great deal of research concerning the effects of mercury on terrestrial and aquatic biota has demonstrated the potential risk that it represents because of its toxicity, accumulation and its tendency to biomagnify and also because of its properties like mobility and transformation in the environment, and its presence in humans (Francisco, 2004). Mercury in the organic form is the most toxic as it passes the blood brain barrier owing to its lipid solubility (Farhana et al., 2005). These heavy metal ions can exert their toxic effect through the gills, liver, kidney and brain (Velma et al., 2009). Chromium is widely used in industries, like leather tanning, electroplating, paint and pigment manufacturing, textile and fertilizers (Cervantes et al., 2001). Hexavalent chromium a heavy and poisonous ion is widely used, in manufacturing of steel as an electroplated coating for corrosion prevention; as a mordant in the textile industries; as an anticorrosive agent in the tanning industry, as catalyst for the manufacture of pigments and paints; in fungicides and wood preservatives and in anodization of aluminum in the aircraft industry ( Velma et al ., 2009).The hexavalent form is considered as the toxic form of chromium because it readily crosses cell membrane. Inside the cell, the hexavalent form is reduced to the trivalent form which complexes with intracellular macromolecules, including genetic material, and is ultimately responsible for the toxic and mutagenic capacities of chromium . The bioaccumulation of heavy metals and their toxic effects have been reviewed by Waqar 2006; Rasmussen and Anderson, 2000. Aquatic organisms have the ability to accumulate heavy metals from various sources including sediments, soil erosion and runoff, air depositions of dust and aerosol and discharges of waste water (Labonne et al., 2001). Therefore, accumulative capacity of aquatic organisms can pose a long lasting effect on biogeochemical cycling in the ecosphere. Heavy metals can also adversely affect the growth rate in different fishes (Hayat et al., 2007). The organisms developed a protective defense against the deleterious effects of essential and inessential heavy metals and other xenobiotics that produce degenerative changes like oxidative stress in the body (Abou EL-Naga et al., 2005; Filipovic and Raspor, 2003). Clarias batrachus (Linn.) is an air-breathing catfish, C. batrachus occurs in fresh and brackish waters throughout India. It is highly valued as a table fish throughout the Indian subcontinent and is preferred for culture even in muddy and shallow waters (Debnath and Surajit, 2009). The present study was carried out for a period of 28 days to determine the bioaccumulation of mercury and chromium in the various organs of Clarias batrachus.


Healthy living specimens of Clarias batrachus were procured, from Bharath Fish Seed India, Poondi, Thiruvallur district, Tamilnadu. C. batrachus measuring 9.5+ 0.5cm in length and weighing 5 + 0.5 gram were used in the experiment. The fishes were acclimatized to the laboratory conditions for 20 days prior to experiment in a glass aquarium (40 x 40 x 100 cm) filled with dechlorinated water. Water quality characteristics were determined. The mean values for the test water qualities were as follows, temperature 27.5±1.5C°, pH 7.5±0.03, dissolved oxygen 6.4±0.2mg/l, alkalinity 250±2.8mg/l as calcium carbonate(CaCo3), total hardness 456±3.5mg/l. Stock solutions of mercuric chloride and potassium dichromate were prepared by dissolving analytical grade mercuric chloride (HgCl2 from Merck) and potassiumdichromate (K2Cr2O7·7H2O from Merck) respectively in double distilled water. Acute toxicity test was conducted in accordance with standard methods (APHA, 1995). Sublethal or safe level concentrations were derived from 96 hours Lethal Concentration 50 (LC50) as per the procedure described by Finney (1971). The 96h LC50 were selected as sublethal concentrations for mercury (0.080ppm), chromium (10.2ppm) and ten fishes of same size were exposed to each concentration with three replicates for a period of 28 days. A control batch corresponding to each test group was simultaneously maintained. The experiments were repeated five times for each test concentrations. To maintain a constant toxic concentration in the media, the water medium with appropriate concentration of heavy metal was changed every day. Fish were fed with commercial pelleted diets ad libitum. After 28 days the gills, liver, kidney, brain and muscle tissues of control and treated C. batrachus were dissected and dried, the samples were processed for acid digestion by using perchloric acid and nitric acid in the ratio1:3. The digested tissue samples were made up to 25 ml and 1 ml of the sample was introduced into Inductively Coupled Plasma–Atomic Emission Spectrometer (ICP-AES) (ISA JOBIN YVON 24 MODEL). The obtained data were tabulated in order to get bioaccumulation in ppb levels (Topping, 1973).


The median lethal concentration (LC50) of mercury to Clarias batrachus for 24, 48, 72 and 96 hours of exposure are 1.4 ppm, 1.2 ppm, 1.0 ppm and 0.8 ppm respectively (Table 1). The LC50 value of chromium to C. batrachus for 24, 48, 72 and 96 hours of exposure are 120 ppm, 115 ppm, 110 ppm and 102 ppm respectively (Table 1). Increase in exposure period results in decreased LC50 values. The sublethal concentrations (~10% of 96 h-LC50) of mercury (0.080ppm), (~10% of 96 h-LC50) chromium (10.2ppm) were treated to C. batrachus for 28 days. Table 2 and figure 1 summarise the bioaccumulation of mercury and chromium in ppb levels in the various organs of control and treated Clarias batrachus. It is evident from the table that both mercury and chromium are consumed by the fish from the surrounding medium and accumulated in the various organs. In the mercury treated tissues of C. batrachus, the bioaccumulation was significantly higher in the brain (656.82 ppb) and lower in the muscles (49.21 ppb). The levels of mercury accumulation in the gills, liver and kidney were 287.63 ppb, 229.64 ppb and 176.44ppb respectively. The bioaccumulation of mercury in the tissues followed the order: Brain> Gills > Liver > Kidney > muscle. Bioaccumulation of chromium was significantly high in the gills (315.21.ppb) and low in the muscle (51.30 ppb). The levels of chromium accumulation in the liver, kidney and brain were 241.4 ppb, 187.96ppb and 128.29ppb respectively .The bioaccumulation of chromium in the tissues followed the order: Gills > Liver > Kidney > Brain > muscle.


Metals cannot be metabolized and therefore have the potency to accumulate in the body, leading to chronic effects. The only way in which the metals can be eliminated is by excretion. The rate and pathway of excretion vary greatly from one metal to another and between different tissues (Goyer, 1992).

According to Ferard et al. (1983) aquatic organisms take up heavy metals and concentrate in amounts considerably higher than found in the environment. Measurement of metals in aquatic environment is an important monitoring tool to assess the extent of pollution of the aquatic biotopes (Kumar and Mahadevan, 1995). The field of heavy metal detection by ICP-AES in the industrial waste and soilsediment is still very much in the stage of development (Shrivastava and Chaudhary, 2000). ICP-AES determination of metals is of high sensitivity and accuracy with low matrix effect and simple operation. Mercury in the aquatic ecosystem and its bioaccumulation as methylmercury at higher trophic levels are strongly influenced by the uptake of bioavailable forms of mercury by bacteria enhanced levels of mercury have since been found in fish (Nsikak et al., 2007). Mercury has the potential to bioaccumulate in food chains; the representative selection of an indicator species for a specified ecosystem appears to be a crucial step for reliable interpretation, particularly in long-term studies (Dusek, 2005). Increasing accumulation of mercury in tissues over the lifetime of examined fish can be regarded as an indication of contamination (Rincon et al., 1993; Niimi and Kissoon, 1994; Mueller and Serdar, 2002) and age-related changes then represent a crucial variability component in biomonitoring studies. The accumulation of mercury in the tissues of Clarias batrachus followed the order: Brain > Gill > Liver > Kidney > Muscle. As per the observations made, the accumulation of mercury occurs considerably on 28th day of exposure and significant accumulation takes place in the brain (Table 3 and Figure 1). A possible explanation could be that mercury has a high affinity for the sulfhydryl methyl group compound (Carty and Malone, 1979). Takeuchi (1982) suggested that methylmercury affects the nerve cells during the intermediate or late period of animal fetal life. The present study showed a pronounced change in the brain than in liver and kidney on the 28th day of exposure to mercury. Highest accumulation of mercury in the brain was observed when compared to the liver and kidney. This is because the high affinity of the neurosecretory cells of brain and its strong binding affinity towards mercury. Mercury can easily cross over the blood-brain-barrier system (Smith et al., 1987). Nsikak (2007) indicated in his finding that the accumulation of mercury was significantly higher in liver tissues of Pomadasys jubelini and Oreochromis nilotica. The present observation determined a low molecular bioaccumulation of mercury in the kidneys on the 28th day of exposure to the toxicant. This could be attributed to the low activity and functioning of the kidney in the C. batrachus. The slow blood flow into the renal tissue may lead to less accumulation of mercury in the kidney of the fish (Spitzer, 1985). At the lowest mercury concentration, the metal content was similar in gills, liver and kidneys and they were all statistically different from muscle (Antonia et al., 2003). Romeo et al. (1999) showed that mercury concentrations in edible muscles of pelagic fish species are lower than those of benthic fish species. It is generally accepted that muscle is not an organ in which metals accumulate. Similar results were reported from a number of fish species showing that muscle is not an active tissue in accumulating heavy metals (Karadede and unlu, 2000; Kargin and Erdem, 1991). Chromium is dangerous as it can accumulate in many organisms. The hexavalent chromium behaves toxicologically in a manner quite different from most heavy metal because hexavalent chromium can readily penetrate gill membranes passively and concentrate at high levels in various organs and tissues and can manifest its toxic action internally as well as on the gill surface (Buhler et al. 1977). In the present study the effects of sublethal concentration of chromium on the freshwater fish C. batrachus has been investigated to elucidate the persistence of chromium induced effects during chronic long term exposure. Maximum bioaccumulation was observed in the gill followed by liver, kidney, muscle and brain after 28 days of exposure to chromium (Table 1 and Figure 1). The gills generally showed highest metal concentrations, due to their intimate contact with the environment and their importance as effectors of ionic and osmotic regulation. The degree of chromium bioaccumulation in tissues suggests that the gills take up chromium more readily. Increased bioaccumulation in the gills may be due to the constant and increased ventilatory movements of the operculum under the influence of the xenobiotics. The protective mucous plug inside the opercular chamber is quite often discharged into the medium. Such discharges of mucous plug might make the gills a more vulnerable site for accumulation (Paul and Banerjee 1997). The accumulation of chromium in gill tissue is usually associated with structural damage to the gill epithelium as well as impaired respiratory and osmoregulatory function. These effects have often been cited as the acute mechanism of metal toxicity (Burton et al., 1972). Liver is the tissue with the second highest chromium accumulation. The liver, in its role as a storage and detoxification organ, can also accumulate high levels of metals (Nussey et al., 2000). It is suggested that in the liver chromium is stored and linked to proteins and smaller peptides such as glutathione (Gauglhofer and Bianchi, 1991). According to Heath, (1987) fish excrete chromium via their feces, as shown by high levels in bile during and after the ingestion of contaminated food or contaminated water. A higher concentration of chromium was mostly recorded in the gills followed by the liver. Muscles and skin accumulated much less metal concentrations (Nussey et al., 2000). The concentrations of chromium found in the gills, liver and muscle in this study also supported by various other study (Wepener, 1997). In the present study, the lowest chromium concentrations were mainly found in the muscles. This coincides favorably with the results from other study (; Wepener, 1997). The levels of chromium accumulations in the various organs is as follows Gills > Liver > Kidney > Brain > muscle. These observations agree with results obtained in similar study by (Avenant-Oldewag and Marx 2000) for Clarias gariepinus. The present findings are in perfect harmony with the above results. Thus the transformation, mobilization transport and bioaccumulation of chromium are of fundamental environmental significance.



In this study, it was clearly shown that the mercury and chromium exposure is having a significant impact on the brain and gills of the C. batrachus. Knowledge of these 69 International Journal of Current Research and Review Vol. 03 issue 05 May 2011 outputs made a possibility to give a better description of the variation and the pattern of metal accumulation in the various organs of C. batrachus. The scientific data discussed in this study provide a basis for understanding the potential impact, as well as for advancing our knowledge of the ecotoxicology and risk assessment of mercury and chromium.




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A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
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A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
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A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award

A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award

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Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.


International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal


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