International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, CiteFactor, Open J-Gate, ROAD, Indian Citation Index (ICI), Indian Journals Index (IJINDEX), Internet Archive, IP Indexing, Google Scholar, Scientific Indexing Services, Index Copernicus, ResearchBib, Science Central, Revistas Medicas Portuguesas, EBSCO, BOAI, SOROS, NEWJOUR, ResearchGATE, Ulrich's Periodicals Directory, DocStoc, PdfCast, getCITED, SkyDrive, Citebase, e-Print, WorldCat (World's largest network of library content and services), Electronic Journals Library by University Library of Regensburg, SciPeople.

Search Articles

Track manuscript

Advertisement

Full Html

IJCRR - Vol 04 Issue 24, December, 2012

Pages: 52-58

Date of Publication: 30-Nov--0001


Print Article   Download XML  Download PDF

HOMOCYSTEINE LEVELS AND ROLE OF OXIDATIVE STRESS IN PRIMARY OPEN ANGLE GLAUCOMA

Author: Sri Hari Atti, A. Saseekala, Varun Siri A.

Category: Healthcare

Abstract:Objective: Primary open angle glaucoma is the most commonest form of glaucoma which leads to vision loss if untreated. It has been hypothesized that oxidative damage may be involved in the pathogenesis of glaucoma and homocysteine may also have a role in development of glaucoma. The aim of our present study is to evaluate the role of oxidative stress and Homocysteine in Primary open angle glaucoma patients. Methods: We tried to assess Serum Homocysteine levels and the role of oxidative stress in patients by estimating the levels of lipid peroxidation assessing plasma Malondialdehyde (MDA) levels and antioxidant status by reduced glutathione (GSH), Vitamin-E ,Vitamin-C in blood. For this, we have taken 50 cases of primary open angle glaucoma patients compared with 75 age matched controls . Results: There were significant increase in the levels of MDA and significant decreases in the levels of antioxidants like GSH whereas there are significant increases in the levels of vitamin-E and Vitamin-C in breast cancer patients when compared with controls. No statistical significant difference was observed with Serum Homocysteine levels in cases when compared with controls. Conclusion: Our results indicate that oxidative stress is associated with the development of Primary open angle glaucoma which needs further studies.

Keywords: Oxidative stress, Malondialdehyde, Reduced glutathione (GSH) , Vitamin-C, Vitamin-E and Homocysteine

Full Text:

INTRODUCTION
Glaucoma is an eye disease in which the optic nerve is damaged in a characteristic pattern leading to permanently damaged vision in the affected eyes and blindness if left untreated. Glaucoma is the second leading cause of vision loss in the world affecting approximately 70 million people worldwide.(1) .Primary open angle glaucoma affects 1 to 2 percent of the population over the age of 40(2) which is the most common form of glaucoma. Elevated intraocular pressure due to reduction in aqueous outflow facility is the most significant risk factor. High intra ocular pressure usually occurs as a result of an increase in aqueous humor outflow resistance in trabecular meshwork. Recent evidences indicate that vascular risk factors may also play a role. Impaired microcirculation and abnormal perfusion may cause glaucomatous damage in the optic nerve head. Anatomical or functional abnormalities of the vessels of the optic nerve head such as arteriosclerosis or vascular dysregulation might be the causative factor(3,4). Homocysteine is an intermediary aminoacid formed during the conversion of methionine to cysteine. Elevated total plasma level of aminoacid Homocysteine has been identified as an independent risk factor of arteriosclerosis involving coronary, cerebral ,and peripheral arteries. High levels of Homocysteine causes lipid peroxidation, vascular endothelial injury, impaired vasomotor regulation, pro thrombotic surface, and therefore atherothrombogenesis.(5) Hyperhomocysteinemia is suggested to be an independent risk factor for premature vascular disease(6),myocardial infarction(7), and stroke(8).Furthermore, raised Homocysteine levels have recently been suggested as a risk factor for non-arteritic anterior ischemic optic neuropathy(9) and retinal vascular occlusive disease with thromboembolism.(10). Recently, higher levels of plasma Homocysteine in primary open angle glaucoma(11), pseudo exfoliation syndrome and glaucoma patients(12,13) were reported. The trabecular meshwork of patients with primary open angle glaucoma is characterized by specific morphologic and biochemical changes such as loss of trabecular meshwork cells, accumulation of extra cellular matrix ,and accelerated senescence.(14).It is assumed that these changes lead to an increased outflow resistance and thus to elevated intraocular pressure. The reasons for these changes are not clear. Various factors may play an elementary role in the pathologic course of the disease, such as genetic factors, increased levels of glutamate, changes in nitric oxide metabolism, and vascular changes. One factor which is increasingly important in the pathogenesis of primary open angle glaucoma is oxidative stress.(15). Oxidative stress represents a harmful state defined by the presence of pathologic levels of reactive oxygen species relative to antioxidant defense. Reactive oxygen species are molecules that oxidize more strongly than oxygen (o2) itself, or molecules containing oxygen that generate free radicals.ROS include superoxide, hydrogen peroxide and hydroxyl radical, which is the strongest oxidant produced in biological systems. Other reactive species, reactive nitrogen species include nitric oxide and peroxynitrite, a product resulting from the reaction between ROS and nitric oxide. Free radicals can be generated in an exaggerated manner and can injure tissues and organs by interacting with lipids, proteins or DNA(16).To survive, the human body has developed a complex, efficient and highly adaptive antioxidant defense system that includes two categories of antioxidants enzymatic such as glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase and non enzymatic such as reduced glutathione, antioxidant vitamins and low molecular weight compounds such as urate. The eye is also protected against oxidative stress by several mechanisms involving antioxidant enzymes such as GSH and ascorbate.(17). Altered GSH and GSH activity has been reported in the trabecular meshwork and aqueous humor of patients with glaucoma.(18).

MATERIALS AND METHODS
The present study was conducted in department of ophthalmology and the department of Biochemistry , S.V.Medical college ,Tirupati. A total of 50 cases of primary open angle glaucoma patients belonging to the age group of 30-50 years were selected for this study. Age and sex matched 75 normal patients without glaucoma were selected as controls. Informed consent from all cases and controls were obtained. Ethical clearance was obtained for this study A detailed medical history was obtained to identify those with known or suspected diabetes mellitus, systemic hypertension, peripheral or coronary artery disease, venous thrombotic events, or cerebrovascular disease, and current drug therapy. Exclusion criteria included diabetes mellitus, systemic hypertension, peripheral or coronary artery disease, cerebrovascular disease ,major systemic illness, evidence of vasculitis, renal or hepatic disease, gastrointestinal malabsorption, cardiomyopathy, psychiatric illness, chronic alcohol abuse, smoking, anticonvulsant and immunosuppressive therapy, postmenopausal hormone replacement, current use of cholesterol lowering drugs, antidepressants, antimicrobial therapy and vitamin supplements. All subjects underwent a complete ophthalmic examination including visual acuity, slit lamp examination, intraocular pressure measurement using Goldmannn applanation tonometry, gonioscopy, and dilated fundoscopic examination and systemic examination. Inclusion criteria were as follows: Primary open angle glaucoma was defined by the presence of an open angle on gonioscopy, IOP≥22mm of Hg measured with a Goldman Applanation Tonometer, typical glaucomatous cupping and visual field defect in at least one eye on standard automated perimetry. Control subjects had no history of elevated IOP higher than 22 mm of Hg, no exfoliative material on the anterior lens capsule, normal visual fields and optic discs. 10ml of fasting blood samples were collected by venipuncture and for the separation of sera, 5ml of blood was centrifuged at 3000rpm for 5min and the remaining 5ml of blood was taken into a plain vial containing EDTA and was centrifuged at 3000rpm for 10min for the separation of plasma. The plasma MDA levels were estimated by using thiobarbituric acid reacting substances(TBARS) by the method of Yagi(19) and Sinnhuber et al(20).Reduced glutathione was determined by the method of Beutler et al(21). Serum Vit-E was measured by the method of Baker on the basis of reduction of ferric ions to ferrous ions by Vit-E and the formation of red colored complex with 2-2` dipyridyl at 520nm(22,23).The activity of Ascorbic acid was determined by the method of Tietz(24). Serum total homocysteine levels were determined by enzyme linked immunosorbent assay(ELISA) kit .All the results were expressed as mean ± SD and statistical comparisons were done using student t-test using the SPSS package. 

Evaluation of oxidative stress is done based on the levels of MDA and statistically significant increase in the level of MDA was observed in open angle glaucoma patients when compared to controls. Statistically significant decreases were observed in the levels of enzymatic antioxidants like GSH and statistically significant increases in the levels of nonenzymatic antioxidants vitamin-E,C in cases when compared to controls. There was no statistical significant difference in the levels of serum homocysteine in cases when compared with controls. DISCUSSION Glaucoma, being a non systemic disease, is the most frequent etiology of irreversible blindness worldwide is an ocular pathology. Studies related to its effects on serum oxidative stress markers are quite limited in the existing literature. In this study, the role of homocysteine and the effects of oxidative stress and antioxidant status in glaucoma were studied. Glaucoma is an optic neuropathy, and oxidative stress plays an important role in its etiopathogenesis. In our study, a statistically significant relationship was found between the presence of primary open angle glaucoma and plasma MDA levels. The plasma MDA level, a byproduct of lipid peroxidation, is a reliable and commonly used biomarker of overall lipid peroxidation. As there are high aqueous concentrations of hydrogen peroxide and photochemical reactions in the anterior segment arising from aerobic metabolisms, the trabecular meshwork is exposed to high levels of oxidative stress.(25) Aqueous humor is known to contain several active oxidative agents such as hydrogen peroxide and superoxide anion(26).It has been suggested that chronic oxidative stress induced by such agents can compromise trabecular meshwork functions (27) and subsequently play a role in the pathogenesis of primary open angle glaucoma. Our finding of increased plasma MDA levels in primary open angle glaucoma patients is not only consistent with the role of oxidative stress in glaucoma but also supports the idea that plasma MDA levels may be used as a marker of oxidative stress on a group basis. We also observed significant decrease in the levels of reduced glutathione in cases when compared to controls. Circulating GSH can be depleted either by subjecting cells to oxidative stress, or by inhibition of synthesis. In patients with glaucoma , due to a high level of oxidative stress,(28) GSH could be overused implying a reduction of protection against ROS, possibly due to a defective redox cycle. Another possible explanation for reduced GSH levels may be due to defective intracellular synthesis. The liver is the major site for GSH synthesis. The precursors necessary for this synthesis are Lglutamate, L-cysteine and L-glycine. Although both glutamate and glycine are important, it seems that the major determinant of the rate of GSH synthesis is the availability of the amino acid cysteine.(29).Cysteine results from the metabolism of homocysteine and any interruption in the homocysteine-cysteine pathway would result not only in the accumulation of homocysteine but also in less available quantities from the second amino acid which may affect GSH synthesis. There is a significant increase in the levels of vitamin-E in cases when compared to controls. Vitamin-E acts as antioxidant and many in vivo and in vitro studies performed in normal and neoplastic cells have demonstrated that α –tocopherol had special effects, including gene regulation(30).This phenomenon also applies to ocular tissues.(31). It also has been established that vitamin-E derivatives act as neurohormones, and initiate various intracellular conduction pathways with a lock and key model. Retinal blood flow regulating and neuroprotective effects of vitamin-E in glaucoma patients have been clinically demonstrated.(32). Moreover, vitamin-E is recognized for prolonging life span in retinal cell cultures(33).Taking all the above factors into consideration, the elevation of vitamin-E in glaucoma indicates the exaggerated response of antioxidants to oxidative stress in glaucoma. There is also a increase in the levels of non-enzymatic anti-oxidants such as Vit-C, which states that there is an increased defense mechanism against oxidative damage in breast cancer. The increase in the levels of these non-enzymatic antioxidant parameters may be due to an increased turnover for preventing oxidative damage in these patients, thus suggesting an increased defense against oxidative damage. Our results support the researchers who showed over expression of antioxidants(34).Several other researchers reported decreases in the antioxidant level and increases in lipid peroxidation level(35). No statistical difference was observed in the levels of homocysteine levels in cases when compared with controls. Some studies have reported elevated serum homocysteine level in primary open angle glaucoma patients and suggested that thermo labile methylene tetrahydrofolate reductase deficiency may be in part the cause of the increased serum homocysteine level in patients with primary open angle glaucoma.(36).Our findings supports the study of researchers who observed no difference in homocysteine levels.(37). In conclusion, the present study revealed an increase in the levels of MDA, vitamin-E,C and decrease in the levels of reduced glutathione with no alterations in the levels of serum homocysteine in cases compared to controls suggesting the role of oxidative stress as a pathogenic mechanism in the development of glaucoma and further extensive studies are required in future to establish oxidative stress as a biomarker in the development of primary open angle glaucoma.

ACKNOWLEDGEMENTS
Authors acknowledge the immense help received from the scholars whose articles are cited and included in references of this manuscript. The authors are also grateful to authors/editors/publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed.

 

References:

1. Quigley HA.Number of people with glaucoma world-wide.Brit J Ophthalmol 1996;80:389-393.

2. Leske MC. The epidemiology of open angle glaucoma: a review. Am J Epidemiol 1983; 118:166-91.

3. Flammer J,Orgul S, Costa VP, Orzalesi N, Krieglstein GK, Serra LM et al. the impact of ocular blood flow in glaucoma. Prog Retin Eye Res 2002,21:359-393.

4. Hayreh SS: Blood flow in the optic nerve head and factors that may influence it.Prog Retin Eye Res 2001,20:595-624.

5. Hankey GJ, Eikelboom JW. Homocysteine and vascular disease. Lancet 1999; 354:407- 13.

6. Clarke R,Daly L, Robinson K, Naughten E et al. Hyperhomocysteinemia:an independent risk factor for vascular disease.N Engl J Med 1991;324:1149-1155.

7. Stampfer MJ, Malinow MR, Willett WC,Newcomer LM et al.A prospective study of plasma homocysteine and risk of myocardial infarction in US physicians.JAMA 1992;268:877-881.

8. Perry IJ, Refsum H, Morris RW et al.Prospective study of serum total homocysteine concentration and risk of stroke in middle -aged British men.Lancet 1995;346:1395-1398.

9. Kawasaki A,Purvin VA,Burgett RA.Hyperhomocysteinemia in young patients with non-arteritic anterior ischemic optic neuropathy. Br J Ophthalmol 1999; 83:1287-90.

10. Cahill M,Karabatzaki M, Meleady R,et al.Raised plasma homocysteine as a risk factor for retinal vascular occlusive disease.Br J Ophthalmol 2000;84:154-7.

11. Bleich S,Junemann A,Von Ahsen N,Lausen B,Ritter k et al.Homocysteine and risk of open angle. J Neural Transm 2002; 109:1499-1504.

12. Leibovitch I, Kurtz S,Shemesh G et al. Hyperhomocysteinemia in pseudo exfoliation glaucoma.J Glaucoma 2003;12:36-39.

13. Vessani RM,Ritch R,Liebmann JM, Jofe M.Plasma homocysteine is elevated in patients with exfoliation syndrome. Am J Ophthalmol 2003;136:41-46.

14. Liton PB, Challa P,Stinnett S, Luna C et al.Cellular senescence in the glaucomatous outflow pathway.Exp Gerontol. 2005; 40:745-748.

15. Izzotti A, Bagnis A, Sacca SC. The role of oxidative stress in glaucoma.Mutat Res.2006;612:105-114.

16. Valencia E, Hardy G, Marin A. Glutathione: nutritional and pharmacologic viewports:Part VI.Nutrition.2002;18:291- 292.

17. Richer SP,Rose RC.Water soluble antioxidants in mammalian aqueous humor:interaction with UV and hydrogen peroxide.Vision Res.1998;38:2881-2888.

18. Izzotti A,Sacca SC,Cartiglia C,De Flora S. Oxidative deoxyribonucleic acid damage in the eyes of glaucoma patients.Am J Med.2003;114:638-646.

19. Yagi K. Lipid peroxides and human diseases. Chem Phys Lipids, 1978; 45: 337- 351.

20. Sinnhuber RO, Yu TC. Characterization of red pigment formed in thiobarbituric acid determination of oxidative rancidity. Food Res, 1958; 23: 626-630.

21. Beutler E, Duron O, Kelly BM. Improved method for determination of blood glutathione. J Lab Clin Med, 1963; 61:882- 888.

22. Baker H, Frank O, Angelis B ,Feinfold S. Plasma tocopherol in man at various times after ingesting free or acylated tocopherol. Nutrition report international 1980; 21:531-536.

23. BakerandFrank. Determination of serum tocopherol by colorimetric method. In Varley’s practical clinical biochemistry, Heinmann professional publishing 1998;6th edition.902.

24. Tietz NW(Ed). Textbook of Clinical Chemistry. W.B.Saunders Company, Philadelphia, London, Toronto.2004; pp.960-962.

25. Stephen B, Koh H, Phil M, Henson D.Boulton M. The role of oxidative stress in the pathogenesis of age related macular degeneration.Surv Ophthalmol 2000;45:115-134.

26. Spector A, Gamer WH.Hydrogen peroxide and human cataract.Exp Eye Res 1981; 33:673-681.

27. Nguyen KPV, Chung ML, Anderson PJ, Johnson M, Epstein DL. Hydrogen peroxide removal by the calf aqueous outflow pathway.Invest Ophthalmol Vis Sci 1988;29:976-981.

28. Ferreira SM,Lerner SF,Brunzini R,Evelson PA,Llesuy SF. Oxidative stress markers in aqueous humor of glaucoma patients. Am J Ophthalmol.2004;137:62-69.

29. Lu SC.Regulation of hepatic glutathione synthesis:current concepts and controversies.Faseb J.1999;13:1169-1183.

30. Traber MG,Packer L.Vitamin E beyond antioxidant function.Am J Clin Nutr 1995;62:1501s-9s 

31. Alvarez RA, Liou GI, Fong SL, Bridges CD. Levels of α- and γ-tocopherol in human eyes:evaluation of the possible role of IRBP in intraocular transport.Am J Clin Nutr 1987;46:481-7.

32. Engin KN,Engin G et al .Clinical evaluation of the neuroprotective effect of α-tocopherol on retina against glaucomatous damage.Eur J Ophthalmol 2007;17:528-33.

33. Rego AC,Santos MS,Proenca MT, Oliveira CR.Influence of Vitamin E succinate on retinal cell survival.Toxicology 1998;128:113-24.

34. Iscan M, Coban T, Cok I, et al. The organochlorine pesticide residues and antioxidant enzyme activities in human breast tumors: is there any association? Breast Cancer Res Treat, 2002;72:173.

35. Yeh CC, Hou MF, Tsai SM, et al. Superoxide anion radical, lipid peroxides and antioxidant status in the blood of patients with breast cancer. Clin Chim Acta 2005; 361:104-11.

36. Bleich S, Junemann A et al. Homocysteine and risk of open angle glaucoma. J Neural Transm 2002, 109:1499-1504.

37. Tongabay Cumurcu, Semsettin Sahin and Erdinc Aydin.Serum homocysteine,vitamin B12 and folic acid levels in different types of glaucoma.BMC Ophthalmology 2006;6:6:1471-2415.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by situs slot and Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh et al. situs slot "Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma situs slot entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by situs slot et al entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09
Late to bed everyday? You may die early, get depression situs slot
Egg a day tied to lower risk of heart disease situs slot
88 Percent Of Delhi Population Has Vitamin D situs slot  Deficiency: ASSOCHAM Report

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: We at IJCRR voluntarily adopt policies on Code of Conduct, and Code of Ethics given by OASPA and COPE. To know about IJCRRs Code of Conduct, Code of Ethics, Artical Retraction policy, Digital Preservation Policy, and Journals Licence policy click here

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



Company name

International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2022 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions

  1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Pragmatic Play
  4. Situs Slot Online Terpercaya
  5. Kumpulan Situs Judi Slot Terpercaya
  6. Game Slot Online
  7. Judi Slot Online Jackpot Terbesar
  8. Situs Judi Slot Online Terpercaya 2021
  9. Slot Joker123
  10. Agen Slot Online Resmi
  11. Slot Deposit Pulsa
  12. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  13. AloJudi Slot
  14. KayaMendadak88 Slot
  15. Situs Slot Gacor 2022
  16. Judi Slot Online Jackpot Terbesar Gampang Menang
  17. Joker123 Slot
  18. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  19. Mesin slot gacor 88
  20. Mesin slot
  21. Link Slot Online
  22. Slot Via Dana
  23. situs judi slot terbaik dan terpercaya no 1
  24. Daftar Situs Bo Slot Online Gacor Terbaik 2022
  1. Slot Online
  2. Judi Slot Online Jackpot Terbesar
  3. Daftar Situs Judi Slot Online Terpercaya
  4. Judi Slot Terpercaya
  5. Kumpulan Situs Judi QQ Online Terpercaya
  6. Slot Online Terbaru
  7. Situs Game Slot Online Terbaik
  8. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  9. Situs Slot Deposit Pulsa dan Slot Online Terbaik
  10. Slot Hacker
  11. Situs Slot Online Terbaik
  12. Slot Gacor Gampang Menang
  13. Situs Judi Slot Online Gacor Terbaik 2022
  14. Situs Judi Slot Online Jackpot Terbesar
  15. Slot Online Terpercaya
  16. Game Slot Penghasil Uang
  17. Situs Slot Online Terbaik 2021
  18. Situs Slot Online Terbaik dan Terpercaya
  19. Situs Slot Terbaik
  20. Situs Judi Slot Terbaik Dan Terpercaya No 1