IJCRR - 9(3), February, 2017
Pages: 53-57
Microbial Fingerprinting - A current vogue in Microbial Forensics
Author: Moumita Sinha1, I. Arjun Rao2
Category: General Sciences
[Download PDF]
Abstract:
Classification and Identification of the microorganisms are of utmost importance in the field of environmental, industrial, medical and agricultural microbiology, microbial ecology and microbial forensic studies. Conventional phenotype-based methods come across many challenges and shortcomings which limit their usability. Molecular techniques offer better arrangements in recognizing and portraying microorganisms. A few DNA fingerprinting strategies have been produced and are being used as of now. In principle, most of these methods are based on PCR and restriction site analysis. Some of these methods are still not cost-effective in use and require huge set-up cost. Continuous research is going on around the world to improve the methodology and applicability of these methods as well as to make them economic and in routine use in forensic investigations..
Keywords: Microbes, Fingerprinting, DNA, RFLP, Multilocus
Citation:
Moumita Sinha1, I. Arjun Rao2. Microbial Fingerprinting - A current vogue in Microbial Forensics International Journal of Current Research and Review. 9(3), February, 53-57
References:
1. Bhatia Mohit, Mishra Bibhabati, Thakur Archana, Dogra Vinita, Loomba Poonam Sood. 2016. Concept of Forensic Microbiology and its Applications. Sikkim Manipal University Medical Journal. Vol. 3(1).
2. Budowle. B, Schutzer SE, Einseln A, Kelley LC, Walsh AC, Smith JA, et al. 2003. Building microbial forensics as a response to bioterrorism. Science. Public health. 301 (5641), 1852-3.
3. Hedrick David B, Peacock Aaron, Stephen John R, Macnaughton Sarah J, Bruggemann Julia, White David C. Measuring soil microbial community diversity using polar lipid q fatty acid and denaturing gradient gel electrophoresis data. Journal of Microbiological Methods 41 (2000) 235–248.
4. Muyzer G, de Waal EC, Uitterlinden AG. 1993. Profiling of microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695–700.
5. Osborn AM, Moore ERB, Timmis K. 2000. An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol. 2:39-50.
6. Interstate Toxicology, Regulatory Council, 2011. http:// www.itrcweb.org. Retrived on 17 December, 2016.
7. Daly M, Power E, Bjorkroth J, et al. 1999. Molecular analysis of Pseudomonas aeruginosa epidemiological investigation of mastitis outbreaks in Irish dairy herds. Applied and Environmental Microbiology. 65, 2723 -2729.
8. Tenover FC. 1985. Plasmid fingerprinting a tool for bacterial strain identification and surveillance of nosocomial and community acquired infections. Clinics in Laboratory Medicine. 5, 413-436.
9. Schaberg DR, Tompkins LS, Falkow S. 1981. Use of agarose gel electrophoresis of plasmid deoxyribonucleic acid to fingerprint gram-negative bacilli. Journal of Clinical Microbiology. 13, 1105-1110.
10. Fornasini M, Reeves RR, Murray BE, et al. 1992. Trimethoprim resistant Escherichia coli in households of children attending day care centers. Journal of Infectious Disease. 166, 326-330.
11. Pfaller M A, Wakefield DS, Hollis R, et al. 1991. The clinical microbiology laboratory as an aid in infection control. The application of molecular techniques in epidemiologic studies of methicillin resistant Staphylococcus aureus. Diagnostic Microbiology & Infectious Disease. 14, 209-214.
12. Maidenn MCJ, Bygrives JA, Feil E, et al. 1998. Muitilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences. USA. 95, 3140-3145.
13. Czajka J, Bsat N, Piana M, et al. 1993. Differentiation of Listeria monocytogenes and Listeria innocua by 16S genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms. Applied and Environmental Microbiology. 59, 304-308.
14. Young KA, Power EG, Dryden MS, et al. 1994. RAPD typing of clinical isolates of Staphylococcus haemolyticus. Letters in Applied Microbiology. 18, 86-89.
15. Warner JM, Oliver JD. 1999. Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other Vibrio species. Applied and Environmental Microbiology. 65, 1141-1144.
16. Welsh J, McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Research. 18, 7213-7218.
17. Welsh J, McClelland M. 1993. The characterization of pathogenic microorganisms by genomic fingerprinting using arbitrarily primed polymerase chain reaction (AP-PCR). In Diagnostic Molecular Microbiology (eds. Persing D. H. et al.) washington: ASM press. pp. 595- 602.
18. Williams JGK, Kubelik AR, Lival KJ, et al. 1990. DNApolymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research. 18, 6531-6535.
19. Tyler KD, Wang G, Tyler SD, Johnson WM. Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens. J. Clin. Microbiol., 35 (1997), pp. 339–346.
20. Madden RH, Moran L, Scates P. 1996. Sub-typing of animal and human Campylobacter spp. using RAPD. Letters in Applied Microbiology. 23, 167-170.
21. Enright MC, Spratt BG. 1998. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology. 144, 3049-3060.
22. Enright MC, , Griffiths D, Spratt BG. 1999. The three major Spanish clones penicillin-resistant Streptococcus pneumoniae are the most common clones recovered recent cases of meningitis in Spain. Journal of Clinical Microbiology. 37, 3210-3216.
23. Cocolin L, Manzano M, Cantoni C, Comi G. 2000. Development of a rapid method for the identification of Lactobacillus spp. isolated from naturally fermented Italian sausages using a polymerase chain reactiontemperature gradient gel electrophoresis. Letters in Applied Microbiology. 30, 126-129.
24. Tannock GW, Timisjarvi AT, Rodtong S. 1999. Identification of Lactobacillus isolates from the gastrointestinal tract, silage and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Applied and Environmental Microbiology. 65(9), 4264-4267.
25. Gurtler V, Stanisich, VA. 1996. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology, 142, 3-16.
26. Höfle M. 1988. Identification of bacteria by low molecular weight RNA profiles: a new chemotaxonomic approach. J Microbiol Methods 8: 235-248.
27. Höfle MG. 1998. Genotyping of bacterial isolates from the environment using low molecular weight RNA fingerprints. Mol Microb Ecol Manual 3.3.7: 1-23.
28. Höfle M. 1992. Bacterioplankton community structure and dynamics after large-scale release of non-indigenous bacteria as revealed by low molecular weight RNA analysis. Appl Environ Microbiol 58: 3387-3394.
29. Bidle KD, Fletcher M .1995. Comparison of free-living and particle-associated bacterial communities in the Chesapeake Bay by stable low-molecular-weight RNA analysis. Appl Environ. Microbiol 62: 944-952.
30. Wintzingerode F, Göbel UB, Stackebrandt E .1997. Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiol Rev 21: 213-229.
31. Höfle MG, Brettar I . 1996. Genotyping of heterotrophic bacteria from the central Baltic Sea by use of low-molecular-weight RNA profiles. Appl Environ Microbiol 62: 1383- 1390.
|